• Kamadyaapa Davie Rexon Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.
  • Gondwe Mavuto Masopera Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.
  • Shauli Mathulo Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.
  • Sewani Rusike Constance Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.
  • Nkeh Chungag Benedicta Department of Biological Sciences, Faculty of Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.



 Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).

Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.

Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.

Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

Keywords: Erythrina abbysinica, antidiabetic, antioxidant, acute, sub-chronic, brine shrimp.

Author Biography

Kamadyaapa Davie Rexon, Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha 5117, Republic of South Africa.


1. Loots DT, Pieters M, Islam MS, Botes L. Antidiabetic effects of Aloe ferox and Aloe greatheadii var. davyana leaf gel extracts in a low-dose streptozotocin diabetes rat model. South Afr J Sci 2011;107:46-51. 2. Kumar V, Abbas AK, Aster JC. Robbins Basic Pathology E-Book: Elsevier Health Sciences; 2017. 3. Aguiree F, Brown A, Cho NH, Dahlquist G, Dodd S, Dunning T, et al. IDF Diabetes Atlas. Vol. 4; 2013. 4. Hall V, Thomsen RW, Henriksen O, Lohse N. Diabetes in Sub Saharan Africa 1999-2011: Epidemiology and public health implications. A systematic review. BMC Public Health 2011;11:564.
5. Mayosi BM, Lawn JE, Van Niekerk A, Bradshaw D, Karim SS, Coovadia HM, et al. Health in South Africa: Changes and challenges since 2009. Lancet 2012;380:2029-43.
6. Musabayane CT. The effects of medicinal plants on renal function and blood pressure in diabetes mellitus. Cardiovasc J Afr 2012;23:462-8.
7. Ullah R, Tariq SA, Khan N, Sharif N, Din ZU, Mansoor K. Antihyperglycemic effect of methanol extract of Tamarix aphylla L.
Karst (Saltcedar) in streptozocin-nicotinamide induced diabetic rats. Asian Pac J Trop Biomed 2017;7:619-23.
8. Zhang F, Lavan BE, Gregoire FM. Selective modulators of PPAR-γ activity: Molecular aspects related to obesity and side-effects. PPAR Res 2007;2007:32696.
9. Mohammed A, Ibrahim MA, Islam MS. African medicinal plants with antidiabetic potentials: A review. Planta Med 2014;80:354-77.
10. Campbell RK, White JR Jr., Saulie BA. Metformin: A new oral biguanide. Clin Ther 1996;18:360-71.
11. DiMatteo MR. Social support and patient adherence to medical treatment: A meta-analysis. Health Psychol 2004;23:207-18.
12. Simmonds MS, Howes MJ. Plants used in the treatment of diabetes. Traditional medicine for modern times-Antidiabetic Plants. Vol. 6. New York: CRC Press; 2006. p. 19-82.
13. Haque N, Salma U, Nurunnabi TR, Uddin MJ, Jahangir MF, Islam SM, et al. Management of type 2 diabetes mellitus by lifestyle, diet and medicinal plants. Pak J Biol Sci 2011;14:13-24.
14. Malviya N, Jain S, Malviya SA. Antidiabetic potential of medicinal plants. Acta Pol Pharm 2010;67:113-8.
15. Qi LW, Liu EH, Chu C, Peng YB, Cai HX, Li P. Anti-diabetic agents from natural products-an update from 2004 to 2009. Curr Top Med Chem 2010;10:434-57.
16. Bnouham M, Ziyyat A, Mekhfi H, Tahri A, Legssyer A. Medicinal plants with potential antidiabetic activity-A review of ten years of herbal medicine research (1990-2000). Int J Diabetes Metab 2006;14:1.
17. Kazembe T, Chikuyu J. In vitro efficacy of extracts of Cantharanthus roseus and Erythrina abbysinica on Babesia bigemina. Bull Environ Pharmacol Life Sci 2012;1:32-38.
18. Kareru PG, Kenji GM, Gachanja AN, Keriko JM, Mungai G. Traditional medicines among the Embu and Mbeere people of Kenya. Afri J Tradit Complement Altern Med 2007;4:75-86.
19. Hines DA, Eckman K. Indigenous Multipurpose Trees of Tanzania: Uses and Economic Benefits for People. Rome: FAO; 1993.
20. Piero NM, Joan MN, Kibiti CM, Ngeranwa J, Njue WN, Maina DN, et al. Hypoglycemic activity of some Kenyan plants traditionally used to manage diabetes mellitus in Eastern Province. J Diabetes Metab 2011;2:155.
21. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010;107:1058-70.
22. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DJ, McLaughlin JL. Brine shrimp: Aconvenient general bioassay for active plant constituents. Planta Med 1982;45:31-4.
23. Nguta JM, Mbaria JM, Gakuya DW, Gathumbi PK, Kabasa JD, Kiama SG. Evaluation of acute toxicity of crude plant extracts from kenyan biodiversity using brine shrimp, Artemia salina L. (Artemiidae). In Open Conf Proc J 2012;3:30-4.
24. Bastos ML, Lima MR, Conserva LM, Andrade VS, Rocha EM, Lemos RP. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microbiol Antimicrob 2009;8:16.
25. Olurishe CO, Salawu OA, Zezi AU, Olurishe TO, Bisalla M. Metformin-cefixime co-administration affects glucose regulation and reno-pancreatic histology in alloxan-induced hyperglycemic rats. J Pharm Sci Tech 2013;3:43-50.
26. Turkoglu A, Duru ME, Mercan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem 2007;101:267-73.
27. Nair VD, Dairam A, Agbonon A, Arnason JT, Foster BC, Kanfer I, et al. Investigation of the antioxidant activity of African potato (Hypoxis hemerocallidea). J Agric Food Chem 2007;55:1707-11.
28. Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K. Methods for testing antioxidant activity. Analyst 2002;127:183-98.
29. Gohari AR, Hajimehdipoor H, Saeidnia S, Ajani Y, Hadjiakhoondi A. Antioxidant activity of some medicinal species using FRAP assay. J Med Plants 2011;1:54-60.
30. Moukette BM, Pieme CA, Njimou JR, Biapa CP, Marco B, Ngogang JY, et al. In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res 2015;48:15.
31. Cham A, Cham K, Chase T, Cham BE. A standardized plant extract containing a target compound is acceptable as a potent therapeutic entity: Relevance to BEC and solamargine, a topical clinical formulation curaderm BEC5. J Cancer Treat Res 2015;3:22-7.
32. Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK, et al. Antidiabetic agents from medicinal plants. Curr Med Chem 2006;13:1203-18.
33. Basal N. Prediabetes diagnosis and treatment. World J Diabetes 2015;6:296-303.
34. Brogan IJ, Murray IA, Cerillo G, Needham M, White A, Davis JR. Interaction of glucocorticoid receptor isoforms with transcription factors AP-1 and NF-κB: Lack of effect of glucocorticoid receptor β. Mol Cell Endocrinol 1999;157:95-104.
35. Khavandi K, Amer H, Ibrahim B, Brownrigg J. Strategies for preventing type 2 diabetes: An update for clinicians. Ther Adv Chronic Dis 2013;4:242-61.
36. Oguanobi NI, Chijioke CP, Ghasi SI. Effects of aqueous leaf extract of Ocimum gratissimum on oral glucose tolerance test in type-2 model diabetic rats. Afri J Pharm Pharmacol 2012;6:630-5.
37. Kavitha S, Rajeshwari S, Ravid D. Antidiabetic activity of Asystasia gangetica (L.) T. Anderson flower extract in streptozotocin induced diabetic rats. Int J Pharm Pharm Sci 2016;8:79-84.
38. Deshmukh CD, Jain A. Antidiabetic and antihyperlipidemic effects of methanolic extract of Citrullus lanatus seeds in rats. Int J Pharm Pharm Sci 2015;7:232-6.
39. Patel DK, Prasad SK, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012;2:320-30.
40. Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. Increased oxidative stress in rat liver and pancreas during progression of streptozotocin-induced diabetes. Clin Sci (Lond) 1998;94:623-32.
41. Eleazu CO, Okafor P. Use of unripe plantain (Musa paradisiaca) in the management of diabetes and hepatic dysfunction in streptozotocin induced diabetes in rats. Interv Med Appl Sci 2015;7:9-16.
42. Gondwe M, Kamadyaapa DR, Tufts M, Chuturgoon AA, MusabayaneCT. Sclerocarya birrea [(A. Rich.) hochst.] [Anacardiaceae] stem-bark ethanolic extract (SBE) modulates blood glucose, glomerular filtration rate (GFR) and mean arterial blood pressure (MAP) of STZ-induced diabetic rats. Phytomedicine 2008;15:699-709.
43. Moodley K, Joseph K, Naidoo Y, Islam S, Mackraj I. Antioxidant, antidiabetic and hypolipidemic effects of Tulbaghia violacea harv. (wild garlic) rhizome methanolic extract in a diabetic rat model. BMC Complement Altern Med 2015;15:408.
44. Shenoy AG, Goyal RK. Improvement of insulin sensitivity by perindopril in spontaneously hypertensive and streptozotocin-diabetic rats. Indian J Pharmacol 2002;34:156-64.
45. Eleazu CO, Eleazu KC. Ameliorating potentials of 3 medicinal plants on relative pancreatic weights in streptozotocin induced diabetic rats. J Diabetes Metab 2013;4:2.
46. Brownlee M. The pathobiology of diabetic complications. Diabetes 2005;54:1615-25.
47. Gultekin F, Doguc DK. Allergic and immunologic reactions to food additives. Clin Rev Allergy Immunol 2013;45:6-29.
48. Rani JS. The efficacy of aqueous extract of Talinum triangulare on carbohydrate metabolic, non-enzymatic antioxidants and antioxidantive enzymes in alloxan induced diabetic rats. Arch Appl Sci Res 2013;5:197202.
49. Olorunnisola OS, Bradley G, Afolayan AJ. Protective effect of T. Violacea rhizome extract against hypercholesterolemia-induced oxidative stress in wistar rats. Molecules 2012;17:6033-45.
50. Hamidi M, Jovanova B, Panovska T. Toxicоlogical evaluation of the plant products using brine shrimp (Artemia salina L.) model. Macedonian Pharm Bull 2014;60:9-18.
51. Naidu JR, Ismail R, Sasidharan S. Acute oral toxicity and brine shrimp lethality of methanol extract of Mentha Spicata L (Lamiaceae). Trop J Pharm Res 2014;13:101-7.
52. Kheiri Manjili H, Jafari H, Ramazani A, Davoudi N. Anti-leishmanial and toxicity activities of some selected Iranian medicinal plants. Parasitol Res 2012;111:2115-21.
53. Costa-Lotufo LV, Khan MT, Ather A, Wilke DV, Jimenez PC, Pessoa C, et al. Studies of the anticancer potential of plants used in Bangladeshi folk medicine. J Ethnopharmacol 2005;99:21-30.
54. Arslanyolu M, Erdemgil FZ. Evaluation of the antibacterial activity and toxicity of isolated arctiin from the seeds of Centaurea sclerolepis. Ankara Ecz Fak Derg 2006;35:103-9.
55. Yenesew A, Induli M, Derese S, Midiwo JO, Heydenreich M, Peter MG, et al. Anti-plasmodial flavonoids from the stem bark of Erythrina abyssinica. Phytochemistry 2004;65:3029-32.
56. Piero NM, Joan MN, Cromwell KM, Joseph NJ, Wilson NM, Peter KG, et al. Hypoglycemic activity of some kenyan plants traditionally used to manage diabetes mellitus in eastern province. J Diabetes Metab 2011;2:155.
57. Maroyi A. Traditional use of medicinal plants in south-central Zimbabwe: Review and perspectives. J Ethnobiol Ethnomed 2013;9:31.
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How to Cite
Davie Rexon, K., G. Mavuto Masopera, S. Mathulo, S. R. Constance, and N. C. Benedicta. “EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 300-6, doi:10.22159/ajpcr.2018.v11i8.24207.
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