ISOLATION AND CHARACTERIZATION OF PHYTOSTEROLS FROM CORDIA MACLEODII (HOOK F. AND THOMSON) BARK BY CHROMATOGRAPHIC AND SPECTROSCOPIC METHOD
Â Aims and objectives: The study focuses on isolation and determination of the chemical constituents of the Cordia macleodii bark, used as medicinal plant in folklore system. The principal theme of the study is to develop applied chromatographic techniques for the separation, isolation and detection of the compounds.
Methods: A petroleum extract of bark were analyzed by GC/MS, IR, and UV. The structures were elucidated on the basis GC-MS library of reported data.
Result: Three known compounds Stigmasterol, Cholest-5-EN -3OL (3 Beta)-Carbonyl chlorinated, Camphesterol were determined from Cordia macleodii bark. These compounds were isolated from this plant for the first time.
Conclusion: From the present study, it is concluded that chromatographic and spectroscopy has potential as rapid and simple tools in the isolation and analysis of various compounds from Cordia macleodii bark.
Keywords: Gas chromatography-mass spectrometry, Infrared, Chromatography, Spectroscopy, Phytosterols, Cordia macleodii
Bennett RD, Heftmann ST. Isolation of estrone and cholesterol from the date palm Phoenix dactylifera. Phytochemistry 1966;5(2):231.
Dean PD, Exley D, Goodwin TW. Steroid estrogens in plants: Re-estimation of oestrone in pomegranate seeds. Phytochemistry 1971;10:2215-6.
Nakanishi K, Karieda M, Sasaki S, Chang ML. 5-Î²-Hydroxy-ecdysones and a revision of the structure of ponasterone C. Chem Commum 1966;915; doi: 10.1039/C29700000351.
Vohora SB, Wani H. A review of Indian anti-inflammatory plants 1947-1982. Herba Hungarica 1987;26(1):73-9.
Vohara SB, Khan MS. Antifertility studies on Unani herbs, Part III. Antiovulatory effects of two plant saponins. Indian J Pharm 1974;36:77.
Kochhar SP. Influence of processing on sterols of edible vegetable oils. Prog Lipid Res 1983;22(3):161-88.
Johansson-Kornfelt A. Sterols in vegetable oils. Doctoral Thesis. Uppsala: Swedish University of Agricultural Sciences; 1979.
Clark J. Tococpherols and sterols from soyabeans. Lipid Technol 1996;5:111.
Pollak OJ, Kritchevsky D. Sitosterol. Monogr Atheroscler 1981;10:1-219.
Miettinen TA, Gylling H. In: Yalpani M, editors. New Technologies for Healthy Foods and Nutraceuticals. Shrewsbury: ATL Press; 1997. p. 71.
Weststrate JA, Meijer GW. Plant sterol-enriched margarines and reduction of plasma total- and LDL-cholesterol concentrations in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur J Clin Nutr 1998;52:334-43.
Law M. Plant sterol and stanol margarines and health. BMJ 2000;320(7238):861-4.
Nadkarni KM. The Indian Materia Medica. Bombay: Popular Prakashan Private Limited; 1976. p. 380.
Mangold HK. In: Stalh E, editors. Thin Layer Chromatography. New York: Springer-Verlag; 1969. p. 371.
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