IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL

Authors

  • Deena Priscilla H Department of Biotechnology, Bishop Heber College, Tiruchirappalli - 620 017, Tamil Nadu, India.
  • Jasmine R Department of Biotechnology, Bishop Heber College, Tiruchirappalli - 620 017, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.28667

Keywords:

Terminalia chebula, Antioxidant, Apoptosis, Hemolysis

Abstract

Objective: Antioxidants are very important for human health, and hence, external supplementation is recommended to protect cells from the deleterious effects of excessive reactive oxygen species conditions that are capable of causing direct damage to deoxyribonuclieic acid (DNA) which might lead to the cause of cancer. In our research work, we have aimed to evaluate the antioxidant potential of Terminalia chebula and pyrogallol to relate to its apoptotic nature as well as prove its non-toxicity.

Methods: Investigations were carried out to determine the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and lipid peroxidation inhibition assay of the ethanolic extract of T. chebula and pyrogallol. The apoptotic nature of tumor cells was determined by comet assay. To prove the non-toxicity of the extract as well as compound, the hemolysis assay was carried out.

Results: The results of our study established the antioxidant potential of the plant which may serve as additional evidence for the antiproliferative activity of the plant. It is considered safe for human use.

Conclusion: Plant-based traditional medicine system continues to play an essential role in health care. The ethanolic extract of T. chebula and pyrogallol possesses antioxidant and anticancer properties.

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References

Bag A, Bhattacharyya SK, Chattopadhyay RR. The development of Terminalia chebula Retz. (Combretaceae) in clinical research. Asian Pac J Trop Biomed 2013;3:244-52.

Gacche RN, Shaikh RU, Pund MM. In vitro evaluation of anticancer and antimicrobial activity of selected medicinal plants from Ayurveda. Asian J Trad Med 2011;6:1-7.

Umadevi M, Kumar KP, Bhowmik D, Duraivel S. Traditionally used anticancer herbs in India. J Med Plants Stud 2013;1:56-74.

Chen C, Chen Y, Lu P, Lin W, Chen T, Lin C. Proteus mirabilis urinary tract infection and bacteremia: Risk factors, clinical presentation, and outcomes. J Microbiol Immunol Infect 2012;45:228-36.

Walia H, Arora S. Terminalia chebula - A pharmacognostic account. J Med Plants Res 2013;7:1351-61.

Mukherjee PK. Quality Control of Herbal Drugs: An Approach to the Evaluation of Botanicals. New Delhi: Business Horizons; 2002.

Sánchezmoreno C, Larrauri JA, Saura-Calixto F. A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 1998;76:270-6.

Botsoglou NA, Fletouris DJ, Papageorgiou GE, Vassilopoulos VN, Mantis AJ, Trakatellis AG. Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food, and feedstuff samples. J Agric Food Chem 1994;42:1931-7.

Nandhakumar S, Parasuraman S, Shanmugam M, Rao K, Chand P, Bhat B. Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay). J Pharmacol Pharmacother 2011;2:107.

Xianngou H, Ursula M. Antifungal compound from Solanum nigrescens. J Ethano Pharmacol 1994;43:173-7.

Priscilla HD, Jasmine R. Evaluation of in vitro anticancer activity of Terminalia chebula and identification of phytocompounds by GC-MS analysis. J Chem Pharm Res 2016;8:683-8.

Baliah NT, Astalakshmi A. Phytochemical analysis and antibacterial activity of extracts from Terminalia chebula Retz. Int J Curr Microbiol Appl Sci 2014;3:992-9.

Guleria S, Dev K, Khosla PK. Comparative analysis of phytochemicals and antioxidant activities of fruit and leaves of Terminalia chebula from Himachal Pradesh. Int J Biol Pharm Allied Sci 2016;5:1195-206.

Subha SS, Divakar KM. GC-MS analysis of the phytoconstiutents of methanolic fruit extracts of Terminalia chebula Retz. Int J Innov Pharm Sci Res 2016;4:53-61.

Akmal NL, Gayathri R, Priya VV. Phytochemical screening and in vitro free radical scavenging of Terminalia chebula. Int J Pharm Sci Rev Res 2016;39:226-31.

Tariq AL, Riyaz AL. Significance and importance of phytochemicals present in T. chebula. Int J Drug Res 2013;5:256-62.

Ram J, Moteriya P, Chanda S. Phytochemical screening and reported biological activities of some medicinal plants of Gujarat region. J Pharmacogn Phytochem 2015;41:92-1988.

Devi PN, Kaleeswari S, Poonkothai M. Antimicrobial activity and phytochemical analysis of fruit extracts of Terminalia bellerica. Int J Pharm Pharm Sci 2014;6:639-42.

Manohar VR, Chandrashekar R, Rao SN. Phytochemical analysis of Terminalia belerica fruit pulp extracts. World J Pharm Pharm Sci 2012;1:1376-83.

Tinh TH, Nuidate T, Vuddhakul V, Rodkhum C. Antibacterial activity of pyrogallol, a polyphenol compound against Vibrio parahaemolyticus isolated from the central region of Thailand. Proc Chem 2016;18:162-8.

Karimi E, Mehrabanjoubani P, Keshavarzian M, Oskoueian E, Jaafar HZ, Abdolzadeh A. Identification and quantification of phenolic and flavonoid components in straw and seed husk of some rice varieties (Oryza sativa L.) and their antioxidant properties. J Sci Food Agric 2014;94:2324-30.

Nemec MJ, Kim H, Marciante AB, Barnes RC, Talcott ST, Talcott SU. Pyrogallol, an absorbable microbial gallotannins-metabolite and mango polyphenols (Mangifera indica L.) suppress breast cancer ductal carcinoma in situ proliferation in vitro. Food Function 2016;7:3825-33.

Haritha K, Ramesh B, Saralakumari D. Effect of Aloe vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats. J Acute Med 2014;4:38-44.

Chioma OO, Victor OG, Emeka AG. Ethanol extracts of Piper nigrum ameliorate serum lipid peroxidation and antioxidant enzyme activities in diabetic rats. World Appl Sci J 2014;32:27-33.

Hefnawy T, Ali O. Assessment of antioxidant capacity of ethanolic extract of Portula caoleracea leaves in vitro and in vivo. J Med Plants Res 2015;9:335-42.

Piluzza G, Bullitta S. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. Pharm Biol 2011;49:240-7.

Govindan P, Muthukrishnan S. Evaluation of total phenolic content and free radical scavenging activity of Boerhavia erecta. J Acute Med 2013;3:103-9.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118.

Vijaya Bharathi S, Anuradha V, Rubalakshmi G. In vitro antioxidant and antimicrobial activity of Phoenix pusilla root extract. Asian J Pharm Clin Res 2018; 11:194-7.

Loganayaki N, Siddhuraju P, Manian S. Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. J Food Sci Technol 2011;50:687-95.

Fattahi S, Zabihi E, Abedian Z, Pourbagher R, Ardekani AM, Mostafazadeh A, et al. Total phenolic and flavonoid contents of aqueous extract of stinging nettle and in vitro antiproliferative effect on Hela and BT-474 cell lines. Int J Mol Cell Med 2014;3:102-7.

Chang CL, Lin CS. Phytochemical composition, antioxidant activity, and neuroprotective effect of Terminalia chebula Retzius extracts. Evid Based Complementary Altern Med 2012;2012:1-7.

Tor YS, Yazan LS, Foo JB, Armania N, Cheah YK, Abdullah R, et al. Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa. BMC Complementary Altern Med 2014;14:55.

Bag A, Bhattacharyya SK, Pal NK, Chattopadhyay RR. In vitro antimicrobial potential of Terminalia chebula fruit extracts against multidrug-resistant uropathogens. Asian Pac J Trop Biomed 2012;2:597-1.

Sharma S, Kumar V. In vitro cytotoxicity effect on MCF-7 cell line of co-encapsulated artesunate and curcumin liposome. Int J Pharm Pharm Sci 2017;9:123-8.

Published

07-01-2019

How to Cite

Priscilla H, D., and J. R. “IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 1, Jan. 2019, pp. 212-5, doi:10.22159/ajpcr.2019.v12i1.28667.

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