CURCUMINOIDS ANALYSIS IN CURCUMA MANGGA RHIZOMES
Keywords:Curcuma mangga, Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin, Ultra-performance liquid chromatography
Objective: The current study was conducted to analyze the curcuminoid content in ethanol extract of Curcuma mangga.
Methods: The constituent of ethanol extract of C. mangga was determined using ultra-performance liquid chromatography (UPLC). The diluted solutions of the extracts and the reference standards were analyzed separately by the reversed-phase UPLC method with mobile phase of acetonitrile: water (40:60) isocratically eluted for 30 min.
Results: The chromatograms of the ethanol extract of C. mangga showed the presence of curcumin, bisdemethoxycurcumin, and demethoxycurcumin (DMC). Of all the curcuminoids content, DMC was the major component, corresponding to retention time at 9.96 min as compared to curcuminoid standard at 9.69 min.
Conclusion: The results indicate that ethanol extract of C. mangga rhizomes contains DMC at the highest amount as compared to other curcuminoid compounds.
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