ANALYSIS OF THE INHIBITION POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM THE SALIVA AND DORSUM OF THE TONGUE OF ADULTS ON THE GROWTH OF STREPTOCOCCUS MUTANS


Deborah Chintyanita, Hedijanti Joenoes, Boy M Bachtiar

Abstract


Objective: The aim of this study is to analyze the inhibitory potential of Streptococcus salivarius, after it has been isolated from saliva and the tongue’sdorsum, on the growth of Streptococcus mutans.

Method: Polymerase chain reaction, SDS PAGE, Bradford test, deferred antagonism test, and well-diffusion test were used.

Result: S. salivarius isolated from saliva and the tongue’s dorsum inhibited the growth of S. mutans, but it was not the case with S. salivarius ATCC13419 (p<0.05). However, there was no significant difference between the inhibition of S. mutans from S. salivarius isolated from saliva and thetongue’s dorsum (p>0.05).

Conclusion: S. salivarius isolated from saliva and the tongue’s dorsum can inhibit the growth of S. mutans, but there was no significant difference inthe inhibitory potential of S. salivarius isolated from saliva on the tongue’s dorsum.


Keywords


Probiotic, Streptococcus salivarius, Streptococcus mutans.

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References


Levy SB. The 2000 Garrod lecture. Factors impacting on the problem of antibiotic resistance. J Antimicrob Chemother 2002;49(1):25-30.

Anurogo D. Probiotik: Problematika dan progresivitasnya. Medicinus 2014;27(3):46-57.

Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005;43(11):5721-32.

Haukioja A. Probiotics and oral health. Eur J Dent 2010;4(3):348-55.

Goldin BR, Gorbach SL. Clinical indications for probiotics: An overview. Clin Infect Dis 2008;46 Suppl 2:S96-100.

Fauzi AA, Shafiei Z, Baharin B, Mohd N. Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens. Sains Malays 2013;42(1):19-24.

Burton JP, Wescombe PA, Moore CJ, Chilcott CN, Tagg JR. Safety assessment of the oral cavity probiotic Streptococcus salivarius K12.

Appl Environ Microbiol 2006;72(4):3050-3.

Horz HP, Meinelt A, Houben B, Conrads G. Distribution and persistence of probiotic Streptococcus salivarius K12 in the human oral cavity as determined by real-time quantitative polymerase chain reaction. Oral Microbiol Immunol 2007;22(2):126-30.

Kaci G, Goudercourt D, Dennin V, Pot B, Doré J, Ehrlich SD, et al. Anti-inflammatory properties of Streptococcus salivarius, a commensal

bacterium of the oral cavity and digestive tract. Appl Environ Microbiol 2014;80(3):928-34.

Di Pierro F, Zanvit A, Nobili P, Risso P, Fornaini C. Cariogram outcome after 90 days of oral treatment with Streptococcus salivarius

M18 in children at high risk for dental caries: results of a randomized, controlled study. Clin Cosmet Investig Dent 2015;7:107-13.

Indonesian Ministry of Health. InfoDATIN Data and Information Center of Indonesian Ministry of Health: Situasi Kesehatan Gigi dan

Mulut; 2014. Available from: http://www.depkes.go.id/download. php?file=download/pusdatin/infodatin/infodatin-gilut.pdf.

Bowe WP, Filip JC, DiRienzo JM, Volgina A, Margolis DJ. Inhibition of propionibacterium acnes by bacteriocin-like inhibitory substances

(BLIS) produced by Streptococcus salivarius. J Drugs Dermatol 2006;5(9):868-70.

Wescombe PA, Upton M, Dierksen KP, Ragland NL, Sivabalan S, Wirawan RE, et al. Production of the lantibiotic salivaricin A and its

variants by oral streptococci and use of a specific induction assay to detect their presence in human saliva. Appl Environ Microbiol

;72(2):1459-66.

Prasad S, Morris PC, Hansen R, Meaden PG, Austin B. A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio

harveyi. Microbiology 2005;151:3051-8.

Samaranayake L. Essential Microbiology for Dentistry. 3 ed. Edinburgh: Churchill Livingstone Elsevier; 2006.

Wescombe PA, Hale JD, Heng NC, Tagg JR. Developing oral probiotics from Streptococcus salivarius. Future Microbiol

;7(12):1355-71.

Roger P, Delettre J, Bouix M, Béal C. Characterization of Streptococcus salivarius growth and maintenance in artificial saliva. J Appl Microbiol 2011;111(3):631-41.

Wescombe PA, Heng NC, Burton JP, Chilcott CN, Tagg JR. Streptococcal bacteriocins and the case for Streptococcus salivarius as

model oral probiotics. Future Microbiol 2009;4(7):819-35.

Pag U, Sahl HG. Multiple activities in lantibiotics-models for the design of novel antibiotics? Curr Pharm Des 2002;8:815-33.

Chatterjee C, Paul M, Xie L, van der Donk WA. Biosynthesis and mode of action of lantibiotics. Chem Rev 2005;105(2):633-83.

Handley PS, Harty DW, Wyatt JE, Brown CR, Doran JP, Gibbs AC. A comparison of the adhesion, coaggregation and cellsurface

hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius. J Gen Microbiol 1987;133(11):3207-17.

Barbour A, Philip K. Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.

PLoS One 2014;9(6):e100541. rd




About this article

Title

ANALYSIS OF THE INHIBITION POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM THE SALIVA AND DORSUM OF THE TONGUE OF ADULTS ON THE GROWTH OF STREPTOCOCCUS MUTANS

Keywords

Probiotic, Streptococcus salivarius, Streptococcus mutans.

DOI

10.22159/ajpcr.2017.v10s5.23082

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol. 10, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 11-15

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

80 Views | 58 Downloads

Authors & Affiliations

Deborah Chintyanita
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Indonesia

Hedijanti Joenoes
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Indonesia

Boy M Bachtiar
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Indonesia


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