• Burhan Ma'arif Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, East Java, Indonesia.
  • Agnis Pondinekaria Aditama Depatment of Pharmacy, Pharmacy Academy of Jember, Jember, East Java, Indonesia.


Objectives: This study was conducted to analyze the anti-osteoporosis effect of 96% ethanol extract from Chrysophyllum cainito L. leaves, which suspected to contain phytoestrogens, in increasing the osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice.

Materials and Methods: The 96% ethanol extract of C. cainito leaves was given to male mice with dose of 2, 4, 8, and 16 mg/20 g BB mice/day after being induced orally with dexamethasone dose of 0.0029 mg/20 g BB mice/day. The positive control used was 0.026 ml/20 g BB mice/day alendronate. After 4 weeks, the increasing of osteoblast cell number in trabecular vertebrae bone of male mice was observed using an optical microscope with 100× zoom in after histomorphometry and hematoxylin-eosin staining methods.

Results: The result showed the significant increasing of osteoblast number in trabecular vertebrae bone of male mice in all groups after being given treatment using 96% ethanol extract of C. cainito, with an effective dose (ED50) value of 9.5 mg/20 g BB mice/day. This increase is suspected due to the phytoestrogens content, which can also act as phytotestosterone in 96% ethanol extract of C. cainito.

Conclusion: This study concluded that 96% ethanol extract of C. cainito has an activity in increasing osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice, with an ED50 value of 9.5 mg/20 g BB mice/day.

Keywords: Chrysophyllum cainito L., Phytoestrogens, Osteoporosis.


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How to Cite
Ma’arif, B., and A. P. Aditama. “ACTIVITY OF 96% ETHANOL EXTRACT OF CHRYSOPHYLLUM CAINITO L. IN INCREASING VERTEBRAE TRABECULAR OSTEOBLAST CELL NUMBER IN MALE MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 286-8, doi:10.22159/ajpcr.2019.v12i1.28994.
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