EVALUATION OF HYPOLIPIDEMIC ACTIVITY OF FRUIT RIND EXTRACTS OF GARCINIA GUMMIGUTTA IN DIET-INDUCED HYPERLIPIDEMIC RATS

  • BARATHANE DATCHANAMURTY Department of Pharmacology, Vinayaka Missions Medical College, Vinayaka Missions Research Foundation (Deemed to be University), Karaikal, Pondicherry, India.
  • MANIMEKALAI K Department of Pharmacology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth University, Pondicherry, India.
  • KARTIK J SALWE Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth University, Pondicherry, India

Abstract

Objective: The objective of the study was to evaluate the hypolipidemic activity of fruit rind extracts of Garcinia gummi-gutta in diet-induced hyperlipidemic rats.


Methods: Hydroalcoholic extract of fruit rinds of G. gummi-gutta was prepared using Soxhlet apparatus. 30 Wistar albino rats were divided into five groups (n=6). Except for Group 1, all the other groups were treated with hyperlipidemic diet (HLD) for 4 weeks. After induction, the Groups 3 and 4 were treated with the extracts at 100 mg/kg and 200 mg/kg doses, respectively, whereas Group 5 received the standard drug atorvastatin at 10 mg/kg for the next 4 weeks. Lipid profile was estimated at the end of each week. At the end of the study, rats were sacrificed and the liver and heart were analyzed for histopathological changes. Data expressed as mean±standard error of the mean. Statistical analysis was done using one-way ANOVA followed by post hoc Tukey test. p<0.05 considered statistically significant.


Results: The serum levels of total cholesterol (TC), triglycerides (TG), very low-density lipoprotein (VLDL), and LDL were significantly increased and the levels of high-density lipoprotein (HDL) were significantly decreased when compared to normal control. The groups which received G. gummi-gutta rind extract at 100 mg/kg and 200 mg/kg showed a significant decrease in the mean cholesterol, TG, LDL, VLDL levels, and an increase in HDL levels when compared to the HLD group. The group which received the extract at higher dose (REGG200) was found better than the lower dose (REGG 100). REGG 200 showed significant improvement in increasing the HDL levels than all the test groups. The standard drug atorvastatin was found better than REGG100 but there was no significant difference between high doses of the extract and atorvastatin.


Conclusion: The fruit rinds extract of G. gummi-gutta was able to decrease the elevated serum lipids (TC, TG, LDL, and VLDL) and consequently increase the HDL levels in a dose-dependent manner.

Keywords: Garcinia gummi-gutta, Hypolipidemic, Atorvastatin, Lipid profile.

Author Biographies

BARATHANE DATCHANAMURTY, Department of Pharmacology, Vinayaka Missions Medical College, Vinayaka Missions Research Foundation (Deemed to be University), Karaikal, Pondicherry, India.

Assistant professor - Pharmacology

MANIMEKALAI K, Department of Pharmacology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth University, Pondicherry, India.

Professor - Pharmacology

KARTIK J SALWE, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth University, Pondicherry, India

Associate Professor - Pharmacology

References

1. Abegunde DO, Mathers CD, Adam T, Ortegon M, Strong K. The burden and costs of chronic diseases in low-income and middle-income countries. Lancet 2007;370:1929-38.
2. Sample Registration System. Million Death Study: Preliminary Report on Causes of Death in India 2001-2003. New Delhi: Registrar General of India; 2007.
3. Indrayan A. Forecasting vascular disease cases and associated mortality in India. Reports of the National Commission on Macroeconomics and Health. India: Ministry of Health and Family Welfare; 2005.
4. Kondo A, Li J, Manabe M, Saito K, Kanno T, Maekawa M, et al. Relationship between high-density lipoprotein-cholesterol and malondialdehyde-modified low-density lipoprotein concentrations. J Atheroscler Thromb 2003;10:72-8.
5. Achuthan CR, Padikkala J. Hypolipidemic effect of Alpinia galanga (Rasna) and Kaempferia galanga (Kachoori). Ind J Clin Biochem 1997;12:55-8.
6. Stohs SJ, Preuss HG, Ohia S, Kaats G, Keen C, Williams L, et al. Safety and efficacy of hydroxycitric acid derived from Garcinia cambogia – A literature review. Herb Gram 2010;85:58-63.
7. Tharachand Selvaraj I, Avadhani M. Medicinal properties of Malabar tamarind [Garcinia cambogia (Gaertn.) Desr.]. Int J Pharm Sci Rev Res 2013;19:101-7.
8. Mahanta M, Mukherjee AK. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts. J Ethnopharmacol 2001;75:55-60.
9. Blank B, Pfeiffer FR, Greenberg CM, Kerwin JF. Thyromimetics. II. The synthesis and hypocholesteremic activity of some beta-diethylaminoethyl esters of iodinated thyroalkanoic acids. J Med Chem 1963;6:560-3.
10. Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol 2001;21:15-23.
11. Niehaus WG Jr., Samuelsson B. Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968;6:126-30.
12. Stocks J, Dormandy TL. The autoxidation of human red cell lipids induced by hydrogen peroxide. Br J Haematol 1971;20:95-111.
13. Carleton HM, Drury RA, Wallington EA. General staining procedures. In: Carleton’s Histological Technique. 5th ed. New York: Oxford University Press; 1980. p. 267.
14. Gogoi A, Gogoi N, Neog B. Dubious anti-obesity agent HCA from garcinia: A systematic review. Int J Pharm Pharm Sci 2015;7:1-8.
15. Sugden MC, Watts DI, Marshall CE, McCormack JG. Brown-adipose-tissue lipogenesis in starvation: Effects of insulin and (-) hydroxycitrate. Biosci Rep 1982;2:289-97.
16. Altiner A, Ates A, EsenGursel F, Bilal T. Effect of the antiobesity agent Garcinia cambogia extract on serum lipoprotein (A), apolipoproteins A1 and B, and total cholesterol levels in female rats fed atherogenic diet. J Anim Plant Sci 2012;22:872-7.
17. Offermanns S. Free fatty acid (FFA) and hydroxy carboxylic acid (HCA) receptors. Annu Rev Pharmacol Toxicol 2014;54:407-34.
18. Yokozawa T, Cho EJ, Sasaki S, Satoh A, Okamoto T, Sei Y, et al. The protective role of Chinese prescription Kangen-Karyu extract on diet-induced hypercholesterolemia in rats. Biol Pharm Bull 2006;29:760-5.
19. Nofer JR, Kehrel B, Fobker M, Levkau B, Assmann G, von Eckardstein A, et al. HDL and arteriosclerosis: Beyond reverse cholesterol transport. Atherosclerosis 2002;161:1-6.
20. Libby P, Ridker PM, Maseri A. Inflammation and atherosclerosis. Circulation 2002;105:1135-43.
21. Ramos RR, Saenz JF, Aguilar MC. Control of obesity with Garcinia cambogia extract. Invest Med Int 1996;22:97-100.
22. Mahendran P, Devi CS. Effect of Garcinia cambogia extract on lipids and lipoprotein composition in dexamethasone administered rats. Indian J Physiol Pharmacol 2001;45:345-50.
23. Preuss HG, Bagchi D, Bagchi M, Rao CV, Dey DK, Satyanarayana S. Effects of a natural extract of (–)-hydroxycitric acid (HCA-SX) and a combination of HCA-SX plus niacin-bound chromium and Gymnema sylvestre extract on weight loss. Diabetes Obes Metab 2004;6:171-80.
24. Koshy AS, Anila L, Vijayalakshmi NR. Flavonoids from Garcinia cambogia lower lipid levels in hypercholesterolemic rats. Food Chem 2001;72:289-94.
25. Leonhardt M, Münch S, Westerterp-Plantenga M, Langhans W. Effects of hydroxycitrate, conjugated linoleic acid, and guar gum on food intake, body weight regain, and metabolism after body weight loss in male rats. Nutr Res 2004;24:659-69.
26. Brandt K, Langhans W, Geary N, Leonhardt M. Beneficial and deleterious effects of hydroxycitrate in rats fed a high-fructose diet. Nutrition 2006;22:905-12.
27. Sharma H, Joshi A, Lad H, Bhatnagar D. Anti-oxidative, anti-inflammatory and anti-atherosclerotic effect of taurine on hypercholesterolemia induced atherosclerotic rats. Int J Pharm Pharm Sci 2018;10:145-50.
28. Kolodziejczyk J, Masullo M, Olas B, Piacente S, Wachowicz B. Effects of garcinol and guttiferone K isolated from Garcinia cambogia on oxidative/nitrative modifications in blood platelets and plasma. Platelets 2009;20:487-92.
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DATCHANAMURTY, B., M. K, and K. J. SALWE. “EVALUATION OF HYPOLIPIDEMIC ACTIVITY OF FRUIT RIND EXTRACTS OF GARCINIA GUMMIGUTTA IN DIET-INDUCED HYPERLIPIDEMIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 104-10, https://innovareacademics.in/journals/index.php/ajpcr/article/view/28201.
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