HISTOPATHOLOGICAL LIVER TISSUES OF ALLOXAN-INDUCED WISTAR RATS THAT GIVEN LAWSONIA INERMIS (LINN.) LEAVES' ETHANOLIC EXTRACT

  • Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.
  • Dwi Rita Anggraini Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.

Abstract

Objectives: The aim of this research was to see the effect of Lawsonia inermis (Linn.) leaves ethanolic extract (LLEE) on liver histopathological of alloxan-induced Wistar rats.

Methods: Thirty-five of male Wistar rats grouped into five groups, i.e., K (normal) given 0.9% NaCl solution and P1–P4 were induced using alloxan (120 mg/kg BW) intraperitoneally. P2–P4 was given LLEE, i.e., 200 mg/kg BW, 400 mg/kg BW, and 600 mg/kg BW for 28 days. On 29th day, all Wistar rats of the group were sacrificed to take its liver. Histology preparation of liver by paraffin slicing method of hematoxylin-eosin staining. The observation was done under a microscope with ×40 and ×100 magnification. Observation of the liver histopathological includes changes of the central venous, sinusoid, hepatocyte morphology, and glycogen accumulation, P1–P4 compared to K.

Result: The results showed that in P4 group, LLEE dose 600 mg/Kg BW for 28 days able to improve liver tissues structure of alloxan-induced Wistar rats.

Conclusion: The LLEE at dose 600 mg/Kg BW effective can restore the liver destruction of alloxan-induced Wistar rats.

Keywords: Lawsonia inermis (Linn.), Histopathological, Liver.

Author Biography

Mutiara Indah Sari, Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.
BIOCHEMISTRY UNIVERSITAS SUMATERA UTARA

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Sari, M. I., and D. R. Anggraini. “HISTOPATHOLOGICAL LIVER TISSUES OF ALLOXAN-INDUCED WISTAR RATS THAT GIVEN LAWSONIA INERMIS (LINN.) LEAVES’ ETHANOLIC EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 162-5, doi:10.22159/ajpcr.2018.v11i8.25358.
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