VALIDATION OF THE QUANTITATION METHODS OF 1-(β-PHENYLETHYL)-4-AMINO-1,2,4- TRIAZOLE BROMIDE SUBSTANCE BY SPECTROPHOTOMETRIC METHOD


Kucherenko Lyudmila Ivanovna, Parniuk Natalia Viktorovna, Khromylova Olga Vladimirovna

Abstract


 Objective: The aim of the work was to determine the validation characteristics for the developed method of quantitation of 1-(β-phenylethyl)-4- amino-1,2,4-triazole bromide substance by absorption spectrophotometry in the ultraviolet region (UV).

Methods: For study purposes, the 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide substance and the standard sample, obtained by the state enterprise “plant of chemical reagents” of scientific and technological complex “institute of single crystals” of the National Academy of Sciences of Ukraine, was used. In the course of our work, we used chemical, physical, physicochemical (absorption spectrophotometry in the UV region), and statistical methods of analysis. The study was carried out at the Laboratory for Standardization and Technology of Medicines at the Department of Pharmaceutical Chemistry at Zaporizhzhia State Medical University (Ukraine).

Results: In the previous studies, we have developed a technique for spectrophotometric determination of the quantitative content of 1-(β-phenylethyl)- 4-amino-1,2,4-triazole bromide substance. The results of the studies showed that the absorption curve in the UV region of the substance solution has three absorption maxima, namely: λ1=252 nm, λ2=258 nm, and λ3=263 nm. To quantify the substance, the wavelength λ2=258 nm was used. The results of the quantitation of the 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide substance by spectrophotometric method prove that the technique developed by us is accurate and reproducible and meets the current requirements. To the developed methodology to ensure accurate and accurate analysis results, its validation was carried out. According to the requirements of the State Pharmacopoeia of Ukraine (2 editions), the developed method for quantitation of the substance was checked for such validation characteristics as specificity, linearity, range of application, accuracy, correctness, and robustness.

Conclusions: As a result of the work, it was proved that the method of quantitation of 1-(β-phenylethyl)-4-amino-1,2,4-triazolе bromide substance by spectrophotometric method is valid.


Keywords


Arterial hypertension, Spectrophotometry, Quantitation, Validation, 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide.

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About this article

Title

VALIDATION OF THE QUANTITATION METHODS OF 1-(β-PHENYLETHYL)-4-AMINO-1,2,4- TRIAZOLE BROMIDE SUBSTANCE BY SPECTROPHOTOMETRIC METHOD

Keywords

Arterial hypertension, Spectrophotometry, Quantitation, Validation, 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide.

DOI

10.22159/ajpcr.2018.v11i2.22740

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 231-234

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Kucherenko Lyudmila Ivanovna
Department of Pharmaceutical Chemistry, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Ukraine

Parniuk Natalia Viktorovna
Department of Pharmaceutical Chemistry, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Ukraine

Khromylova Olga Vladimirovna
Department of Pharmaceutical Chemistry, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Ukraine


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