ANTIMUTAGENIC ACTIVITY OF BINARA HERBS (ARTEMISIA VULGARIS L.) ETHANOL EXTRACT IN MALE MICE INDUCED BY CYCLOPHOSPHAMIDE


Linta Meliala, Urip Harahap, Urip Harahap, Aminah Dalimunthe, Aminah Dalimunthe

Abstract


 Objectives: This study aims to evaluate the antimutagenic activity of binara herbs (Artemisia vulgaris L.) ethanol extract on male mice.

Methods: Binara herbs powder was extracted is used ethanol 96% by maceration, then made into a suspension preparation used 0.5% carboxymethylcellulose with 3 dose variations (100, 200, and 300 mg/kg). Further, the extract was administrated orally in mice for 7 consecutive days. 4 h after the last orally, cyclophosphamide 50 mg/kg IP was injected. 30 h after cyclophosphamide injection, the animals were killed, and the samples of bone marrow were prepared and stained with gems. For each sample, 200 cells of polychromatic erythrocytes (PCE) and the same number of normochromatic erythrocytes (NCE) and the cells containing their micronucleus (MN) were counted using the MN test method in vivo.

Results: Cyclophosphamide increased the frequency of MnPCE and decreased cell proliferation (PCE/PCE+NCE). All doses of extracts significantly reduced the frequency of MnPCE (p<0.05). The cell proliferation ratio (PCE/PCE+NCE) was also increased. The most effective dose is 300 mg/kg because it has the greatest ability to decrease the frequency of MnPCE and increase the cell proliferation ratio.

Conclusion: Results of this study indicated that binara herbs (A. vulgaris L.) ethanol extract has potent antimutagenic activity.


Keywords


Binara herbs, Antimutagenic, Micronucleus test.

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About this article

Title

ANTIMUTAGENIC ACTIVITY OF BINARA HERBS (ARTEMISIA VULGARIS L.) ETHANOL EXTRACT IN MALE MICE INDUCED BY CYCLOPHOSPHAMIDE

Keywords

Binara herbs, Antimutagenic, Micronucleus test.

DOI

10.22159/ajpcr.2018.v11i4.24176

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 236-238

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

34 Views | 72 Downloads

Authors & Affiliations

Linta Meliala
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Indonesia

Urip Harahap
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Indonesia

Urip Harahap
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Indonesia

Aminah Dalimunthe
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Indonesia

Aminah Dalimunthe
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Indonesia


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