ANTICANCER ACTIVITY OF MICRO-ALGAE EXTRACT ON HUMAN CANCER CELL LINE (MG-63)

  • Jimcy James Department of Biotechnology, Algae Biomass Research Laboratory, School of Agriculture and Biosciences, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.
  • Jibu Thomas Department of Biotechnology, Algae Biomass Research Laboratory, School of Agriculture and Biosciences, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.

Abstract

Objective: The objective of the present study is to assess the anticancer effect of limnic algae extract of KACC 2 (Karunya Algae Culture Collection) and KACC 23 against MG-63 cell line.

Methods: Bioactive compounds were extracted from the algae culture by Soxhlet extraction method using methanol as solvent, and the extract was checked for its anticancer activity. Polyphenols were estimated using Folin-Ciocalteu reagent method. The constituents of the extract were analyzed using gas chromatography-mass spectrometry (GC-MS).

Results: The results showed that KACC 2 had more phenolic content than KACC 23. The half maximal inhibitory concentration value for KACC 2 and 23 was found as 0.386 μl/ml and 0.285 μl/ml of extracts necessary for the 50% of cell death. GC-MS analysis revealed that the compounds with possible therapeutic effects are quercetin, stigmast-4-en-3-one, epoxygedunin, which has got anticancer activity.

Conclusion: These observations point out that the algae extract of KACC 2 and KACC 23 has anticancer property and the phytoconstituents contributed to the anticancer effects.

Keywords: Microalgae, Anticancer, MG-63 cell line, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Gas chromatography-mass spectrometry, Polyphenol.

References

1. Villarreal-Gómez LJ, Soria-Mercado IE, Guerra-Rivas G, Ayala-Sánchez NE. Antibacterial and anticancer activity of seaweeds and bacteria associated with their surface. Rev Biol Mar Oceanogr 2010;45:267-75.
2. Prasad MP, Sushant S, Chikkaswamy BK. Phytochemical analysis, antioxidant potential, antibacterial activity and molecular characterization of Clerodendrum species. Int J Mol Biol 2012;3:71-6.
3. Murugesan SU, Bhuvaneswari SU, Sivamurugan VA. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9:192-7.
4. Kumar RV, Murugesan S, Bhuvaneswari S. Phytochemical analysis of red alga Champia parvula (C. Agardh) collected from Mandapam coast of Tamil Nadu, India. Int J Adv Pharm Res 2015;4:15-20.
5. Bayala B, Bassole IH, Scifo R, Gnoula C, Morel L, Lobaccaro JM, et al. Anticancer activity of essential oils and their chemical components-a review. Am J Cancer Res 2014;4:591-607.
6. Kumar KS, Ganesan K, Rao PS. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty-an edible seaweed. Food Chem 2008;107:289-95.
7. Amruthraj NJ, Raj-Preetam JP, Saravanan S, Lebel-Antoine L. In vitro studies on anticancer activity of capsaicinoids from Capsicum chinense against human hepatocellular carcinoma cells. Int J Pharm Pharm Sci 2014;6:254-558.
8. Ashwini S, Babut S, Saritha MS. Seaweed extracts exhibit anticancer activity against HeLa cell lines. Int J Curr Pharm Res 2016;9:114-7.
9. Varalakshmi B, Anand AV, Karpagam T, Bai JS, Manikandan R. In vitro antimicrobial and anticancer activity of Cinnamomum zeylanicum Linn bark extracts. Int J Pharm Pharm Sci 2014;6:12-8.
10. Kavitha J, Palani S. Phytochemical screening, GC-MS analysis and antioxidant activity of marine algae Chlorococcum humicola. World J Pharm Pharm Sci 2016;5:1154-67.
11. Abdel-Aal EI, Haroon AM, Mofeed J. Successive solvent extraction and GC-MS analysis for the evaluation of the phytochemical constituents of the filamentous green alga Spirogyra longata. Egypt J Aquat Res 2015;41:233-46.
12. Zendehbad SH, Mehran MJ, Malla SU. Flavonoids and phenolic content in wheat grass plant (Triticum aestivum). Asian J Pharm Clin Res 2014;7:184-7.
13. Sharma RA, Chandan GO, Chahal AN, Saini RV. Antioxidant and anticancer activity of methanolic extract from Stephania elegans. Int J Pharm Pharm Sci 2017;9:245-9.
14. Yuan YV, Walsh NA. Antioxidant and anti-proliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol 2006;44:1144-50.
15. Patel S, Gheewala N, Suthar A, Shah A. In-vitro cytotoxicity activity of Solanum nigrum extract against HeLa cell line and Vero cell line. Int J Pharm Pharm Sci 2009;1:38-46.
16. Jayasree P, Thiruchelvi R, Balashanmugam P. Evaluation of antibacterial, antioxidant, and anticancer potentials from marine red algae Gracilaria corticata. Asian J Pharm Clin Res 2018;11:347-50.
17. Garai S, Ghosh R, Bandopadhyay PP, Mandal NC, Chattopadhyay A. Anti-microbial and anti-cancer properties of echinocystic acid extracted from Luffa cylindrica. J Food Process Technol 2018;9:717.
18. Ignat I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem 2011;126:1821-35.
19. Lemieux JM, Wu G, Morgan JA, Kacena MA. DMSO regulates osteoclast development in vitro. In Vitro Cell Dev Biol Anim 2011;47:260-7.
20. Pereira TB, Rocha E Silva LF, Amorim RC, Melo MR, Zacardi de Souza RC, Eberlin MN, et al. In vitro and in vivo anti-malarial activity of limonoids isolated from the residual seed biomass from Carapa guianensis (andiroba) oil production. Malar J 2014;13:317.
21. Dose J, Matsugo S, Yokokawa H, Koshida Y, Okazaki S, Seidel U, et al. Free radical scavenging and cellular antioxidant properties of astaxanthin. Int J Mol Sci 2016;17:103.
22. Rosenberg OA, Seiliev AA, Shulga AE, Zhuikov AG, Volchkov VA. Prolongation of anti-inflammatory activity of glucocorticosteroids encapsulated in large oligolamellar liposomes in treatment of arthritis in rabbits. Int J Biomed 2017;7:131-4.
23. Juvale K, Stefan K, Wiese M. Synthesis and biological evaluation of flavones and benzoflavones as inhibitors of BCRP/ABCG2. Eur J Med Chem 2013;67:115-26.
24. Zhang Q, Zhang J, Shen J, Silva A, Dennis DA, Barrow CJ. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. J Appl Phycol 2006;18:445-50.
Statistics
83 Views | 140 Downloads
How to Cite
James, J., and J. Thomas. “ANTICANCER ACTIVITY OF MICRO-ALGAE EXTRACT ON HUMAN CANCER CELL LINE (MG-63)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 139-42, doi:10.22159/ajpcr.2019.v12i1.28652.
Section
Original Article(s)