DEVELOPMENT OF POLYHERBAL WITH ANTIOXIDANT ACTIVITY

  • Jain Deepak Centre for Research and Development, Pacific Academy of Higher Education and Research University, Udaipur - 313 024, Rajasthan, India.
  • Jain Anurekha Dean, Jayoti Vidyapeeth Women’s University, Jaipur – 303 122, Rajasthan, India.

Abstract

Objective: The objective of this research was to develop a polyherbal formulation (PHF) using four different herbs and to evaluate their phytochemicals, physical constants, and determination of their antioxidant activity by DPPH method.

Methods: The PHF authenticated herbs were characterized by studying its morphological and pharmacognostic characters. Phytochemical screening showed the presence of alkaloids, glycosides, carbohydrates, amino acid, tannin, steroids, and flavonoids in the combination extract. Physical parameters such as loss on drying (LOD), pH, ash values, LOD, and extractive value have been studied. The antioxidant activity of the combination of extract (100 mg each) was determined using DPPH free radical scavenging method.

Results: The results showed that the combination extract has best antioxidant effect at a dose of 400 μg/ml when it was compared with ascorbic acid as the reference standard.

Conclusion: The results obtained in this research work clearly showed that the combination extract has best antioxidant effect at a dose of 400 μg/ ml when it was compared with ascorbic acid as the reference standard.

 

Keywords: Polyherbal formulation (PHF), Phytochemical, Antioxidant activity, Ascorbic acid.

References

1. Khopde SM, Darshini P, Mohan H, Gawandi VB. Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract. Curro Sci 2001;81:185-190.
2. Irda F, Evelyne N, Komar RW. In vitro antioxidant activities, total flavonoid, phenolic and carotenoid content from various extracts of four species asteraceae herb. Int J Pharm Pharm Sci (IJPPS) 2015;7:192-7.
3. Well H, Gutteridge JM. Free Radicals in Biology and Medicine OS’ed. Oxford: Clarendon Press; 1985.
4. Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann N Y Acad Sci 2000;899:136- 47.
5. Zerargui F, Baghiani A, Khennouf S, Arrar L. Antioxidant activity assessment of Tamus communis L. Roots. Int J Pharm Pharm Sci (IJPPS) 2016;8:64-71.
6. Jarald EE, Joshi SB, Jain DC. Biochemical study on the hypoglycemic effect of extracts and fractions of Acacia catechu Willd. in alloxan induced diabetic rats. Int J Diabetes Metab 2009;17:62-9.
7. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 3rd ed. Pune: Nirali Prakashan; 1995. p. 218-9.
8. Khandewal KR. Practical Pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008. p. 24-7.
9. Alam MN, Bristi NJ. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143-52.
Statistics
148 Views | 251 Downloads
Citatons
How to Cite
Deepak, J., and J. Anurekha. “DEVELOPMENT OF POLYHERBAL WITH ANTIOXIDANT ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 483-5, doi:10.22159/ajpcr.2018.v11i8.27791.
Section
Original Article(s)