• Fitria Susilowati Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,
  • Respati Tri Swasono Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,
  • Tatsufumi Okino Department of Graduate School of Environmental Sciences, Hokkaido University, Sapporo, Japan.
  • Winarto Haryadi Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia,


Objective: This study was taken to examine the cytotoxicity of the bioactive fraction isolated from marine sponge Calthropella sp. as a preliminary anticancer assay and identify its bioactive compounds.

Methods: The cytotoxic activity was assessed by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay against three human cancer cell lines, namely human breast (MCF-7), human lung (H-460), and human liver (HepG-2). The bioactive compounds were identified using a high-resolution liquid chromatography–mass spectroscopy (LC–MS).

Results: The active fraction 7 showed moderate to strong cytotoxic activity on all cell lines tested and promising a strong potent cytotoxicity against MCF-7 cell lines with IC50 value as low as 1.925 μg/mL comparable to control, cisplatin (IC50 0.977 μg/mL). In regard to the promising bioactive compounds, the high-resolution LC–MS predicted the existing of several known compounds such as bengamide Q, clavepictine A, 4’-N-methyl-5’- hydroxystaurosporine, carteriofenone A, and one strong possibility of a new compound.

Conclusion: This study has revealed that the isolated bioactive fraction of Indonesian tidal sponge, Calthropella sp., possesses potential anticancer properties with a promising significant cytotoxicity on MCF-7 cell lines (IC50 1.925 μg/mL).

Keywords: Calthropella sp., 3-(4, 5-Dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide assay, MCF-7, H-460, HepG-2.


1. World Health Organization. Global Status Report on Noncommunicable Diseases. Switzerland: WHO Press; 2011. p. 176.
2. Indonesian Ministry of Health. Riset Kesehatan Dasar 2013 Badan Litbangkes Dan Data Penduduk Sasaran. Jakarta: Pusdatin Kementerian Kesehatan RI; 2014. p. 306.
3. Florea AM, Büsselberg D. Cisplatin as an anti-tumor drug: Cellular mechanisms of activity, drug resistance and induced side effects. Cancers (Basel) 2011;3:1351-71.
4. Blunt JW, Copp BR, Hu WP, Munro MH, Northcote PT, Prinsep MR, et al. Marine natural products. Nat Prod Rep 2009;26:170-244.
5. Simmons TL, Andrianasolo E, McPhail K, Flatt P, Gerwick WH. Marine natural products as anticancer drugs. Mol Cancer Ther 2005;4:333-42.
6. Molinski TF, Dalisay DS, Lievens SL, Saludes JP. Drug development from marine natural products. Nat Rev Drug Discov 2009;8:69-85.
7. Mehbub MF, Lei J, Franco C, Zhang W. Marine sponge derived natural products between 2001 and 2010: Trends and opportunities for discovery of bioactives. Mar Drugs 2014;12:4539-77.
8. Graham-Evans B, Tchounwou PB, Cohly HH. Cytotoxicity and proliferation studies with arsenic in established human cell lines: keratinocytes, melanocytes, dendritic cells, dermal fibroblasts, microvascular endothelial cells, monocytes and tcells. Int J Mol Sci 2003;4:13-21.
9. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 1988;48:589-601.
10. Susilawati S, Sabirin M, Harno DP, Chairil A. Antioxidant activity of 2,6,4’-trihydroxy-4-methoxy benzophenone from ethyl acetate extract of leaves of mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.). Indo J Chem 2011;11:180-5.
11. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
12. Lit M. A Database of the Marine Natural Product Literature. Royal Society of Chemistry (UK). Available from: marinlit/. [Last accessed on 2015 Nov ???].
13. White KN, Tenney K, Crews P. The bengamides: A Mini-review of natural sources, analogues, biological properties, biosynthetic origins, and future prospects. J Nat Prod 2017;80:740-55.
14. Hernández LM, Blanco JA, Baz JP, Puentes JL, Millán FR, Vázquez FE, et al. 4’-N-methyl-5’-hydroxystaurosporine and 5’-hydroxystaurosporine, new indolocarbazole alkaloids from a marine Micromonospora sp. Strain. J Antibiot (Tokyo) 2000;53:895-902.
15. Raub MF, Cardellina JH 2nd, Choudhary MI, Ni CZ, Clardy J, Alley MC. Clavepictines A and B: Cytotoxic quinolizidines from the tunicate Clavelina picta. J Am Chem Soc 1991;113:3178.
16. Habli Z, Toumieh G, Fatfat M, Rahal ON, Gali-Muhtasib H. Emerging cytotoxic alkaloids in the battle against cancer: Overview of molecular mechanisms. Molecules 2017;22. pii: E250.
17. Ardiansah B. Recent reports on pyrazole-based bioactive compounds as candidate for anticancer agents. Asian J Pharm Clin Res 2017;10:45-51.
18. Ismail MM, Soliman DH, Farrag AM, Sabour R. Synthesis, antitumor activity, pharmacophore modelling and QSAR studies of novel pyrazolesand pyrazolo [1,5-a] pyrimidines against breast adenocarcinoma MCF-7 cell line. Int J Pharm Pharm Sci 2016;8:434-42.
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How to Cite
Susilowati, F., R. Tri Swasono, T. Okino, and W. Haryadi. “IN VITRO CYTOTOXIC ANTICANCER POTENTIAL OF BIOACTIVE FRACTION ISOLATED FROM INDONESIAN TIDAL SPONGE CALTHROPELLA SP.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 380-3, doi:10.22159/ajpcr.2019.v12i1.23655.
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