EVALUATION OF SIRTUIN 3 BIOMARKER BEFORE AND AFTER EXERCISE REGIMEN IN CHRONIC UNPREDICTABLE MILD STRESS-INDUCED DEPRESSED RATS
Objective: Depression is one of the most common psychiatric illnesses that cause disability and public health problems. Physical exercise has been shown to reduce the stress impairment and ameliorate depressive symptoms. The objectives of the present study were to confirm, whether swimming exercise alleviates the depression-like behavior induced by chronic unpredictable mild stress (CUMS) through the alteration in the expression of sirtuin 3 (sirt 3) in brain.
Methods: This study was undertaken to evaluate the possible antidepressant activity of exercise. Female Wistar rats weighing about 120â€“150 g were divided into four groups of six animals in each: Control, CUMS-induced, venlafaxine-treated, and exercise-treated groups. The rat model of depression was established using CUMS protocol. Behavioral assessment was performed by sucrose consumption test and forced swim test. Semi-quantitative reverse transcription-polymerase chain reaction and Western blot analysis were performed to study the gene expression and the protein activity of SIRT3.
Results: Our results showed that the gene expression and protein activity of sirt3 were found to be significantly increased in the exercise-treated group when compared to the depressed group.
Conclusion: The interesting fact from this study is that swimming exercise increases sirt3 expression and possesses a beneficial effect in curing depression.
2. Pamulaparthi A, Prathap VR, Banala M, Nanna RS. Experimental evaluation of antidepressant and antianxiety activities of aqueous leaf extracts of Senna alata (L.) Roxb. using in vitro animal models. Int J Curr Pharm Res 2016;8:60-3.
3. Paul EH, Charles BN. Advances in the treatment of depression. Neurotherapeutics 2006;3:42.
4. Pati D, Pandey DK, Mahesh R, Vand K, Jhadev HR. Antidepressant-like activity of Mucuna pruriens: A traditional indian herb in rodent models of depression. Pharmacol Online 2010;1:537-51.
5. Sarandol A, Sarandol E, Eker SS, Erdine S, Vatansever E, Kirli S. Major depressive disorder is accompanied with oxidative stress: short-term antidepressant treatment does not alter oxidative-antioxidative systems. Human Psycho Pharmacol Clin Exp 2007;22:67-73.
6. Eren M, Naziroglu M, Demirdas A, Celik O, Uguz AC, Altunbasak A, et al. Venlafaxine modulates depression induced oxidative stress in brain and medulla of rat. Neurochem Res 2007;32:497-505.
7. Tiwari P, Pratap SR, Ritesh J, Misha R. Antidepressant activity of hydroalcoholic extract of Zingiber officinale. Int Res J Pharm 2012;3:149-51.
8. Michan S, Sinclair D. Sirtuins in mammals: Insights into their biological function. Biochem J 2007;404:1-13.
9. Saunders LR, Verdin E. Sirtuins: Critical regulators at the crossroads between cancer and aging. Oncogene 2007;26:5489-504.
10. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward JL, et al. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1alpha in skeletal muscle. Aging 2009;15:771-83.
11. Finck BN, Kelly DP. PGC-1 coactivators: Inducible regulators of energy metabolism in health and disease. J Clin Invest 2006;116:615-22.
12. Handschin C, Spiegelman BM. Peroxisome proliferator activated receptor (gamma) coactivator 1 coactivators, energy homeostasis and metabolism. Endocr Rev 2006;27:728-35.
13. Castren E, Rantamaki T. The role of BDNF and its receptors in depression and antidepressant drug action: Reactivation of developmental plasticity. Dev Neurobiol 2010;70:289-97.
14. Willner P. Validity, reliability and utility of the chronic mild stress model of depression: A 10-year review and evaluation. Psychopharmacology (Berl) 1997;134:319-29.
15. Redrobe JP, Bourin M, Colombel MC, Baker GB. Dose-dependent noradrenergic and serotonrgic properties of venlafaxine in animal models indicative of antidepressant activity. Psychopharmacology 1998;138:1-8.
16. Greenwood BN, Fleshner M. Exercise, stress resistance, and central serotonergic systems. Exerc Sport Sci Rev 2011;39:140-9.
17. Paluska SA, Schwenk TL. Physical activity and mental health: Current concepts. Sports Med 2000;29:167-80.
18. Lin TW, Kuo YM. Exercise benefits brain function: The monoamine connection. Brain Sci 2013;3:39-53.
19. Strawbridge WJ, Deleger S, Roberts RE, Kaplan GA. Physical activity reduces the risk of subsequent depression for older adults. Am J Epidemiol 2002;156:328-34.
20. Nirmal J, Babu CS, Harisudhan T, Ramanathan M. Evaluation of behavioral and antioxidant activity of Cytisus scoparius Link in rats exposed to chronic unpredictable mild stress. BMC Complement Altern Med 2008;8:15.
21. Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: A primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229:327-36.
22. Borah A, Singha B, Phukan S. Antidepressant effect of ceftriaxone in forced swimming test and in tail suspension test in mice. Int J Pharm Pharm Sci 2016;8:191-4.
23. Lin J, Wu PH, Tarr PT, Lindenberg KS, St-Pierre J, Zhang CY, et al. Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice. Cell 2004;119:121-35.
24. Liu X, Yanj LJ, Fan SJ, Jiang H, Pan F. Swimming exercise effects on the expression of HSP70 and iNOS in hippocampus and prefrontal cortex in combined stress. Neurosci Lett 2010;476:99-103.
25. Contarteze RV, Fde BM, Gobatto CA, Mello MA. Stress biomarkers in rats submitted to swimming and treadmill running exercises. Comp Biochem Physiol A Mol Integr Physiol 2008;151:415-22.
26. Noonan KE, Beck C, Holzmayer TA, Chin JE, Wunder JS, Andrulis IL, et al. Quantitative analysis of MDRI (multidrug resistance) gene expression inhuman tumors by polymerase chain reaction. Proc Natl Acad Sci 1990;87:7160-4.
27. Kinoshita T, Imamura J, Nagai H, Shimotohno K. Quantification of gene expression over a wide range by the polymerase chain reaction. Anal Biochem 1992;206:231-5.
28. Gold PW, Chrousos GP. Organization of the stress system and its dysregulation in melancholic and atypical depression high vs low CRH/NE stress. Mol Psychiatry 2002;7:254-75.
29. Willner P, Muscat R, Papp M. Chronic mild stress-induced anhedonia: A realistic animal model of depression. Neurosci Biobehav Rev 1992;16:525-34.
30. Patel SS, Rajshree N, Shah PV. Evaluation of antidepressant activity of herbomineral formulation. Int J Pharm Pharm Sci 2016;8:145-7.
31. Kalueff AV, Gallagher PS, Murphy DL. Are serotonin transporter knockout mice depressed?: Hypoactivity but in anhedonia. Neuroreport 2006;17:1347-51.
32. Strekalova T, Gorenkova N, Schunk E, Dolgov O, Bartch D. Selective effects of citalopram in a mouse of stress-induced anhedonia with a control for chronic stress. Behav Pharmacol 2006;17:271-87.
33. Willner P. The Validity of animal models of depression. Psychopharmacology 1984;83:1.
34. Cheng A, Yang Y, Zhou Y, Maharana C, Lu D, Peng W, et al. Mitochondrial SIRT3 mediates adaptive responses of neurons to exercise and metabolic and excitatory challenges. Cell Metab 2015;23:128-42.
35. Song W, Song Y, Kincaid B, Bossy B, Bossy-Wetzel E. Mutant SOD1G93A triggers mitochondrial fragmentation inspinal cord motor neurons: Neuroprotection by SIRT3 and PGC1Î±. Neurobiol Dis 2013;51:72-81.
36. Wier HJ, Murray TK, Kehoe PG, Love S, Verdin EM, Oâ€™Neill MJ, et al. CNS SIRT3 expression is altered by reactive oxygen species and in Alzheimerâ€™s disease. PLoS One 2012;7:e48225.
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