PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF PLECTRANTHUS BOURNEAE GAMBLE (LAMIACEAE)

  • R Thaniarasu Bharathidasan University
  • T Senthil Kumar Bharathidasan University
  • M. N Abubacker National College, Bharathidasan University
  • M. V Rao Bharathidasan University

Abstract

Objective: The aim was to investigate the antibacterial activity of Plectranthus bourneae Gamble (Lamiaceae) using different solvents.
Methods: Petroleum ether, chloroform, acetone, ethanol and aqueous leaves extracts at different concentration (25, 50, 75, 100 μg/ml) were
evaluated for antibacterial activity using disc diffusion method against some Gram-positive species namely Bacillus subtilis, Clostridium sporogenes,
Staphylococcus aureus, Streptococcus mutans, Streptococcus mitis and Gram-negative species Salmonella typhi, Klebsilla pneumoniae, Entrobactor
aerogenes, Citrobactor freundii, Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bacterial concentrations (MBC) were
determined by serial dilution method.
Results: The antibacterial activity results of ethanol extract (50 μg/ml) showed maximum zone of inhibition (26 mm) against S. aureus and MIC
value of 0.78 μg/ml and MBC of 1.56 μg/ml whereas aqueous extract was least effective on all the strains. The Gram-positive strains of S. aureus and
S. mutans were found to be the most sensitive and resistance on all the extracts. Except S. typhi, Gram-negative bacteria used were less inhibitory
effect to all the extracts.
Conclusion: The present study concluded that the ethanolic extracts of P. bourneae possess potential antibacterial activities against certain
microorganisms.

Keywords: Plectranthus bourneae, Antibacterial activity, Disc diffusion method, Lamiaceae.

Author Biographies

R Thaniarasu, Bharathidasan University
Department of Plant Science, Doctoral Research Student.
T Senthil Kumar, Bharathidasan University
Assistant Professor, Department of Industry University collabration.
M. N Abubacker, National College, Bharathidasan University
Associate professor and Head, Department of Biotechnology.
M. V Rao, Bharathidasan University
Honarary Professor, Department of Plant Science

References

1. Lukhoba C W, Simmonds MS, Paton AJ. Plectranthus: a review of
ethnobotanical uses. J Ethnopharmacol 2006;103(1):1-24.
2. Abdel-Mogib M, Albar HA, Batterjee SM. Chemistry of the genus
Plectranthus. Molecules 2002;7:271-301.
3. Morton JF. Country borage (Coleus aromaticus Lour.): A potent flavoring
and medicinal plant. J Herbs Spices Med Plants 1992;1(77):1- 2.
4. Abulfaith HA. Medicinal Plants in Southwestern Saudi Arabia.
Khamis: Al Thaghr Press: 1987. p. 162.
5. Gurgel AP, da Silva JG, Grangeiro AR, Oliveira DC, Lima CM, da
Silva AC, et al. In vivo study of the anti-inflammatory and antitumor
activities of leaves from Plectranthus amboinicus (Lour.) Spreng
(Lamiaceae). J Ethnopharmacol 2009;125:361-3.
6. Matu EN, van Staden J. Antibacterial and anti-inflammatory activities
of some plants used for medicinal purposes in Kenya. J Ethnopharmacol
2003;87(1):35-41.
7. Gaspar-Marques C, Simões MF, Duarte A, Rodríguez B. Labdane
and kaurane diterpenoids from Plectranthus fruticosus. J Nat Prod
2003;66(4):491-6.
8. Wellsow J, Grayer RJ, Veitch NC, Kokubun T, Lelli R, Kite GC,
et al. Insect-antifeedant and antibacterial activity of diterpenoids from
species of Plectranthus. Phytochemistry 2006;67(16):1818-25.
9. Runyoro DK, Matee MI, Ngassapa OD, Joseph CC, Mbwambo ZH.
Screening of Tanzanian medicinal plants for anti-Candida activity.
BMC Complement Altern Med 2006;6:11.
10. Murthy PS, Ramalakshmi K, Srinivas P. Fungitoxic activity of
Indian borage (Plectranthus ambonicus) volattiles, Food Chem
2009;114:1014-8.
11. Matthew KM. Precursory notes on the flora of the Palni (Pulney) Hills,
South India: II. Kew Bull 1993;48:757-65.
12. Matthew KM. Flora of Palni Hills. Suppl. III. Tiruchirapalli: Rapinat
Herbarium; 1998. p. 1117.
13. Harborne JB. Phytochemical Methods. A Guide to Modern Techniques
of Plant Analysis. London: Chapman and Hall; 1984.
14. Khandelwal KR. Practical Pharmacognosy Techniques and
Experiments. 17th ed. Pune: Nirali Prakashan; 2007.
15. Stokes EJ. Clinical Bacteriology. London: Edward Arnold Ltd.; 1975.
p. 208.
16. Sandberg F, Bruhn JG. Screening of plants for biologically active
substances. In: Soforowa EA, editor. African Medicinal Plants. Lagos:
University of Ife Press; 1979. p. 119.
17. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol
Rev 1999;12(4):564-82.
18. Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk
N, et al. Plants and human health in the twenty-first century. Trends
Biotechnol 2002;20(12):522-31.
19. Egwaikhide PA, Gimba CE. Analysis of the phytochemical and
anti- microbial actvity of Plectranthus glandulosis whole plant. Middle
East J Sci Res 2003;2(3-4):135-8.
20. Okwu DE. Evaluation of the chemical composition of indigenous
spices and flavouring agent. Glob J Pure Appl Sci 2001;7(3):455-9.
21. Sofowora A. Medicinal Plants and Traditional Medicine in Africa.
New York: John Wiley and Son Ltd.; 1993. p. 150-3.
22. Rijo P, Rodriguez B, Duarte A, Simoes MF. Antimicrobial properties of
Plectranthus ornatus extracts, 11-acetoxyhalima-5, 13-dien-15-oic acid
metabolite and its derivatives. J Nat Prod 2011;1:57-64.
23. Oyedemi SO, Afolayan AJ. Antibacterial and antioxidant activities of
hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pac J
Trop Med 2011;4(12):952-8.
24. Ullah MO, Haque M, Urmi KF, Zulfiker AH, Anita ES, Begum M, et al.
Anti-bacterial activity and brine shrimp lethality bioassay of methanolic
extracts of fourteen different edible vegetables from Bangladesh. Asian
Pac J Trop Biomed 2013;3(1):1-7.
25. Bhattacharya S, Zaman MK, Haldar PK. Antibacterial activity of stem
bark and root of Indian Zanthoxylum nitidum. Asian J Pharm Clin Res
2009;2(1):30-4.
26. Venkatesh Krishna V, Girish Kumar K, Pradeepa K, Santhosh
Kumar SR. Antibacterial activity of ethanol extract of Musa paradisiaca
CV. Puttable and Musa acuminate CV. Grand naine. Asian J Pharm Clin
Res 2013;6(2):169-72.
Statistics
316 Views | 668 Downloads
How to Cite
Thaniarasu, R., T. Senthil Kumar, M. N. Abubacker, and M. V. Rao. “PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF PLECTRANTHUS BOURNEAE GAMBLE (LAMIACEAE)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 1, Jan. 2015, pp. 79-82, https://innovareacademics.in/journals/index.php/ajpcr/article/view/2899.
Section
Original Article(s)