IN VITRO ANTIMICROBIAL ACTIVITY, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC SEED EXTRACT OF FICUS BENGHALENSIS LINN

Authors

  • Vimala G Department of Zoology, Arignar Anna Govt. Arts College for Women, Vellore, Tamil Nadu, India.
  • Gricilda Shoba F PG & Research Department of Zoology, Voorhees College, Vellore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.28818

Keywords:

Ficus benghalensis, High-performance thin-layer chromatography, Gas chromatography-mass spectrometry, Antimicrobial, Herbal drug

Abstract

Objective: In the traditional medicine, Ficus benghalensis is used for the treatment of ulcer, diabetes, inflammation, liver disorders, urinary disorders, vomiting, vaginal complains, and fever and as a bitter tonic. However, despite its traditional usage as an antimicrobial agent, there is no information regarding its effectiveness in infections caused by pathogenic microbes. Hence, we evaluated the ethanolic extract of the seeds of F. benghalensis for its antimicrobial activity.

Methods: The antimicrobial activity of the extract at five different concentrations was tested against few common human pathogenic microorganisms by agar disc diffusion assay. Streptomycin (10 μg/ml) and Amphotericin B (10 μg/ml) were used as standards for antibacterial and antifungal studies, respectively. Few phenolic compounds were identified by standard high-performance thin-layer chromatography (HPTLC) and gas chromatography-mass spectrometry (GC-MS) techniques.

Results: The zone of inhibition was extremely great for Pseudomonas aeruginosa (19 mm), Enterococcus faecalis (18 mm), and Aspergillus niger (13 mm). Moreover, through HPTLC analysis, few phenolic compounds such as quercetin (Rf value - 0.54), gallic acid (Rf value - 0.45), and tannic acid (Rf value - 0.45) were identified. A total of seven compounds were identified in the extract using GC-MS analysis.

Conclusions: The results, therefore, clearly indicate that the crude extract from F. benghalensis seeds could be used as a potential source of natural antimicrobial agent due to the presence of the phytoconstituent quercetin, gallic acid, and tannic acid in abundance along with other active compounds and support the traditional use of the plant in the treatment of infections.

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Author Biographies

Vimala G, Department of Zoology, Arignar Anna Govt. Arts College for Women, Vellore, Tamil Nadu, India.

Associate Professor and Research Supervisor in the Department of Zoology

Gricilda Shoba F, PG & Research Department of Zoology, Voorhees College, Vellore, Tamil Nadu, India.

Department of Zoology

References

Namita P, Mukesh R. Medicinal plants used as antimicrobial agents: A review. Int Res J Pharm 2012;3:31-40.

Gootz TD. The global problem of antibiotic resistance. Crit Rev Immunol 2010;30:79-93.

Abreu AC, Mc Bain AJ, Simoes M. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep 2012;7:1007-21.

Pradhan J, Das BK, Sahu S. Evaluation of antibacterial and haemolytic activity of phytochemicals from freshwater microalgae, Euglena viridis (ehren). Int J Pharm Pharm Sci 2018;10:98-104.

Anand DC, Meena R, Patni V. In vitro callus induction and comparative GC-MS analysis of methanolic extracts of callus and leaf samples of Ampelocissus latifolia (roxb.) Planch. Int J Pharm Pharm Sci 2018;10:68-72.

Nazzaro F, Caliendo G, Arnesi G, Veronesi A, Sarzi P, Fratianni F. Comparative content of some bioactive compounds in two varieties of Capsicum annuum L. Sweet pepper and evaluation of their antimicrobial and mutagenic activities. J Food Biochem 2009;33:852-68.

Szabo M, Radu D, Gavrilas S, Chambre D, Iditoiu C. Antioxidant and antimicrobial properties of selected spice extracts. Int J Food Prop 2010;13:535-45.

Mukherjee PK, Sahu M, Suresh B. Indian herbal medicines. East Pharm 1998;490:21-3.

Vimala G, Shoba FG, Pandikumar P, Sukumar E. Pharmacological evaluation of ethanol extract of F. benghalensis seeds for antiulcer and antimicrobial efficacy. Indian J Nat Prod Resour 2017;8:329-34.

Nadkarni K. Indian Materia Medica. Vol. 1. Bombay: Popular Prakashan Publication; 1999. p. 543.

Balick M, Paul J, Cox A. Plants that heal; People and culture. Sci Ethnobotany 1996;73:25-61.

Shulz V, Rudolf H, Mark B. Medicinal plants, phytomedicines and phytotherapy. In: A physician’s Guide to Herbal Medicine. Vol. 4. New York: Springer; 2001. p. 1-39.

Aeri V, Singh D. HPTLC studies on various extracts of Acanthus ilicifolius Linn leaves and evaluation of antimicrobial and antioxidant activity. Der Pharm Lett 2013;5:261-73.

Arunkumar S, Valen P, Tina G, Deep A, Anitapriyadharshini K, Rekha D, et al. Preliminary phytochemical analysis, HPTLC finger printing and in vitro anticancer screening of extracts of aerial parts of Cardiospermum helicacabum. Int J Pharm Pharm Sci 2013;5:301-4.

Ivanova A, Delcheva I, Tsvetkova I, Kujumgiev A, Kostova I. GC-MS analysis and antimicrobial activity of acidic fractions obtained from Paeonia peregrine and Paeonia tenuifolia roots. Z Naturforsch 2002;57:624-8.

Vimala G, Shoba FG. Qualitative and quantitative determination of secondary metabolites and antioxidant potential of F. benghalensis Linn seed. Int J Pharm Pharm Sci 2015;7:118-24.

Bors W, Michel C. Chemistry of the antioxidant effect of polyphenols. Ann N Y Acad Sci 2002;1:57-69.

Pa R, Mathew L. Antimicrobial activity of leaf extracts of Justicia adhatoda in comparison with vasicine. Asian Pac J Trop Biomed 2012;2:l1556-60.

Soni A, Dahiya P. Screening of phytochemicals and antimicrobial potential of extracts of Vetiver zizanoides and Phragmites karka against clinical isolates. Int J App Pharm 2015;7:22-4.

Ram M, Abdin MZ, Khan MA, Prabhakar J. HPTLC fingerprint analysis: A quality control for authentication of herbal phytochemicals. Springer-Verlag Berl 2011;2:105-16.

Bodoprost J, Rosemeyer H. Analysis of phenacylester derivatives of fatty acids from human skin surface sebum by reversed phase HPTLC: Chromatographic mobility as a function of physicochemical properties. Int J Mole Sci 2007;8:1111-24.

Falodun A, Siraj R, Choudhary MI. GC-MS analysis of insecticidal leaf essential oil of Pyrenacantha staudtii Hutch and Dalz. Trop J Pharm Res 2009;8:139-43.

Published

07-01-2019

How to Cite

G, V., and G. Shoba F. “IN VITRO ANTIMICROBIAL ACTIVITY, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC SEED EXTRACT OF FICUS BENGHALENSIS LINN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 1, Jan. 2019, pp. 205-11, doi:10.22159/ajpcr.2019.v12i1.28818.

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