PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS AND COAGULASE-NEGATIVE STAPHYLOCOCCI IN A TERTIARY CARE HOSPITAL.

  • BILAL AHMAD MIR Microbiology Khaja Banda Nawaz institute of medical sciences, Gulbarga
  • Srikanth . khaja Banda Nawaz institute of medical sciences

Abstract

Background: Globally nosocomial infection is a major problem. Prevalence and antibiotic resistance of methicillin-resistant Staphylococcus aureus (MRSA) strains is reported to be increasing globally. MRSA and methicillin resistant coagulase negative staphylococci (MRCoNS) are the important agents causing nosocomial infections.

Objective: The study was conducted to find out the prevalence rate of MRSA and MRCoNS and antibiotic susceptibility pattern.

Materials and Methods: This was a retrospective study conducted from June 2011 to November 2012 in a tertiary care hospital in south India. All isolates were identified by Clinical and Laboratory Standards Institute (CLSI) guidelines and antibiotic susceptibility pattern determined by modified Kirby Bauer disc diffusion method. The information was recorded and analyzed using Microsoft Excel (2007 version).

Results: A total of 210 Staphylococcus strains were isolated from various clinical samples, 180 were coagulase positive staphylococcus (CoPS) and 30 were coagulase negative staphylococcus (CoNS). Among 180 CoPS, 58 (32.22%) were Methicillin resistant and among CoNS, 12 (40%) were methicillin resistant. In MRSA maximum resistance was seen with oxacillin (93.2%) and least with vancomycin (3.5%). In MRCoNS maximum resistance was seen with oxacillin (91.7%) and least with vancomycin (0 %).

Conclusion: There is need for continuous monitoring of the antimicrobial susceptibility pattern of methicillin staphylococcus aureus and methicillin resistant coagulase negative staphylococci for the selection of appropriate therapy, developing the antibiotic policy and for limiting the use of powerful antibiotics.

Key words: Methicillin-resistant Staphylococcus aureus (MRSA), Methicillin resistant coagulase negative staphylococci, Vancomycin, Nosocomial infection, Susceptibility pattern

Author Biographies

BILAL AHMAD MIR, Microbiology Khaja Banda Nawaz institute of medical sciences, Gulbarga
Tutor, Department of Microbiology
Srikanth ., khaja Banda Nawaz institute of medical sciences
Assistant professor, Department of Pharmacology

References

1) Tiwari HK, Das AK, Sapkota D, Sivarajan K, Pahwa VK. Methicillin resistant Staphylococcus aureus: Prevalence and antibiogram in a tertiary care hospital in western Nepal. J Infect Dev Ctries 2009;3:681-4.

2) Al-Baidani AH, El-Shouny WH, Shawa TM. Antibiotic Suseptiblity Pattern of Methicillin Resistance Staphylococcus aureus in Three Hospitals at Hodeidah City, Yemen. Global J. Pharmacol. 2011;5(2):106-111.

3) Maple PAC, Hamilton- Miller JMT, Brumfitt W. Worldwide antibiotic resistance in methicillin resistant Staphylococcus aureus. Lancet 1989;1:537-40.

4) Patel AK, Patel KK, Patel KR, Shah S, Dileep P. Time trends in the epidemiology of microbial infections at a tertiary care center in west India over last 5 years. J Assoc Physicians India 2010;58:37-40.

5) Gopalakrishnan R, Sureshkumar D. Changing trends in antimicrobial susceptibility and hospital acquired infections over an 8 year period in a tertiary care hospital in relation to introduction of an infection control programme. J Assoc Physicians India 2010;58:25-31.

6) Assadullah S, Kakru DK, Thoker MA, Bhat FA, Hussain N, Shah A. Emergence of low level Vancomycin resistance in MRSA. Indian J Med Microbiol 2003;21:196-8.

7) McDonald M - The epidemiology of methicillin resistant Staphylococcus aureus: Surgical relevance 20 years on. Aust N Z J Surg 1997;67:682-5.

8) Performance Standards for Antimicrobial Susceptibility Testing; 20th Informational Supplement, Clinical and Laboratory Standards Institute (CLSI) M100-S20: Vol. 30, No.1. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.

9) Mulla S, Patel M, Shah L, Vaghela G. Study of antibiotic sensitivity pattern of methicillin-resistant Staphylococcus aureus. Indian J Critical Care Medicine. 2007;11(2):99-101.

10) Rajaduraipandi K, Mani KR, Panneerselvam K, M, Bhaskar M, Manikandan P. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus: a multicentre study. Indian J Med Microbiol 2006;24:34-8.

11) Tiwari HK, Sapkota D, Sen MR. High prevalence of multidrug-resistant MRSA in a tertiary care hospital of northern India. Infection and Drug Resistance 2008;1:57-61.

12) Sangeeta Joshi, Pallab Ray, Vikas Manchanda, Jyoti Bajaj, D.S. Chitnis, Vikas Gautam. Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence & susceptibility pattern. Indian J Med Res 2013;137(2):363–369.

13) Dar JA, Thoker MA, Khan JA, Ali A, Khan MA, Rizwan M, et al. Molecular epidemiology of clinical and carrier strains of methicillin resistant Staphylococcus aureus (MRSA) in the hospital settings of north India. Ann Clin Microbiol Antimicrob 2006;5:22.

14) S Anupurba, MR Sen, G Nath, BM Sharma, AK Gulati, TM Mohapatra. Prevalence of methicillin resistant Staphylococcus aureus in a tertiary referral hospital in eastern Uttar Pradesh. Ind. J. Med. Microbiol 2003;21(1):49-51.

15) Vidya Pai, Venkatakrishna I Rao, Sunil P Rao. Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus Aureus [MRSA] Isolates at a Tertiary Care Hospital in Mangalore, South India. Journal of Laboratory Physicians 2010;2(2):82-4.

16) Arora S, Devi P, Arora U and Devi V. Prevalence of methicillin resistance staphylococcus aureus in a tertiary care hospital in Northern India. Journal of Lab Physicians 2010;2(2):78-81.

17) Saikia L, Nath R Choudhary B, Sarkar M. Prevalence and antimicrobial susceptibility pattern of methicillinresistant Staphylococcus aureus in Assam. Indian J Crit Care Med 2009;13:156-8.

18) Kumari N, Mohapatra TM, Singh YI. Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Tertiary-Care Hospital in Eastern Nepal. J Nepal Med Assoc 2008;47(170):53-6.

19) Tsering DC, Pal R, Kar S. Methicillin-resistant Staphylococcus Aureus:Prevalence and current susceptibility pattern in Sikkim. J Global Infect Dis 2011;3:9-13.

20) Menezes GA, Harish BN, Sujatha S, Vinothini K, Parija SC. Emergence of vancomycin-intermediate Staphylococcus species in southern India. J Med Microbiol 2008;57:911-2.

21) Tiwari HK, Sen MR. Emergence of vancomycin resistant Staphylococcus aureus (VRSA) from a tertiary care hospital from northern part of India.BMC Infect Dis 2006;6:156

22) Sohn A, Garrett D, Sinkowitz-Cochran R. Prevalence of nosocomial infections in neonatal intensive care unit patients: results from the first national point-prevalence survey. J Pediatr 2001;136:821-27.

23) Stoll B, Gordon J. Late-onset sepsis in very low birth weight neonates: a report from the National Institute of Child Health and Human Development Neonatal Research Network. J Pediat 1996;129:63-71.

24) Koksal F, Yasar H, Samasti M. Antibiotics resistant patterns of coagulase-negative staphylococcus strains from blood cultures of septicemic in Turkey Microbiol Res 2007;16:31-4.

25) Dr. E. Shamsadh Begum, Dr. N. Anbumani, Dr. J. Kalyani, Dr. M. Mallika. Prevalence and antimicrobial susceptibility pattern of Coagulase-negative Staphylococcus. International Journal of Medicine and Public Health 2011;1(4):59-62.

26) Irum Perveen, Abdul Majid, Sobia Knawal, Iffat Naz, Shama Sehar, Safia Ahmed et.al. Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococci in Rawalpindi, Pakistan. Br J Med Med Res, 2013;3(1):198-209.

27) Sanyal D , Johnson AP , George RC , Edwards R , Greenwood D In-vitro characteristics of glycopeptide resistant strains of Staphylococcus epidermidis isolated from patients on CAPD. J Antimicrob Chemother 1993;32 (2):267-278.

28) Worthington T , Lambert PA , Elliot TS . Is hospital-acquired intravascular catheter-related sepsis associated with outbreak strains of coagulasenegative Staphylococci? J Hos Infect 2000; 46:130-34.

29) De Paulis A , Predari S , Chazarreta C , Santoiani J . Five-test simple scheme for species level identification of clinically significant Coagulase Negative Staphylococci. J Clin Microbiol 2003;41:1219-24.

30) Sanzen L, Walder M . Antibiotic resistance of Coagulase negative Staphylococci in an orthopaedic department. J Hosp Infect 1988;12:103-108.

31) Christensen GD . The sticky problem of Staphylococcus epidermidis sepsis. Hosp Pract 1993;30:27-38.

32) Pfaller MA , Herwaldt LA . Laboratory, Clinical, and Epidemiological Aspects of Coagulase Negative Staphylococci. Clin Microbiol Rev 1988;1:281–299.
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How to Cite
MIR, B. A., and S. . “PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS AND COAGULASE-NEGATIVE STAPHYLOCOCCI IN A TERTIARY CARE HOSPITAL.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 6, no. 7, Aug. 2013, pp. 231-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/237.
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