IN VITRO CYTOTOXIC ANTICANCER POTENTIAL OF BIOACTIVE FRACTION ISOLATED FROM INDONESIAN TIDAL SPONGE CALTHROPELLA SP.

  • Fitria Susilowati Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,
  • Respati Tri Swasono Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,
  • Tatsufumi Okino Department of Graduate School of Environmental Sciences, Hokkaido University, Sapporo, Japan.
  • Winarto Haryadi Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,

Abstract

Objective: This study was taken to examine the cytotoxicity of the bioactive fraction isolated from marine sponge Calthropella sp. as a preliminary anticancer assay and identify its bioactive compounds.

Methods: The cytotoxic activity was assessed by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay against three human cancer cell lines, namely human breast (MCF-7), human lung (H-460), and human liver (HepG-2). The bioactive compounds were identified using a high-resolution liquid chromatography–mass spectroscopy (LC–MS).

Results: The active fraction 7 showed moderate to strong cytotoxic activity on all cell lines tested and promising a strong potent cytotoxicity against MCF-7 cell lines with IC50 value as low as 1.925 μg/mL comparable to control, cisplatin (IC50 0.977 μg/mL). In regard to the promising bioactive compounds, the high-resolution LC–MS predicted the existing of several known compounds such as bengamide Q, clavepictine A, 4’-N-methyl-5’- hydroxystaurosporine, carteriofenone A, and one strong possibility of a new compound.

Conclusion: This study has revealed that the isolated bioactive fraction of Indonesian tidal sponge, Calthropella sp., possesses potential anticancer properties with a promising significant cytotoxicity on MCF-7 cell lines (IC50 1.925 μg/mL).

Keywords: Calthropella sp., 3-(4, 5-Dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide assay, MCF-7, H-460, HepG-2.

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How to Cite
Susilowati, F., R. Tri Swasono, T. Okino, and W. Haryadi. “IN VITRO CYTOTOXIC ANTICANCER POTENTIAL OF BIOACTIVE FRACTION ISOLATED FROM INDONESIAN TIDAL SPONGE CALTHROPELLA SP.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 380-3, doi:10.22159/ajpcr.2019.v12i1.23655.
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