• Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.
  • Dwi Rita Anggraini Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.




Lawsonia inermis (Linn), Histopathological, Liver


Objectives: The aim of this research was to see the effect of Lawsonia inermis (Linn.) leaves ethanolic extract (LLEE) on liver histopathological of alloxan-induced Wistar rats.

Methods: Thirty-five of male Wistar rats grouped into five groups, i.e., K (normal) given 0.9% NaCl solution and P1–P4 were induced using alloxan (120 mg/kg BW) intraperitoneally. P2–P4 was given LLEE, i.e., 200 mg/kg BW, 400 mg/kg BW, and 600 mg/kg BW for 28 days. On 29th day, all Wistar rats of the group were sacrificed to take its liver. Histology preparation of liver by paraffin slicing method of hematoxylin-eosin staining. The observation was done under a microscope with ×40 and ×100 magnification. Observation of the liver histopathological includes changes of the central venous, sinusoid, hepatocyte morphology, and glycogen accumulation, P1–P4 compared to K.

Result: The results showed that in P4 group, LLEE dose 600 mg/Kg BW for 28 days able to improve liver tissues structure of alloxan-induced Wistar rats.

Conclusion: The LLEE at dose 600 mg/Kg BW effective can restore the liver destruction of alloxan-induced Wistar rats.


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Author Biography

Mutiara Indah Sari, Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia.



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How to Cite

Sari, M. I., and D. R. Anggraini. “HISTOPATHOLOGICAL LIVER TISSUES OF ALLOXAN-INDUCED WISTAR RATS THAT GIVEN LAWSONIA INERMIS (LINN.) LEAVES’ ETHANOLIC EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 8, Aug. 2018, pp. 162-5, doi:10.22159/ajpcr.2018.v11i8.25358.



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