THE CHEMOADJUVANT POTENTIAL OF PERGULARIA DAEMIA (FORSK.) ON EXPRESSION PATTERN IN EXPERIMENTAL ORAL CARCINOGENESIS

Authors

  • Sankaran Mirunalini
  • Kandhan Karthishwaran
  • Velusamy Vaithiyanathan

Abstract

Abstract

Pergularia daemia (Family: Asclepiadaceae), is a perennial herb growing widely along the road sides of India. Traditionally, the plant is utilized in many disease conditions. These findings provide valuable information that might help in the selection of possible biomarkers that can be used in early detection of the alarming problem of oral cancer in Southeast Asia. Topical application of DMBA for 14 weeks induced buccal pouch tumours that showed aberrant expression of cytokeratins, a marker for epithelial carcinomas. This was associated with increased cell proliferation and evasion of apoptosis as revealed by upregulation of proliferating cell nuclear antigen, NF-κB, mutant p53, Bcl-2 and downregulation of Bax and caspase 3 protein expression. Our findings clearly suggest that PDME may play a role as a novel chemopreventive or therapeutic agent for oral carcinogenesis.

Key words: Pergularia daemia, Chemoadjuvant, RT-PCR, Western Blotting

References

Tsantoulis PK, Kastrinakis NG, Tourvas AD, Laskaris G, Gorgoulis VG. 2007. Advances in the biology of oral cancer. Oral Oncology 43: 523– 534.

Jemal A, Murray T, Ward E. 2005. Cancer statistics. CA Cancer J Clin 55: 10-30.

Fenley J, Bray F, Pisani D Me. 2000. World Health Organization. GLOBOCAN; Cancer incidence, mortality and prevalence worldwide. Lyon France: IARC.

Parkin DM, Muir CS, Whelan SL. 2002. Cancer Incidence in Five Continents, IARC Scientific Publications, Lyon. 7: 155.

Linkov F, Lisovich A, Yurkovetsky Z, Marrangoni A, Velikokhatnaya L, Nolen B, Winans M, Bigbee W, Siegfried J, Lokshin A, Ferris RL. 2007. Early detection of head and neck cancer: development of a novel screening tool using multiplexed immunobead-based biomarker profiling. Cancer Epidemiol Biomarkers Prev. 16:102-7.

Golub TR, Slonim DK, Tamayo H Gaasenbeek CM, Mesirov JP, Coller H, Loh ML, Downing JR, Caligiuri MA, Bloomfield CD, Lander ES. 1999. Molecular Classification of Cancer: Class Discovery and Class Prediction by Gene Expression Monitoring. Sci 286: 531-537.

Nagini S. 2009. Of humans and hamsters: the hamster buccal pouch carcinogenesis model as a paradigm for oral oncogenesis and chemoprevention. Anticancer Agents Med Chem. 9: 843-52.

Gimenez-Conti IB, Slaga TJ. 1992. The hamster cheek pouch model for carcinogenesis and chemoprevention. Adv Exp Med Biol 320: 63-67.

Shkler G. 1998. Mechanism of cancer inhibition by antioxidant nutrients. Oral Oncol 34: 24-29.

Karthishwaran K and Mirunalini S. 2010. Therapeutic potential of Pergularia daemia (Forsk.): The ayurvedic wonder. Int J Pharmacol. 6: 836-843.

Nongyao S and Kitja S. 2005. The effects of extracts from anti-diarrheic Thai medicinal plants on the in vitro growth of the intestinal protozoa parasite: Blastocystis hominis. J Ethnopharmacol. 98: 67–72.

Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 76: 4350–4354.

Chomczynski P, Sacchi N. 1987. "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Anal Biochem. 162: 156–159.

Miyata M, Furukawa M, Takahasi K, Gonzalez FJ, Yamazoe Y. 2001. Mechanism of 7, 12–dimethylbenz(a) anthracene induced immunotoxicity: Role of Metabolic activation at the target organ. Jpn J pharmacol 86: 302-309.

Young JS, Kyung SC, Hyun HC, Seong SH, Young SK, Kwang KP, Sang SL. 2001. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kB activation. Mutation Research. 480: 243–268.

Chen YJ, Jin YT, Shieh DB, Tsai ST, Wu LW. 2002. Molecular characterization of angiogenic properties of human oral squamous cell carcinoma cells. Oral Oncol 38: 699-705.

Stoimenov I, Helleday T. 2009. PCNA in the crossroad of cancer. Biochem Soc Trans 37: 605–13.

Staibano S, Mignogna MD, Lo Muzio L. 1998. Over expression of cyclin-D1, Bcl-2, and Bax proteins, proliferating cell nuclear antigen (PCNA), and DNA-ploidy in squamous cell carcinoma of the oral cavity. Hum Pathol 29: 1189–94.

Qin LF, Ng IO, Fan ST, Ng M. 1998. p21℠WAF1, p53and PCNA expression and p53 mutation status in hepatocel-lular carcinoma. Int J Cancer 79: 424–8.

Singh S, Aggarwal BB. 2005. Activation of transcription factor NF-kB is suppressed by curcumin (Diferuloylmethane), J Biol Chem 270: 24995–25000.

Akao Y, Otsuki Y, Kataoka S, Ito Y, Tsujimoto Y. 1994. Multiple subcellular localization of bcl-2: detection in nuclear outer membrane, endoplasmic reticulum membrane and mitochondrial membranes. Cancer Res 54: 2468-2471.

Vaux DL, Cory S, Adams JM. 1998. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature 335:440-442.

Shalmali P, Nagashri MN, Gopinath KS, Arun K. 2011. Expression Profiling of CYP1B1 in Oral Squamous Cell Carcinoma: Counterintuitive Downregulation in Tumors. PLos One 6:27914-27919.

Sissung TM, Price DK, Sparreboom A, Figg WD. 2006. Pharmacogenetics and regulation of human cytochrome P450 1B1: implications in hormone-mediated tumor metabolism and a novel target for therapeutic intervention. Mol Cancer Res 4: 135–150.

Hunter T, Pines J. 1994. Cyclins and cancer. II. Cyclin D and CDK inhibitors come of age. Cell 79: 573–582.

Published

2013-08-01

How to Cite

Mirunalini, S., K. Karthishwaran, and V. Vaithiyanathan. “THE CHEMOADJUVANT POTENTIAL OF PERGULARIA DAEMIA (FORSK.) ON EXPRESSION PATTERN IN EXPERIMENTAL ORAL CARCINOGENESIS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 7, Aug. 2013, pp. 91-97, https://innovareacademics.in/journals/index.php/ajpcr/article/view/287.

Issue

Section

Articles