EFFECTS OF HUMAN PLATELET LYSATES WITHOUT ADDITIONAL GROWTH FACTORS ON THE PROTEIN PROFILES OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELL CULTURE MEDIA


Hazriani Ra, Lisa Amir, Ratna Farida

Abstract


Objectives: The objective of this study is to evaluate the effect of HPLs with no additional GFs on the HUVEC protein profile.

Methods: HUVEC cultures were examined in groups as follows: Fetal bovine serum (FBS), 2%-HPL with a GF, and 2%- and 5%-HPL without a GF which were analyzed with a sodium dodecyl sulfate-polyacrylamide gel electrophoresis test.

Results: The intensity, thickness, and molecular weight of HUVEC band proteins cultured without a GF were not significantly different compared to the control groups (FBS or HPL with a GF).

Conclusions: No difference was found in the HUVEC protein profile after they were cultured with FBS and HPLs, with or without GFs.


Keywords


Fetal bovine serum, Growth factor, Human platelet lysate, Human umbilical vein endothelial cell, Protein profile.

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About this article

Title

EFFECTS OF HUMAN PLATELET LYSATES WITHOUT ADDITIONAL GROWTH FACTORS ON THE PROTEIN PROFILES OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELL CULTURE MEDIA

Keywords

Fetal bovine serum, Growth factor, Human platelet lysate, Human umbilical vein endothelial cell, Protein profile.

DOI

10.22159/ajpcr.2017.v10s5.23095

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Special Issue October 2017 [PTMDS 2017] Page: 54-56

Print ISSN

0974-2441

Online ISSN

2455-3891

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Authors & Affiliations

Hazriani Ra
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia
Indonesia

Lisa Amir
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia
Indonesia

Ratna Farida
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia
Indonesia


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