SCREENING FOR THE HOMOZYGOUS C.144DELC MUTATION IN AURKC GENE IN ALGERIAN INFERTILE MEN


Rezgoune Mohamed Larbi, Chellat Djalila, Abadi Noureddine, Satta Dalila

Abstract


 Objective: Male infertility is a major health problem worldwide. Despite recent advances, the etiopathogenesis of spermatogenic failure remains largely uncertain. Aurora kinases, a family of serine/threonine kinases, consisting of Aurora A (AURKA), Aurora B (AURKB), and Aurora C (AURKC), are essential kinases for cell division through regulating mitosis and meiosis. The aim of this study was to investigate the frequency of c.144delC mutation in AURKC gene in infertile Algerian patients with abnormal sperm parameters.

Methods: In this study, 40 infertile men with impaired spermatogenesis (Azoospermia “AZOs,”” oligoasthenoteratospermia “OATs,”” Asthenospermia “ASTs”) were recruited from Ibn Rochd clinic and Ibn Sina Laboratory, between 2008 and 2014. All men were of Algerian origin. DNA was extracted from peripheral blood. The third exon of the AURKC gene was amplified using polymerase chain reaction (PCR). Then, PCR products were sequenced using the big dye V1.1 terminator cycle sequencing in forward and reverse directions, and the results of sequenced segments were analyzed.

Results: Sequencing of the third exon of AURKC gene revealed the absence of c144delC mutation in all of the 40 patients screened.

Conclusion: Our data indicate that the AURKC c.144delC mutation must be investigated in infertile men with Macrozoospermia.


Keywords


AURKC, c144delC mutation, Male infertility, Sequencing, Algerian patients.

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About this article

Title

SCREENING FOR THE HOMOZYGOUS C.144DELC MUTATION IN AURKC GENE IN ALGERIAN INFERTILE MEN

Topics

biomedical, genetics, human infertility

Keywords

AURKC, c144delC mutation, Male infertility, Sequencing, Algerian patients.

DOI

10.22159/ajpcr.2018.v11i2.22783

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 161-163

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Rezgoune Mohamed Larbi
Laboratory of Molecular and Cellular Biology, University Constantine I, Constantine, Algeria.
Algeria

Chellat Djalila
Laboratory of Molecular and Cellular Biology, University Constantine I, Constantine, Algeria.
Algeria

Abadi Noureddine
Laboratory of Biology and Molecular Genetic, University Hospital Center IBN-Badis, University Constantine III, Constantine, Algeria.
Algeria

Satta Dalila
Laboratory of Biology and Molecular Genetic, University Hospital Center IBN-Badis, University Constantine III, Constantine, Algeria.
Algeria


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