CORRELATION OF ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF AMLA AND GINGER

  • Safeena Kulsum Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, #258/A, 8th Floor, A-Block, Mazumdar Shaw Medical Centre, Narayana Health, Bommasandra Industrial Area, Anekal Taluk, Bengaluru - 560 099, Karnataka, India.
  • Amritha Suresh Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, #258/A, 8th Floor, A-Block, Mazumdar Shaw Medical Centre, Narayana Health, Bommasandra Industrial Area, Anekal Taluk, Bengaluru - 560 099, Karnataka, India.
  • Alka Mehta Department of Medical Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.

Abstract

Objective: Our study focused on evaluating the anticancer property of ascorbic acid and aqueous extract of amla and ginger.

Methods: Antioxidant capacity of ascorbic acid, aqueous extract of amla, and ginger was obtained by 2,2-diphenyl-1-picrylhydrazyl method and total antioxidant capacity (TAC). Vero cell line, PA-1, Cal-27, Cal-27 CisR, and DysMSCTR16 cell lines were treated with antioxidants to evaluate its antiproliferative property using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Colony and spheroid formation assays were also carried out in the presence of extracts to assess its role in anticancer stem cell activity.

Results: Two volumes of amla 5 μl (~0.016 g) and 25 μl (~0.08 g) and ginger 5 μl (~0.02 g) and 25 μl (~0.1 g) showed TAC activity equivalent to 0.25 mM and 2 mM ascorbic acid, respectively. Amla and ascorbic acid showed significant antiproliferative property in normal (Vero) p=0.05, cancer (PA-1, Cal-27) p=0.005, and resistant (Cal-27 CisR) p=0.05 cell lines and ginger extract in Vero and Cal-27 cell lines (p=0.05). In PA-1 and Cal-27 CisR cell line, ginger extract showed proliferative activity (p=0.005). Antioxidants showed no antiproliferative activity in DysMSCTR16 cells. Amla extract and ascorbic acid showed significant inhibitory effect on spheroid (p=0.005) and colony formation capacity (p=0.0005) among dysplastic, cancer, and resistant cell lines. Ginger showed inhibitory effect (p=0.05) only in colony formation capacity.

Conclusion: Overall, we found a strong correlation between antioxidant and antiproliferative activity of ascorbic acid, amla, and ginger. Amla and ascorbic acid proved to be effective in controlling cell proliferation and self-renewal properties of cancer cells. However, ginger was found to have selective and less antiproliferative effect in comparison to amla.

Keywords: Amla, Ginger aqueous extract, Cancer progression, Cancer stem cells, Antioxidant-induced cell death.

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Kulsum, S., A. Suresh, and A. Mehta. “CORRELATION OF ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF AMLA AND GINGER”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 263-9, doi:10.22159/ajpcr.2018.v11i8.26073.
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