EFFECT OF IMIDACLOPRID INSECTICIDE RESIDUE ON BIOCHEMICAL PARAMETERS IN POTATOES AND ITS ESTIMATION BY HPLC
Pesticides are known to interfere with the biochemical processes of plants, lowering their food quality. The effects of imidacloprid insecticide (a very commonly used pesticide on vegetables) on some biochemical quality control parameters and some enzyme systems (CAT, PPO and POD) were studied. Estimation of imidacloprid residues in potatoes was done by HPLC. It was observed that insecticide treated potatoes have considerable amount of imidacloprid 0.35 mg/kg at the time of harvesting. Washing potatoes with tap water and boiling for 20min. decreased residue up to 33% and 80% respectively. It was also found that the imidacloprid insecticide treatment decreased the reducing sugar, total phenols, ortho-dihydroxy phenols and ascorbic acid contents of potatoes but increased the total protein content and enzyme (catalase, peroxidase and polyphenol oxidase) activity.
Keywords: Imidacloprid, potato, HPLC, biochemical parameters, enzyme activity
2. Lee Fook Choy LH and Seeneevassen S. Monitoring insecticide residues in vegetables and fruits at the market level. AMAS, Food and Agricultural Research Council, Reduit, Mauritius, 1998; pp. 95.
3. Misra SG and Mani D. Adverse effects of Pesticides. In: Agricultural pollution II. 1994; (Eds.):S.G. Misra and D. Mani Ashish Publisher, New Delhi.
4. Chairman K, Singh AJAR and Padmalatha C. Effect of pesticides on intestinal micro flora in the fish, Mystus vittatus. Asian Journal of Pharmaceutical and Clinical Research 2011; 4 (4):156-158.
5. Kadenezki L, Arpad Z, Gardi I, Ambrus A, Gyorfi L and Reese G. Column extraction of residues of several pesticides from fruits and vegetables; a simple multiresidue analysis method. Journal of AOAC International 1992; 75:53-61.
6. Miller GL. Use of dinitrosalicylic acid (DNS) reagent for determination of reducing sugar. Analytical Chemistry 1959; 31 (3):426-428.
7. Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry 1976; pp 248-254.
8. Singleton VL and Rossi JA. Colorimetry of total phenolics with phosphomolybdic and phosphotungtic acid reagents, American Journal of Enology and Viticulture 1965; 16:144-158.
9. Arnow LE. Colorimetric determination of the components of 2, 4-dihydroxyphenyl alanine-tyrosine mixtures, Journal of Biology and Chemistry 1937; 118:531-53.
10. Niewiadomski H. Rape seed Chemistry and Technology. Elsevier Science Publisher, Amsterdam, Netherland, 1990; pp. 66-62.
11. Beers RF and Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, Journal of Biology Chemistry 1952; 195:133-140.
12. Tatiana Z, Yamashita K and Matsumoto H. Iron deficiency induced changes in ascorbate content and enzyme activities related to ascorbate metabolism in cucumber root Plant Cell Physiology 1999; 40:273-280.
13. Jockusch H. The role of host genes, temperature and polyphenol oxidase in the necrotization of TMV infected tobacco tissue. Phytopathologische Zeischrift 1966; 55:185-192
14. Bartholomew ET, Stewart WS and Carman GE. Some physiological effects of insecticides on citrus fruits and leaves, The Botanical Gazette 1951; 112(4):501-511.
15. Habiba RA, Ali HM and Ismail SMM. Biochemical effects of Profenofos residues in potatoes. Journal of Agriculture Food Chemistry 1992; 40:1852-1855.
16. Ismail SMM, Ali HM and Habiba R. GC-ECD and GC-MS analysis of profenofos residues and the biochemical effects in tomatoes and tomato products, Journal of Agriculture Food Chemistry 1993; 41:610-615.
17. Hesam F, Balali GR and Tehrani RT. Evaluation of antioxidant activity of three common potato (Solanum tuberosum) cultivars in Iran. Avicenna Journal of Phytomedicine 2012; 2(2):79-85.
18. Radwan MA, Shiboob MH, Abu-Elamayem MM and Abdel-Aal A. Residues of primiphos-methyl and profenofos on green pepper and eggplant fruits and their effects on some quality properties Emirates Journal of Agriculture Science 2004; 16(1):32-42.
19. Bidwell RG, Barr RA and Steward FC. Protein synthesis and turnover in cultured tissue: Sources of carbon for synthesis and the fate of the protein breakdown products, Nature 1964; 203:367-373.
20. Rouchaud J, Moons C, Detroux L, Haquenne W, Seutin E. and Nys L. Quality of potatoes treated with selected insecticides and potato-halum killers. Journal of Horticulture Science 1986; 61(2):239-242.
21. Galante YM and Formantici C. Enzyme Applications in Detergency and in Manufacturing Industries. Current Organic Chemistry 2003; 7(13):1399.
22. Burnette FS. Peroxides and its relationship to food flavour and quality: a review. J. Food Science 1977; 42:1-5.
23. Shinkle JR and Jones RL. Inhibition of stem elongation in cucumis seedling by blue light requires calcium. Plant Physiology 1988; 86:960-966.
24. Pande S and Khetmalas M. Quantitative Estimation Of Biochemical Content Of Various Extracts Of Stevia Rebaudiana Leaves. Asian Journal of Pharmaceutical and Clinical Research 2012; 5 (1):115-117.
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