ANALGESIC PROPERTY OF ANGIOTENSIN AT1 RECEPTOR ANTAGONIST: CANDESARTAN IN RATS AND MICE

Authors

  • Asha D Jadhav Junior Resident
  • Rakesh R Jadhav
  • Sudhir L Padwal
  • Vinod S Deshmukh
  • Harshal N Pise
  • Swapnil S Jadhav

Abstract

 

 Objective: The objective was to evaluate analgesic activity of candesartan in graded dose in tail flick method in rats and acetic acid-induced writhing in mice.

Methods: Wistar Albino rats of either sex weighing 200-250 g or Swiss Albino mice of either sex weighing 20-25 g. Analgesic activity of candesartan (5 mg/kg, 10 mg/kg, 15 mg/kg.) was evaluated in graded dose and compared with tramadol (10 mg/kg) and aspirin (100 mg/kg) using tail flick response method and acetic acid-induced writhing of analgesia. Study was conducted after approval from the Institutional Animal Ethics Committee, which is an approved body by Committee for the Purpose of Control and Supervision of Experiments on Animals letter no. 78 dated October 18, 2012.

Results: In the present study, oral administration of candesartan showed analgesic activity at high dose compared to the control and less analgesic activity as compared to the standard in analgesic methods. In tail-flick method, after 30-90 minutes of drug administration, tail flick latency of candesartan (15 mg/kg) was significant (p<0.05) compared with control but less than that of tramadol and aspirin. In acetic acid induced writhing method, the analgesic activity of candesartan was significant only at high dose (15 mg/kg) compared to the control.

Conclusion: Candesartan possesses analgesic activity only at high dose. However, further studies need to be carried out to see underlying mechanism candesartan in analgesia and to know the extent of analgesia.

Keywords: Acetic acid induced writhing method, Angiotensin II receptor blockers, Candesartan, Tail flick method

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References

Grover SA, Coupal L, Zowall H. Treating osteoarthritis with cyclooxygenase-2-specific inhibitors: What are the benefits of avoiding blood pressure destabilization? Hypertension 2005;45(1):92-7.

Welch WJ. Angiotensin II-dependent superoxide: Effects on hypertension and vascular dysfunction. Hypertension 2008;52(1):51-6.

D’amour FE, Smith DL. A method for determining loss of pain sensation. Indian J Exp Pharmacol Ther 1941;72(1):74-9.

Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Fed Proc 1959;18:412-8.

Rüster C, Wolf G. Renin-angiotensin-aldosterone system and progression of renal disease. J Am Soc Nephrol 2006;17:2985-91.

Rajagopalan S, Kurz S, Münzel T, Tarpey M, Freeman BA, Griendling KK, et al. Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. Contribution to alterations of vasomotor tone. J Clin Invest 1996;97(8):1916-23.

Dai Q, Xu M, Yao M, Sun B. Angiotensin AT1 receptor antagonists exert anti-inflammatory effects in spontaneously hypertensive rats. Br J Pharmacol 2007;152(7):1042-8.

Bregonzio C, Armando I, Ando H, Jezova M, Baiardi G, Saavedra JM. Anti-inflammatory effects of angiotensin II AT1 receptor antagonism prevent stress-induced gastric injury. Am J Physiol Gastrointest Liver Physiol 2003;285(2):G414-23.

Abdel Salam OM, El-Shenawy S, Nofal SM. Effect of Ramipril, valsartan and candesartan on thermal and visceral pain in mice. J Pharmacol Toxicol 2007;2(6):533-41.

Marshall TG, Lee RE, Marshall FE. Common angiotensin receptor blockers may directly modulate the immune system via VDR, PPAR and CCR2b. Theor Biol Med Model 2006;3:1.

Takai S, Song K, Tanaka T, Okunishi H, Miyazaki M. Antinociceptive effects of angiotensin-converting enzyme inhibitors and an angiotensin II receptor antagonist in mice. Life Sci 1996;59(21):PL331-6.

Published

01-11-2014

How to Cite

Jadhav, A. D., R. . R. Jadhav, S. . L. Padwal, V. S. Deshmukh, H. N. Pise, and S. . S. Jadhav. “ANALGESIC PROPERTY OF ANGIOTENSIN AT1 RECEPTOR ANTAGONIST: CANDESARTAN IN RATS AND MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 5, Nov. 2014, pp. 170-2, https://innovareacademics.in/journals/index.php/ajpcr/article/view/2806.

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