IN VITRO ANTI-ARTHRITIC ACTIVITY OF CISSUS QUADRANGULARIS STEM EXTRACT

  • Vaijayanthimala P Department of Pharmaceutical Chemistry, SSM College of Pharmacy, Erode - 638 312, Tamil Nadu, India.
  • Sakthipriya M Department of Pharmaceutical Chemistry, SSM College of Pharmacy, Erode - 638 312, Tamil Nadu, India.
  • Sangameswaran B Department of Pharmaceutical Chemistry, SSM College of Pharmacy, Erode - 638 312, Tamil Nadu, India.

Abstract

Objective: The present investigation deals with the study of in vitro anti-arthritic activity by inhibition of protein denaturation method by bovine serum albumin method and egg albumin method. Cissus quadrangularis Linn plant is a perennial tendril climber with quadrangular stem. It is used in the treatment of gout, syphilis, stomach ache, regularized the menstrual cycle, antimicrobial activity, and piles in Ayurvedic medicine, and traditionally used for the bone fracture.

Method: The air-dried powder of C. quadrangularis Linn (stem parts) was extracted using a Soxhlet apparatus with methanol C. quadrangularis (MECQ) and aqueous C. quadrangularis water (AECQ) as solvent. The extracts were concentrated under reduced pressure. The activities were carried out using the following concentration (100, 200, 300, 400, and 500 μg/ml) and compared with diclofenac as standard drug. It has significant in vitro anti-arthritic in both the methods.

Result: The extract of C. quadrangularis possessed significant anti-arthritic property in MECQ than compared to AECQ.

Conclusion: Activity may be due to the presence of the chemical profile such as phenolic acid, flavonoid (leuteotin), and β-sitosterol. The results of the study have suggested in the use of C. quadrangularis Linn as a potent anti-arthritic in several applications.

Keywords: Cissus quadrangularis Linn, Anti-arthritic, Bovine serum albumin, Methanol, Diclofenac.

Author Biography

Vaijayanthimala P, Department of Pharmaceutical Chemistry, SSM College of Pharmacy, Erode - 638 312, Tamil Nadu, India.

professor

Department of pharmaceutical chemistry

References

1. Vaijayanthimala P, Sureshkumar S, Sangameswaran B. Gastroprotective activity of Cucumis sativus. World J Pharm Pharm Sci 2015;4:457-64.
2. Srikanth N, Elumalai A, Eswaraiah MC, Veldi N. An updated review on anti-arthritic medicinal plant. Int J Pharm Sci Rev Res 2012;2:11-5.
3. Montecucco F, Mach F. Common inflammatory mediators orchestrate pathophysiological processes in rheumatoid arthritis and atherosclerosis. Rheumatology 2009;48:11-2.
4. Wang M, Li K, Nie Y, Wei Y, Li X. A network study of Chinese medicine xuesaitong injection to elucidate a complex mode of action with multicompound, multitarget, and multipathway. Evid Based Complement Alternat Med 2012;12:1-10.
5. Pavithra TK, Smitha KP, Kulashekar KS, Kumar BS. Evaluation of in vitro anti-arthritic activity of Vitex negundo against the denaturation of protein. Int J Curr Microbiol Appl Sci 2015;4:87-90.
6. Jagtap VA, Agasimundim YS, Jayachandran E, Sathes BS. In vitro anti-inflammatory activity of 2- amino-3-(substituted benzylidine carbohydrazide)-4,5,6,7- tetrahydrobenzo-thiophenes. J Pharm Res 2011;4:378-9.
7. Sumanya H, Lavanya R, Reddy C. Evaluation of in vitro anti-oxidant and anti-arthritic activity of methanolic extract of marine green algae Caulerpa racemosa. Int J Pharm Pharm Sci 2015;7:340-3.
8. Ghouse MS. A pharmacognostical review on Cissus quadrangularis Linn. Int J Res Pharm Biosci 2015;2:28-35.
9. Kumari CS, Yasmin1 N, Raffiq M, Hussain N, Babuselvam M. In vitro anti-inflammatory and anti-arthritic property of Rhizopora mucronata leaves. Int J Pharm Sci Res 2016;6:482-5.
10. Lavanya R, Maheshwari SU, Harish G, Raj JB, Kamali S, Hemamalani D. Investigation of in vitro anti-inflammatory, anti-platelet and antiarthritic activities in the leaves of Anisomeles malabarica Linn. Res J Pharm Biol Chem Sci 2010;1:746-52.
11. Srividya S, Sridevi G. Anti-arthritic and anti-inflammatory activity of ethanolic leaf extract of Ormocarpum sennoides. Int J Pharm Pharm Sci 2016;8:117-21.
12. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.
13. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of anti-inflammatory effect of ashwagandha: A preliminary study in vitro. Pharmacogn J 2012;4:47-9.
14. Sangeetha M, Kousalya K, Lavanya R, Sowmya C, Chamundeeswari D, Maheswara UC. In vitro antiinflammatory and anti-arthritic activity of leaves of Clerodendron inerme. Res J Pharm Biol Chem Sci 2011;2:822 7.
Statistics
125 Views | 149 Downloads
Citatons
How to Cite
P, V., S. M, and S. B. “IN VITRO ANTI-ARTHRITIC ACTIVITY OF CISSUS QUADRANGULARIS STEM EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 250-2, doi:10.22159/ajpcr.2019.v12i1.27353.
Section
Original Article(s)