THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION

  • Sri Angky Soekanto Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Gadiacanaparimita Ghrena Duhita Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Endang Winiati Bachtiar Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Muhamad Sahlan Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.

Abstract

 

Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.

Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.

Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).

Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

Keywords: Caries, Saliva, Casein phosphopeptide-amorphous calcium phosphate, Propolis wax, Biofilm.

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Soekanto, S. A., G. G. Duhita, E. W. Bachtiar, and M. Sahlan. “THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 17, Oct. 2017, pp. 174-6, doi:10.22159/ajpcr.2017.v10s5.23125.
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