ASSESSMENT OF PHYSICOCHEMICAL PROPERTIES AND PHYTOCHEMICAL CONSTITUENTS ALONG WITH ANTIBACTERIAL POTENTIAL OF TARENNA POLYCARPA LEAF FRACTIONS
Keywords:Tarenna polycarpa, Fractions, Antimicrobial, Phytochemical, Physicochemical, Agar well diffusion method
Â Objective: The aim was to investigate the physicochemical analysis, preliminary phytochemical screening and further evaluation of its antibacterial activity from morbesi-besi (Tarenna polycarpa) leaf ethyl acetate and hexane fractions (HF).
Methods: The physicochemical parameters were carried out as per the World Health Organization (WHO) guideline from its ethanolic extract. The phytochemical screening was performed as per Indonesia Pharmacopoeia and conventional method on its ethyl acetate and HF. Ethyl acetate and HF at different concentration were evaluated using agar well diffusion assay.
Result: The plant was found to be free from contaminations. Morbesi-besi leaf fractions are composed of flavonoids, glycosides, saponins, tannins, steroids, and triterpenoids. The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The antibacterials assay showed that ethyl acetate fraction was better than HF from the inhibition zone.
Conclusion: From the outcomes of this study, morbesi-besi leaf (T. polycarpa) possess potential antibacterial activity.
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