EVALUATION OF IN VITRO ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF SEAGRASS HALOPHILA BECCARII

  • Vani M Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
  • Vasavi T Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
  • Uma Maheswari Devi P Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.

Abstract

Objectives: The objectives of this study were to analyze the in vitro antidiabetic activity of methanolic extract of seagrass.

Methods: The methanolic extract of Halophila beccarii was tested for its in vitro antidiabetic potential through inhibition of carbohydrate digestive enzymes such as α- amylase and α-glucosidase, glucose diffusion assay, glucose adsorption capacity, and enhancement of glucose uptake activity in yeast cells.

Results: The crude extract of H. beccarii showed strong inhibition of É‘-amylase and É‘-glucosidase as on comparison with the standard enzyme inhibitor acarbose. The antidiabetic activity was found to be concentration dependent, and the antihyperglycemic activity was demonstrated through the inhibition of the glucose movement and facilitated diffusion of glucose. The rate of glucose diffusion across the membrane was found to be increased from 30 to 180 min.

Conclusion: The seagrass collected from Pulicat Lake was identified as H. beccarii based on the morphology of the plant, shape of the leaves, and brown pigmentation on the leaves. According to the results, H. beccarii showed antidiabetic activity by the inhibition of carbohydrate digestive enzymes and glucose movement; hence, it is significantly reduced the post-postprandial hyperglycemia.

Keywords: Seagrass, Halophila beccarii, Facilitated diffusion, Nil, Nil, Antidiabetic, Carbohydrates.

Author Biographies

Vani M, Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
Department of Appled microbiology & Biochemistry
Vasavi T, Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
Department of Appled microbiology & Biochemistry
Uma Maheswari Devi P, Department of Biochemistry and Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
Department of Appled microbiology & Biochemistry

References

1. Keerthana G, Kalaivani MK, Sumathy A. In-vitro alphaamylase inhibitory and anti-oxidant activities of ethanolic leaf extract of croton bonplandianum. Asian J Pharm Clin Res 2013;6:32-6.
2. Groop L, Forsblom C, Lehtovirta M. Characterization of the prediabetic state. Am J Hypertens 1997;10:172-80.
3. Perfetti R, Barnett PS, Mathur R, Egan JM. Novel therapeutic strategies for the treatment of Type 2 diabetes. Diabetes Metab Rev 1998;14:207-25.
4. Dineshkumar B, Mitra A, Manjunatha MA. Comparative study of alpha amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm 2010;4:115-21.
5. Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabetic potential. J Ethnopharmacol 2002;81:81-100.
6. Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol 2006;106:1-28.
7. Newmaster AF, Berg KJ, Ragupathy S, Palanisamy M, Sambandan K, Newmaster SG, et al. Local knowledge and conservation of seagrasses in the Tamil Nadu State of India. J Ethnobiol Ethnomed 2011;7:37.
8. McCue PP, Shetty K. Inhibitory effects of rosmarinic acid extracts on porcine pancreaticamylase in vitro. Asia Pac J Clin Nutr 2004;13:101-6.
9. Suthindhiran KR, Jayasri MA, Kannabiran K. α-glucosidase and α-amylase inhibitory activity of Micromonospora sp. VITSDK3(EU551238). Int J Integr Biol 2009;6:115-20.
10. Ou S, Kwok K, Li Y, Fu L. In vitro study of possible role dietary fibre in lowering postprandial serum glucose. J Agric Food Chem 2001;49:1026-9.
11. Edwards CA, Johnson IT, Read NW. Do viscous polysaccharidesslow absorption by inhibiting diffusion or convection? Eur J Clin Nutr 1988;42:307-12.
12. Ahmed F, Sairam S, Urooj A. In vitro hypoglycemic effects of selected dietary fiber sources. J Food Sci Technol 2011;s48:285-9.
13. Daksha G, Chandrashekher K, Pal G. In vitro antidiabetic activity of pentacyclic tritrprnoida and fatty acid ester from Bauhinia purpurea. Int J Pharm Technol 2013;2:2277-3436.
14. Cirillo VP. Mechanism of glucose transport across the yeast cell membrane. Bacteriology 1962;84:485-91.
15. Lee HS. Inhibitary activity of Cinnamomum cassia bark derived component against rat lens aldolase reductase. J Pharm Pharm Sci, 2002;5;226-30.
16. Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F, et al. In vitro antioxidant effect and inhibition of alpha-amylase of two varieties of amaranthus caudatus seeds. Biol Pharm Bull 2005;28: 1098-102.
17. Ceriello A, Cavarape A, Martinelli L. The post-prandial state in Type 2 diabetes and endothelial dysfunction: Effects of insulin aspart. Diabet Med 2004;21:171-5.
18. Gallagher AM, Flatt PR, Duffy G, Abdel-Wahab YH. The effects of traditional ant diabetic plants on in vitro glucose diffusion. Nutr Res 2003;23:413-24.
19. Kunyanga CN, Imungi JK, Okoth MW, Biesalski HK, Vadivel V. Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT Food Sci Technol 2012;45:269-76.
20. Mai TT, Thu NN, Tien PG, Van Chuyen N. Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol (Tokyo) 2007;53:267-76.
21. Ramkumar KM, Thayumanavan B, Palvannan T, Rajaguru P. Inhibitory effect of Gymnema montana leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content. Med Chem Res 2010;19:948-61.
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M, V., V. T, and U. M. Devi P. “EVALUATION OF IN VITRO ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF SEAGRASS HALOPHILA BECCARII”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 150-3, doi:10.22159/ajpcr.2018.v11i8.25752.
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