BIOFABRICATION OF ZINC OXIDE NANOPARTICLES USING PTEROCARPUS MARSUPIUM AND ITS BIOMEDICAL APPLICATIONS
Objective: The objective of the study is to perform the synthesis of zinc oxide nanoparticles using the bark extract of Pterocarpus marsupium and to evaluate its biomedical applications.
Methods: Various concentrations of zinc acetate are used, and synthesis conditions were optimized to get a stable nanoparticle. The finest synthesis condition for zinc oxide nanoparticle production was at pH 7 with 20 ml extract, zinc acetate 10 mM, and 120 min of reaction time. The synthesized nanopowder was characterized using various analytical techniques, such as ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The synthesized nanoparticles were tested for their antimicrobial, anti-inflammatory, inhibition of lipid peroxidation, and inhibition of amylase activity.
Results: The size range of nanoparticles obtained was in the range of 10â€“32 nm as reported by SEM. The UV-visible absorption spectrum of the synthesized nanoparticle showed a peak at 340 nm, which confirmed the presence of nanoparticles. FTIR spectroscopy analysis indicated the presence of zinc oxide stretching at 666.22 cm-1. Further, the IR spectra indicated the presence of alcohols and acids, which can act as capping agents around the nanoparticles. XRD analysis confirmed the crystalline nature of nanoparticles.
The synthesized nanoparticle showed appreciable antimicrobial activity. Zinc oxide nanoparticles at 40 Î¼g/well were tested against phytopathogens, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus flavus, and Aspergillus niger showed 16, 13, 15, and 16 mm zones of inhibition, respectively. The synthesized nanoparticle showed a considerable increase in inhibition of lipid peroxidation and amylase activity. The nanoparticle also exhibited appreciable anti-inflammatory activity measured by the inhibition of albumin denaturation.
Conclusion: The study instigates the simple and convenient method of synthesizing zinc oxide nanoparticles using P. marsupium and its few biomedical applications.
2. Wang Y, Herron N. Nanometer-sized semiconductor clusters: Materials synthesis, quantum size effects, and photophysical properties. J Phys Chem 1991;95:525-32.
3. Schmid G. Large clusters and colloids. Metals in the embryonic state. Chem Rev 1992;92:1709-27.
4. Hoffman AJ, Mills G, Yee H, Hoffmann M. Q-sized cadmium sulfide: Synthesis, characterization, and efficiency of photoinitiation of polymerization of several vinylic monomers. J Phys Chem 1992;96:5546-52.
5. Abhishek N, Hegde K. Pharmacological profile of Pterocarpus marsupium with a note on its therapeutic activity. Int J Pharm Clin Res 2017;3 Suppl 1:32-7.
6. Manikani KL, Krishna V, Manjunatha BK, Vidya SM, Singh SD, Manohara YN, et al. Evaluation of hepatoprotective activity of stem bark of Pterocarpus marsupium Roxb. Indian J Pharmacol 2005;37 Suppl 3:165-8.
7. Vats V, Yadav SP, Biswas NR, Grover JK. Anti-cataract activity of Pterocarpus marsupium bark and Trigonella foenum-graecum seeds extract in alloxan diabetic rats. J Ethnopharmacol 2004;93 Suppl 2-3:289-94.
8. Hougee S, Faber J, Sanders A, Dejong RB, Vandenberg WB, Garssen J, et al. Selective COX-2 inhibition by a Pterocarpus marsupium extract characterized by pterostilbene, and its activity in healthy human volunteers. Planta Med 2005;71 Suppl 5:387-92.
9. Gnanasangeetha D, Thambavani SD. Biogenic production of zinc oxide nanoparticles using Acalypha indica. J Chem Biol Phys Sci 2014;4 Suppl 1:238-46.
10. Kudi AC, Umoh JU, Eduvic LO, Getu J. Screening of some Nigerian medicinal plants for antibacterial activity. J Ethnopharmacol 1999;67:225-8.
11. Vlietink AJ, Van Hoof L, Totte J, Laure H, Vanden Berhe D, Rwangabo PC, et al. Screening of hundred rwandese medical plants for antimicrobial and antiviral properties. J Ethnopharmacol 1995;46:31-47.
12. Ruberto G, Baratta MT, Deans SG, Dorman HJ. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med 2000;66:687-93.
13. Bernfeld P. Amylases, Î± and Î². Methods Enzymol 1955;1:149-58.
14. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum preoteins,especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-173.
15. Varghese E, George M. Green synthesis of zinc oxide nanoparticles. Int J Adv Res Sci Eng 2015;4 Suppl 1:307-14.
16. Djaja N, Montja D, Saleh R. The effect of Co incorporation into ZnO nanoparticles. Adv Mater Phys Chem 2013;3 Suppl 1:33-41.
17. HernÃ¡ndez A, Maya L, SÃ¡nchez-Mora E, SÃ¡nchez EM. Sol-gel synthesis, characterization and photocatalytic activity of mixed oxide ZnO-Fe2O3. J Solgel Sci Technol 2007;42 Suppl 1:71-8.
18. Zhou J, Zhao F, Wang Y, Zhang Y, Yang L. Size-controlled synthesis of ZnO nanoparticles and their photoluminescence properties. J Lumin 2007;122-3 Suppl 1-2:195-7.
19. Khoshhesab ZM, Sarfaraz M, Asadabad MA. Preparation of zno nanostructures by chemical precipitation method. Synth React Inorg Met Org Nano Met Chem 2011;41 Suppl 7:814-9.
20. Raj LF, Jayalakshmy E. A biogenic approach for the synthesis and characterization of zinc oxide nanoparticles produced by Tinospora cordifolia. Int J Pharm Pharm Sci 2015;7 Suppl 8:384-6.
21. Fowsiya J, Madhumitha G, Al-Dhabi NA, Arasu MV. Photocatalytic degradation of Congo red using Carissa edulis extract capped zinc oxide nanoparticles. J Photochem Photobiol B 2016;162:395-401.
22. Dobrucka R, DÅ‚ugaszewska J. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. Saudi J Biol Sci 2016;23:517-23.
23. Deepa R, Manjunatha H, Krishna V, Swamy BE. Evaluation of antimicrobial activity and antioxidant activity by electrochemical method of ethanolic extract of Pterocarpus marsupium Roxb bark. J Biotechnol Biomater 2014;4:166-9.
24. Sirelkhatim A, Mahmud S, Seeni A, Kaus NH, Ann LC, Bakhori SK, et al. Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Nano Micro Lett 2015;7 Suppl 3:219-42.
25. Hodges DM, DeLong JM, Forney CF, Prange RK. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 1999;207:604-11.
26. Musa MY, Griffith AM, Michels AJ, Schneider E, Frei B. Inhibition of Î±-amylase and Î±-glucosidase activity by tea and grape seed extracts and their constituent catechins. J Agric Food Chem 2012;60:8924-9.
27. Dhobale S, Thite T, Laware SL, Rode CV, Koppikar SJ, Ruchika-Kaul G, et al. Zinc oxide nanoparticles as novel alpha-amylase inhibitors. J App Phys 2008;104:94907.
28. Leelaprakash G, Dass SM. In vitro antiinflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2010;3:189-96.
29. Ingle PV, Patel DM. C-reactive protein in various disease condition-an overview. Asian J Pharm Clin Res 2011;4 Suppl 1:9-13.
30. Phongpradist R, Chaiyana W, Anuchapreeda S. Curcumin-loaded multi-valent ligands conjugated-nanoparticles for anti-inflammatory activity. Int J Pharm Pharm Sci 2015;7 Suppl 4:203-8.
31. Sangeetha G, Vidhya R. In vitro anti-inflammatory activity of different parts of Pedalium murex (L.) Int J Herb Med 2016;4 Suppl 3:31-6.
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