IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF ISOLATED COMPOUNDS ON ROOTS OF CLERODENDRUM PHLOMIDIS LINN
Objective: The main aim of the study was to screen the isolated compounds of Clerodendrum phlomidis roots for its in vitro antioxidant and anticancer
activity and its efficacy against HeLa cell lines.
Methods: Pet ether, chloroform, ethyl acetate and ethanol extracts was prepared and assayed for the presence of phytochemicals. Three
compounds were isolated from ethanol extract of C. phlomidis by column chromatography such as ET1 (phenyl acetic acid), ET2 (ethyl-2-hydroxy-
4-methylbenzoate), ET3 (3,6,7-trihydroxy-2-(3-methoxyphenyl)-4H-chromen-4-one) characterized by IR (KBr), 1H-nuclear magnetic resonance
(NMR), 13C-NMR and Gas chromatographyâ€“mass spectrometry. The above isolated compounds were subjected to in vitro antioxidant activity against
2,2-diphenylpicrylhydrazyl (DPPH) radical, superoxide radical scavenging assay and iron chelating activity. The effect of isolated compounds on HeLa
cancer cell line was also evaluated by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide colorimetric assay.
Results: ET2 has good in vitro antioxidant activity against DPPH radical, super oxide radical and iron chelating activity with an inhibitory concentration
50% value of 360 Î¼g/ml, 150 Î¼g/ml and 130 Î¼g/ml respectively. ET1 showed significant cytotoxic activity than the other two compounds on HeLa
cells with a percentage cell growth of 21.9% and a growth inhibition of 50% value of 180 Î¼g, respectively.
Conclusion: On the basis of obtained results, ET1 and ET2 isolated from the ethanolic extract of C. phlomidis represent a new group of cytotoxic
against HeLa cell line and antioxidant agents.
Keywords: Column chromatography, Clerodendrum phlomidis, In vitro antioxidant activity, 2,2-diphenylpicrylhydrazyl, Cytotoxicity,
3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide assay.
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