STUDY OF ANTIOBESITY ACTIVITY OF POLYHERBAL FORMULATION IN CORRELATION WITH ANTIDIABETIC ACTIVITY
Objective: The objective of this research was to develop a polyherbal formulation using four different herbs using obese diabetic rat's model.
Methods: Rats received high-fat diet and alloxan was injected intraperitoneal to rats for induction of diabetes. In the preventive experiment, diabetic rats received Momordica charantia Linn. (200 and 400 mg/kg/day p.o), Eugenia jambolana Linn. (200 and 400 mg/kg/day p.o), Ziziphus mauritiana (200 and 400 mg/kg/day p.o), Acacia catechu (AC) (200 and 400 mg/kg/day p.o), and aqueous extract of all extracts (100 and 200 mg/kg/day p.o). Diabetic rats were also treated with glibenclamide (5 mg/kg p.o.) and orlistat (60 mg/kg/day p.o.) as reference standards.
Results: The results showed that the extract of M. charantia Linn., E. jambolana Linn., Z. mauritiana, and AC significantly (p<0.05) inhibited body weight gain, blood glucose, triglyceride, total cholesterol, low-density lipoprotein (LDL), very LDL, high-density lipoprotein cholesterol, serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, and fasting blood glucose in a dose-dependent manner. Extracts treated rats at doses of 200 and 400 mg/kg improved dyslipidemia in high-fat diet (HFD)-induced obese rats by enhancing their lipid metabolism when compared to the HFD control.
Conclusion: The results obtained in this research work clearly showed that taken together the extract of M. charantia Linn., E. jambolana Linn., Z. mauritiana Lam. AC Willd., and aqueous extract of all extracts has potential as a preventive agent for type 2 diabetes mellitus (and possibly obesity) and deserves clinical trial in the near future.
2. Power ML, Schulkin J. Sex differences in fat storage, fat metabolism and the health risks from obesity: Possible evolutionary origins. Br J Nutr 2008;99:931-40.
3. Afolayan HJ, Mbaebie BO. Ethnobotanical study of medicinal plants in nkonkobe municipality in South Africa. Pharmacogn J 2010;2:368-74.
4. Hotamisligil GS, Erbay E. Nutrient sensing and inflammation in metabolic diseases. Nat. Rev Immunol 2008;8:923-34.
5. Balakumar P, Chakkarwar VA, Singh M. Ameliorative effect of combination of benfotiamine and fenofibrate in diabetes-induced vascular endothelial dysfunction and nephropathy in the rat. Mol Cell Biochem 2009;320:149-62.
6. Luis-RodrÃguez D, MartÃnez-Castelao A, Gorriz JL, De-Alvaro F, Navarro-Gonzalez JF. Pathophysiological role and therapeutic implications of inflammation in diabetic nephropathy. World J Diabetes 2012;15:7-18.
7. Deshmukh CD, Jain A. Antidiabetic and antihyperliperdermic effect of methanolic extract of Citrullus lanatus seeds in rats. Int J Pharm Pharm Sci 2009;7:232-6.
8. Goedecke JH, Dave JA, Faulenbach MV. Insulin response in relation to insulin sensitivity: An appropriate beta-cell response in black South African women. Diabetes Care 2009;32:860-5.
9. Shrivastava SP, Mishra A, Laxmi V, Tamrakar AK, Shrivastava MN, Shrivastava AK. Antidiabetic and antidyslipidermic activity of ethyl acetate fraction of Xylocarpus granatum and Xylocarpus molluccensis on high fructose high fat and high sucrose high fat fed-low dosed streptozotocin treated diabetic rats. Int J Pharm Pharm Sci 2015;7:537- 43.
10. Habicht SD, Kind V, Rudloff S, Borsch C, Mueller AS, Pallauf J, et al. Quantification of antidiabetic extracts and compounds in bitter gourd varieties. Food Chem 2011;126:172-6.
11. Subratty AH, Gurib-Fakim A, Mahomoodally F. Bitter melon: An exotic vegetable with medicinal values. Nutr Food Sci 2005;35:143-7.
12. Sagrawat H, Mann AS, Kharya MD. Pharmacological potential of Eugenia jambolana: A review. Pharmacogon Mag 2006;2:96-105.
13. Dahiru D, William ET, Nadro MS. Protective effect of Ziziphus mauritiana leaf extract on carbon tetrachloride-induced liver injury. African J Biotechnol 2005;4:1177-9.
14. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. New Delhi: Springer Science & Business Media; 2008.
15. Kirtikar KR, Basu BD. India Medicinal Plants. Vol. 2. New Delhi, India: Periodical Experts; 1975.
16. Lee A, Morley JE. Metformin decreases food consumption and induces weight loss in subjects with obesity with Type II noninsulin dependent diabetes. Obes Res 1998;6:47-53.
17. Schermer S. The Blood Morphology of Laboratory Animals. Los Altos, California: Longmans, Green and Co. Ltd.; 1967. p. 350.
18. Yuen VG, McNeill JH. Comparison of the glucose oxidase method for glucose determination by manual assay and automated analyzer. J Pharmacol Toxicol Methods 2000;44:543-6.
19. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56-63.
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