VASOPROTECTIVE EFFECT OF PHYSALIS ANGULATA L. LEAF WATER EXTRACT ON KIDNEY OF NΩ-NITRO-L-ARGININE METHYL ESTER-INDUCED ENDOTHELIAL DYSFUNCTION RAT MODEL

  • Zahrah Febianti Department of Biomedical Sciences, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
  • Nur Permatasari Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
  • Setyawati Soeharto Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.

Abstract

Objective: This study was to investigate the effect of Physalis angulata L. leaf water extract on vascular rarefaction, oxidative stress, and inflammation on the kidney of Nω-nitro-L-arginine methyl ester (L-NAME)-induced male Wistar rats.

Methods: A total of 25 male Wistar rats were divided into five equal groups (normal control: 40 mg/kg/day of normal saline; L-NAME group; and treatments I, II, and III: L-NAME plus P. angulata L. leaf water extract doses 500 mg/kg/day, 1500 mg/kg/day, and 2500 mg/kg/day, respectively). Endothelial dysfunction was induced by 40 mg/kg/day L-NAME intraperitoneally. The treatment lasts for 15 days. Vascular rarefaction was indicated by the decrease in vascular density, which considered as vascular number per high-power field in hematoxylin-eosin staining preparation. Kidney oxidative stress test was performed by measuring malondialdehyde (MDA) level with thiobarbituric acid reactive substances assay. The inflammatory marker was the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) expression which examined using an immunohistochemical method with an antibody against p65.

Results: At the dose of 500 mg/kg/day and 1500 mg/kg/day, P. angulata leaf water extract supplementation increased the vascular density, decreased the MDA level, and decreased the NF-κB expression compared to the L-NAME group.

Conclusion: The administration of P. angulata L. leaf water extract in particular concentration has a vasoprotective effect by preventing kidney vascular rarefaction, oxidative stress, and inflammation on L-NAME-induced male Wistar rat.

Keywords: Endothelial dysfunction, Kidney, Malondialdehyde, Nuclear factor kappa-light-chain-enhancer of activated B cells, Nω-nitro-L-arginine methyl ester, Physalis angulata leaf, Vasoprotective.

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Febianti, Z., N. Permatasari, and S. Soeharto. “VASOPROTECTIVE EFFECT OF PHYSALIS ANGULATA L. LEAF WATER EXTRACT ON KIDNEY OF NΩ-NITRO-L-ARGININE METHYL ESTER-INDUCED ENDOTHELIAL DYSFUNCTION RAT MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 432-7, doi:10.22159/ajpcr.2019.v12i1.29857.
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