• Safeena Kulsum Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, #258/A, 8th Floor, A-Block, Mazumdar Shaw Medical Centre, Narayana Health, Bommasandra Industrial Area, Anekal Taluk, Bengaluru - 560 099, Karnataka, India.
  • Amritha Suresh Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, #258/A, 8th Floor, A-Block, Mazumdar Shaw Medical Centre, Narayana Health, Bommasandra Industrial Area, Anekal Taluk, Bengaluru - 560 099, Karnataka, India.
  • Alka Mehta Department of Medical Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.


Objective: Our study focused on evaluating the anticancer property of ascorbic acid and aqueous extract of amla and ginger.

Methods: Antioxidant capacity of ascorbic acid, aqueous extract of amla, and ginger was obtained by 2,2-diphenyl-1-picrylhydrazyl method and total antioxidant capacity (TAC). Vero cell line, PA-1, Cal-27, Cal-27 CisR, and DysMSCTR16 cell lines were treated with antioxidants to evaluate its antiproliferative property using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Colony and spheroid formation assays were also carried out in the presence of extracts to assess its role in anticancer stem cell activity.

Results: Two volumes of amla 5 μl (~0.016 g) and 25 μl (~0.08 g) and ginger 5 μl (~0.02 g) and 25 μl (~0.1 g) showed TAC activity equivalent to 0.25 mM and 2 mM ascorbic acid, respectively. Amla and ascorbic acid showed significant antiproliferative property in normal (Vero) p=0.05, cancer (PA-1, Cal-27) p=0.005, and resistant (Cal-27 CisR) p=0.05 cell lines and ginger extract in Vero and Cal-27 cell lines (p=0.05). In PA-1 and Cal-27 CisR cell line, ginger extract showed proliferative activity (p=0.005). Antioxidants showed no antiproliferative activity in DysMSCTR16 cells. Amla extract and ascorbic acid showed significant inhibitory effect on spheroid (p=0.005) and colony formation capacity (p=0.0005) among dysplastic, cancer, and resistant cell lines. Ginger showed inhibitory effect (p=0.05) only in colony formation capacity.

Conclusion: Overall, we found a strong correlation between antioxidant and antiproliferative activity of ascorbic acid, amla, and ginger. Amla and ascorbic acid proved to be effective in controlling cell proliferation and self-renewal properties of cancer cells. However, ginger was found to have selective and less antiproliferative effect in comparison to amla.

Keywords: Amla, Ginger aqueous extract, Cancer progression, Cancer stem cells, Antioxidant-induced cell death.


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How to Cite
Kulsum, S., A. Suresh, and A. Mehta. “CORRELATION OF ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF AMLA AND GINGER”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 263-9, doi:10.22159/ajpcr.2018.v11i8.26073.
Original Article(s)