• Mahendra Kumar Mishra Department of Biological Sciences, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh, India. http://orcid.org/0000-0002-9380-8943
  • Ruchita Tripathi Department of Biotechnology, Govt. T. R. S. College, Rewa Madhya Pradesh, India.
  • Pandeya Kb Department of Physical Sciences, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh, India.
  • Tripathi Ip Department of Faculty of Science and Environment, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh, India.


Objective: Diabetes is complex metabolic disease having a symptom of hyperglycemia. Oxovanadium (IV) and l-amino acids are used to normalize the hyperglycemic condition. The aim of this study was to screen the α-amylase inhibitory activity of l-amino acids, their oxovanadium (IV) complexes, and electrochemical activity of oxovanadium (IV) complexes.

Methods: All the oxovanadium (IV) complexes were synthesized according to the solubility of l-amino acids; the molar ratio of metal to l-amino acid was 1:2. The synthesized oxovanadium (IV) complexes were examined for their electrochemical behavior in 0.01 M sodium perchlorate solution. Further, the oxovanadium (IV) complexes of l-amino acids and l-amino acids were screened for their α-amylase inhibitory activity using spectrophotometric assay system.

Results: The synthesized complexes were divided into four groups according to nature of amino acids. Entire complexes show simple irreversible wave for VO redox couples in −900–50 mV potential range and scan rate was 300 mV/S. All the complexes and l-amino acids were screened for their α-amylase inhibitory activity. L-Histidine and their oxovanadium (IV) complex show the minimum IC50 value, i.e. 4199.05 μM and 101.015 μM, respectively, in their respective groups.

Conclusion: The data obtained from our study, it reveals that the entire oxovanadium (IV) complexes are an irreversible wave for VO redox system and the l-histidine and its oxovanadium (IV) complex is the most potent inhibitor for the α-amylase. Further, the complexes show minimum IC50 value on comparing their respective ligands due to the interaction of Vanadyl complex to the enzyme, at the sixth vacant position of Vanadyl complex.

Keywords: Diabetes mellitus, Oxovanadium (IV) complexes, l-Amino acids, α-amylase inhibition, Cyclic voltammeter.

Author Biography

Mahendra Kumar Mishra, Department of Biological Sciences, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh, India.

Research Associate

Dept of Chemistry


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How to Cite
Mishra, M. K., R. Tripathi, P. Kb, and T. Ip. “α-AMYLASE INHIBITION AND ELECTROCHEMICAL BEHAVIOR OF SOME OXOVANADIUM (IV) COMPLEXES OF L-AMINO ACIDS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 218-24, doi:10.22159/ajpcr.2018.v11i8.25800.
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