ROLE OF RADIATION AS EFFECTIVE INTERVENTION IN Aβ INDUCED OXIDATIVE STRESS IN ANIMAL MODEL OF ALZHEIMER'S DISEASE
Objective: The present study was undertaken to study the therapeutic effects of low dose fractionated cranial X-irradiation on reducing the amyloid-beta (AÎ²) induced oxidative stress burden in an animal model of Alzheimerâ€™s disease (AD).
Methods: S.D. female rats received an intracerebroventricular injection of AÎ² peptide at stereotaxically defined points. Experimental sessions were conducted by randomly dividing animals into four groups, namely sham-operated, AÎ²-injected, and AÎ² injection followed by cranial X-irradiation and only cranial X-irradiated. Anesthetized animals received 5 Î¼l synthetic AÎ² peptide injection with a 10 Î¼l Hamilton microsyringe with the needle kept in place for a period of 2min following injection. Sham-operated group received 5 Î¼l of bidistilled water instead of AÎ² peptide. Animals were treated 6 weeks post-surgery with fractionated radiation of 2Gy for 5 days. Neurobehavior studies were undertaken to confirm memory impairment along with biochemical indices involved in the antioxidant defense system.
Results: Fractionated cranial X-irradiation proved effective in restoration of activity of enzymes involved in the antioxidant defense system; the lipid peroxidation and catalase levels that showed a significant increase in AÎ²-treated group decreased on subsequent X-irradiation. Moreover, the decrease in the superoxide dismutase, glutathione, glutathione-S-transferase, and glutathione reductase levels witnessed an increase post-irradiation, implicating the X-irradiation to be an effective intervention to restore the redox status of the oxidatively stressed brain cells in AD condition.
Conclusion: The present study evaluated the therapeutic potential of low dose fractionated cranial X- irradiation by mitigating the amyloid-induced oxidative stress suggesting a novel treatment for AD-associated pathologies.
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