• SELVARANI M Department of Zoology, Sree Sevugan Annamalai College, Devakottai, Tamil Nadu, India.
  • JEYASIMGA V Department of Biotechnology, V. V. Vanniaperumal College for Women (Autonomous), Virudhunagar, Tamil Nadu, India.



Justicia adhatoda, Phytochemical screening,, Bactericidal potency, Activity index, Fold area increase


Objective: Increasing prevalence of bacterial resistance has made an important public health issue in the modern world. Nowadays, many research efforts have been directed toward the development of drugs from medicinal plants for the treatment of diseases caused by microorganisms. Hence, the present attempt has been made to evaluate the bactericidal potency of traditional medicinal plant, Justicia adhatoda against selected clinical pathogens.

Methods: Leaf extracts of J. adhatoda were subjected to standard phytochemical analyses by qualitative color tests to confirm the presence of active Phytoconstituents. Bactericidal potency of the leaf extracts of J. adhatoda was assessed by growth inhibition zone against Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Sensitivity of the tested bacterial strains was also checked with standard reference antibiotic vancomycin.

Results: Qualitative phytochemical analyses revealed the presence of alkaloids, terpenoids, flavonoids, tannins, steroids, anthraquinones, saponins, resins, glycosides, and phenols. Fourier-transform infrared spectrum confirmed the presence of phytochemicals belong to various functional groups such as alkenes, alkanes, aliphatic and aromatic amines, aldehydes, esters, alcohols, and phenols. Results on bactericidal efficacy demonstrated that solvents used in the present experiment showed higher activity in the order ethanol >ethyl acetate >chloroform >diethyl ether >methanol >acetone >water. Leaf extracts of J. adhatoda showed the higher activity for different clinical pathogens in the order of P. aeruginosa >B. cereus >E. coli >K. pneumoniae >S. aureus. Among the tested bacterial pathogens, B. cereus exhibited maximum susceptibility (18.33±0.94 mm) toward ethyl acetate extract. The maximum activity index (2.67±0.42) and fold area increase (6.31±2.24) were also observed against B. cereus for ethyl acetate extract.

Conclusion: The present study revealed that J. adhatoda has broad spectrum of antibacterial activity and a potential source of antibacterial agents that could be useful for the control of infectious diseases.


Download data is not yet available.


Hidayathulla S, Chandra KK, Chandrashekar KR. Phytochemical evaluation and antibacterial activity of Pterospermum diversifolium Blume. Int J Pharm Pharm Sci 2011;3:165-7.

Rashmi P, Mathew L. Antimicrobial activity of leaf extracts of Justicia adhatoda L. in comparision with vasicine. Asian Pac J Trop Biomed 2012;2:1556-60.

Chopra RN, Nayer SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi, India: Council of Scientific and Industrial Research; 1956.

Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 2001;74:113-23.

Mohamed HS, Abdelgadir WS, Almagboul AZ. In vitro antimicrobial activity of anise seed (Pimpinella anisum L.). Int J Adv Res 2015;3:359-67.

Hamoud R, Sporer F, Reichling J, Wink M. Antimicrobial activity of a traditionally used complex essential oil distillate (Olbas Tropfen) in comparison to its individual essential oil ingredients. Phytomedicine 2012;19:969-76.

Afolabi CA, Ibukun EO, Emmanuel A, Obuotor EM, Farombi EO. Phytoconstituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essays 2007;2:163-6.

Daniel VN, Daniang IE, Nimyel ND. Phytochemical analysis and mineral elements composition of Ocimum basilicum obtained in JOS metropolis, Plateau State, Nigeria. Int J Eng Technol. 2011;11:161-5.

Dhankhar S, Kaur R, Ruhil S, Balhara M, Dhankhar S, Chhillar AK. A review on Justicia adhatoda: A potential source of natural medicine.Afr J Plant Sci 2011;5:620-7.

Srivastava S, Verma RK, Subhash MM, Singh C, Kumar S. HPLC determination of vasicine and vasicinone in Adhatoda vasica with photo diode array detection. J Liq Chromatogr Relat Technol 2001;24:153-9.

Kumar A, Ram J, Samarth RM, Kumar M. Modulatory influence of Adhatoda vasica Nees leaf extract against gamma irradiation in Swiss albino mice. Phytomedicine 2005;12:285-93.

Ilango K, Chitra V, Kanimozhi P, Balaji G. Antidiabetic, antioxidant and antibacterial activities of leaf extracts of Adhatoda zeylanica. Medic (Acanthaceae). J Pharm Sci Res 2009;2:67-73.

Trease GE, Evans WC. Pharmacognosy. Vol. 14. London: WB Scandars Company Ltd.; 1989. p. 269-300.

Sahu M, Vermaand D, Harris K. Phytochemical analysis of the leaf, stem and seed extracts of Cajanus cajan L. (Dicotyledoneae: Fabaceae). World J Pharm Pharm Sci 2014;3:694-733.

Singariya P, Kumar P, Mourya KK. Antimicrobial activity of fruit coat (calyx) of Withania somnifera against some multi drug resistant microbes. Int J Biol Pharm Res 2012;3:252-8.

Selvarani M, Prema P. Evaluation of antibacterial efficacy of chemically synthesized copper and zerovalent iron nanoparticles. Asian J Pharm Clin Res 2013;6:223-7.

Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effects of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine 2007;3:168-71.

Sankar SG, Thangapandiyan S, Alisha AS, Kumar PS. Phytochemical screening and potential bactericidal efficacy of Annona squamosa leaf extracts. World J Pharm Life Sci 2018;4:144-9.

Waterman PG, Mole S. Methodology in ecology. In: Analysis of Phenolic Plant Metabolites. London: Blackwell Science Publications; 1994. p. 66-103.

Vairavan S, Thangapandiyan S, Alisha AS. Larvicidal efficacy of Catharanthus roseus leaf extracts against filarial vector Culex quinquefasciatus (Diptera: Culicidae). Int J Pharm Sci Rev Res 2018;51:19-25.

Jayapriya G, Shoba FG. Phytochemical analysis, antimicrobial efficacy and determination of bioactive components from leaves of Justicia adhatoda (Linn). Asian J Plant Sci Res 2015;5:43-51.

Ahmad S, Garg M, Ali M, Singh M, Athar MD, Ansari SH. A phytopharmacological overview on Adhatoda zeylanica Medic. syn. A. vasica (Linn.) Nees. Nat Prod Rad 2009;8:549-54.

Tilak R, Punit K, Radha R, Kumar DK. Screening of some medicinal plants for their antimicrobial activities. Int J Pharm Pharm Sci 2016;8:202-6.

Kanyanga CR, Munduku KC, Ehata TM, Lumpu NS, Maya MB, Manienga K, et al. Antibacterial and antifungal screening of extracts from six medicinal plants collected in Kinshasa-Democratic Republic of Congo against clinical isolate pathogens. J Pharmacogn Phytother 2014;6:24-32.

Hassan MH, Ismail MA, Moharram AM, Shoreit AA. Phytochemical and antimicrobial of latex serum of Calotropis procera and its silver nanoparticles against some reference pathogenic strains. J Ecol Health Environ 2017;5:65-75.

Baydar H, Sagdic O, Ozkan G, Karadogan T. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food control 2004;15:169-72.

Upadhyay A, Mooyottu S, Yin H, Nair S, Bhattaram V, Venkitanarayanan K. Inhibiting microbial toxins using plant-derived compounds and plant extracts. Medicines (Basel) 2015;2:186-211.



How to Cite

M, S., and J. V. “PHYTOCHEMICAL SCREENING AND BACTERICIDAL POTENCY OF JUSTICIA ADHATODA AGAINST CLINICAL PATHOGENS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 10, Oct. 2020, pp. 72-76, doi:10.22159/ajpcr.2020.v13i10.29851.



Original Article(s)