EFFECT OF LEVETIRACETAM ON HEALING OF EXCISION AND BURN WOUND MODELS IN WISTAR RATS
Objective: Levetiracetam (LEV) at lower doses (50 and 100 μg/ml) has demonstrated antioxidant effect as per literature evidence. Hence, the aim of current study was to evaluate the action of LEV on wound healing with regard to its antioxidant properties.
Methods: A total of 30 adult rats of both the sexes weighing 150–250 g were randomly divided into five groups of six rats each. Excision and burn models were used. The five groups are as follows: Group 1: Normal saline, Group 2: Povidone-iodine, Group 3: Silver sulfadiazine, Group 4: LEV 50 mg/kg, and Group 5: LEV 100 mg/kg. Group 2 served as a standard control for excision wound model and Group 3 as a standard for burn wound model. LEV was given orally and all other drugs were given topically. The rate of wound contracture and period of epithelization were calculated from the initial wound size for all rats.
Results: In both the models, the rats treated with standard drug (povidone-iodine/silver sulfadiazine) and LEV showed faster rates of wound contraction, period of epithelization, and better antioxidant effect as compared to the control and the difference seen was statistically significant (p<0.001). However, the rats treated with povidone-iodine/silver sulfadiazine showed faster rates of wound contraction and period of epithelization as compared to the rats treated with LEV and the difference was statistically significant (p<0.05).
Conclusion: The results of the study show that oral application of LEV enhanced the wound strength and accelerated healing of incision and burn wounds in rats. Further clinical trials can be done to prove the effect of LEV as an agent for wound healing.
2. Karodi R, Jadhav M, Rub R, Bafna A. Evaluation of the wound healing activity of crude extract of Rubia cordifolia L. (Indian madder) in mice. Int J Appl Res Nat Prod 2009;2:12-8.
3. Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res 2010;89:219-29.
4. Vinothapooshan G, Sundar K. Wound healing effect of various extracts of Adathoda vasica. Int J Pharm Bio Sci 2010;1:530-6.
5. Patil N, Vaishnav R, Thanusubramanian H, Holla SN, Manohar HD, Bairy KL. Formulation and evaluation of the hydroalcoholic extract of Caesalpinia pulcherrima (Stem bark) on wound healing model in Wistar rats. Int J Adv Res 2015;3:648-54.
6. Gibbs JE, Walker MC, Cock HR. Levetiracetam: Antiepileptic properties and protective effects on mitochondrial dysfunction in experimental status epilepticus. Epilepsia 2006;47:469-78.
7. Lyseng-Williamson KA. Levetiracetam: A review of its use in epilepsy. Drugs 2011;71:489-514.
8. Oliveira AA, Almeida JP, Freitas RM, Nascimento VS, Aguiar LM, Júnior HV, et al. Effects of levetiracetam in lipid peroxidation level, nitrite-nitrate formation and antioxidant enzymatic activity in mice brain after pilocarpine-induced seizures. Cell Mol Neurobiol 2007;27:395-406.
9. Ueda Y, Doi T, Takaki M, Nagatomo K, Nakajima A, Willmore LJ, et al. Levetiracetam enhances endogenous antioxidant in the hippocampus of rats: In vivo evaluation by brain microdialysis combined with ESR spectroscopy. Brain Res 2009;1266:1-7.
10. Kurahashi T, Fujii J. Roles of antioxidative enzymes in wound in wound healing. J Dev Biol 2015;3:57-70.
11. Oliveira AD, Linhares MI, Filho AJ, Rios ER, Lima CN, Venancio ET, et al. Antioxidant properties of antiepileptic drugs levetiracetam and clonazepam in mice brain after in vitro-induced oxidative stress. Afr J Pharm Pharmacol 2016;10:278-88.
12. Morton JJ, Malone MH. Evaluation of vulneray activity by an open wound procedure in rats. Arch Int Pharmacodyn Ther 1972;196:117-26.
13. Ansell DM, Holden KA, Hardman MJ. Animal models of wound repair: Are they cutting it? Exp Dermatol 2012;21:581-5.
14. Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health: A review. Crit Rev Food Sci Nutr 1998;38:421-64.
15. Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: A comprehensive review. Adv Wound Care (New Rochelle) 2014;3:445-64.
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