• Melnyk Mv Department of Pharmacy, Ivan-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine.
  • Vodoslavskyi Vm Department of Pharmacy, Ivan-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine.
  • Obodianskyi Ma Department of Pharmacy, Ivan-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine.



Ruta graveolens L, Stellaria media (L) Vill, Phenolic compounds, Hydroxycinnamic acids, Flavonoids, Tannins, High-performance liquid chromatography


Objective: The results of the determination of the phenolic compounds in Ruta graveolens L. and Stellaria media (L.) Vill. herb by the method of high-performance liquid chromatography (HPLC) with ultraviolet (UV)-detection are presented in the article.

Method: The qualitative composition and quantitative content of the phenolic compounds were studied by HPLC on the Agilent 1200 chromatograph using methanolic extracts of R. graveolens L. and S. media (L.) Vill. herb. The components of hydroxycinnamic acids were determined at wavelengths of 320 nm and 330 nm; flavonoids at 255 nm and 340 nm; and tannins at 255 nm and 280 nm.

Results: On the basis of the analysis conducted by the method of HPLC in the herb of R. graveolens L., 16 substances were identified: 6 flavonoids (apigenin, rutin, quercetin, luteolin, isoquercetin, and hyperoside), 4 hydroxycinnamic acids (chlorogenic, rosmarinic, caffeic, and p-coumaric), and 6 tannins (gallic and ellagic acids, gallocatechin, epigallocatechin, epicatechin, and epicatechin gallate). In the herb of S. media (L.) Vill., chlorogenic acid, flavone aglycones: Luteolin and apigenin, and flavonol glycosides: Isoquercetin and rutin were identified.

Conclusion: According to the results of the research, it was established that the dominant components of R. graveolens L. and S. media (L.) Vill. herb are chlorogenic acid and a flavonoid component apigenin, which has anti-inflammatory activity. Thus, the obtained results point to the prospect of further research with the aim of creating of herbal substances with a certain pharmacological action.


Download data is not yet available.


Sreedevi P, Vijayalakshmi K, Venkateswari R. Phytochemical evaluation of Punica granatum L. leaf extract. Int J Curr Pharm Res 2017;9:14-8.

Marchyshyn SM, Demydiak OL, Polonets OV, Harnyk MS. Research of phenolic compounds of chrysanthemum garden perennial (Chrysanthemum hortorum Bailey). Med Clin Chem 2016;18:48-54.

Janaki A, Kaleena PK, Elumalai D, Hemalatha P, Babu M, Velu K, et al. Phytochemical screening, antioxidant and antibacterial activites of Millingtonia hortensis (L). Int J Curr Pharm Res 2017;9:162-7.

YeS T. Phytotherapy. Kyiv: Oriiany; 2000.

Hrodzynskyi AM. Medicinal plants: Encyclopedic guide. Kyiv: Ukrainian Encyclopedia MP Bazhana; 1990.

Asgarpanah J, Khoshkam R. Phytochemistry and pharmacological properties of Ruta graveolens L. J Med Plants Res 2012;6:3942-9.

YaP D, Pliuta PH, Protopopova VV. Ecoflora of Ukraine. Kyiv: Fitosotsiotsentr; 2000.

Fedoronchuk MM. Family of Caryophyllaceae Jess. In the flora of Ukraine: Systematics, Geography, History of Development: Author. Dis Doc. of Biology Sciences. Kiev: 03.00.05 Botanyâ€. Institute of Botany M.G. Cold NAS of Ukraine; 2006.

Arora D, Sharma A. Pharmacognostic and phytochemical studies of Stellaria media Linn. J Pharm Sci Res 2012;4:1819-22.

Bukola OO, Bernard SA. Phytochemistry and in vitro antioxidant activities of Stellaria media, Cajanus cajanand, Tetracera potatoria methanolic extracts. J Med Plants Res 2011;5:6622-7.

Kitanov GM. Phenolic acids and flavonoids from Stellaria media (L.) Vill Caryophyllaceae. Die Pharmazie 1992;47:470-1.

Koshovyi OM, YeO P, Kovaliova AM. Research of phenolic compounds of leaves of medicinal sage. Pharm J 2010;1:17-9.

Popov DM, Naumov AV. Investigation of a starved medium by means of an HPLC method. Pharmacy 2012;2:21-3.

Musienko SG, Kyslychenko VS. The quantitative content of the main groups of biologically active substances in the bay Laurel raw material. News Pharm 2014;4:22-4.

Hu YM, Ye WC, Li Q, Tian HY, Wang H, Du HY. C-glycosylflavones from Stellaria media. J Nat Med 2006;4:420-4.

Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005;26:343-56.

Sreedevi P, Vijayalakshmi K. Determination of antioxidant capacity and gallic acid content in ethanolic extract of Punica granatum L. Leaf. Asian J Pharm Clin Res 2018;11:319-23.

Shuchi DM, Sarvesh P. Phytochemical analysis, liquid chromatography, and mass spectroscopy and in vitro anticancer activity of Annona squamosa seeds Linn. Asian J Pharm Clin Res 2018;11:101-3.



How to Cite

Mv, M., V. Vm, and O. Ma. “RESEARCH OF PHENOLIC COMPOUNDS OF RUTA GRAVEOLENS L. AND STELLARIA MEDIA (L.) VILL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 9, Sept. 2018, pp. 152-6, doi:10.22159/ajpcr.2018.v11i9.25920.



Original Article(s)