• Yunita Sari Department of Nursing, Jenderal Soedirman University, Indonesia.
  • Iwan Purnawan Department of Nursing, Jenderal Soedirman University, Indonesia.
  • Eman Sutrisna Department of Pharmacology, Jenderal Soedirman University, Indonesia.
  • Dhadhang Wahyu Kurniawan Department of Pharmaceutics, Jenderal Soedirman University, Indonesia.
  • Nasruddin Nasruddin Department of Medical Laboratory Science, Universitas Muhammadiyah Semarang, Indonesia.


Objective: The objective of this research was to evaluate the effect of different concentrations of Aloe vera (AV) on inflammation of diabetic ulcers in a rat model.

Methods: The induction of diabetes was conducted by injection of alloxan monohydrate. This study involved four groups, including 0%, 10%, 30%, and inner leaf AV (ILAV) gel groups. Necrotic tissue, wound size, inflammation, and reepithelialization were evaluated. Wound tissue was collected on days 4 and 8. Histological analysis was performed by hematoxylin and eosin staining. Differences in the intensity of inflammation were analyzed using Kruskal–Wallis test followed by Mann–Whitney U-test.

Results: On day 13, wounds in gels containing AV were almost completely healed, whereas wounds in the 0% gel group (control) were still covered with necrotic tissue. On day 3, the intensity of inflammation in the 30% and ILAV groups was significantly less when compared to the 10% and control groups. On day 8, inflammation was less significant in the 10%, 30%, and ILAV groups when compared with the control group. Moreover, the intensity of inflammation in the 30% and ILAV groups was significantly less compared to the 10% group. In addition, reepithelialization was more advanced in the 10% and 30% ILAV groups compared with the control group, and reepithelialization was most advanced in the 30% and ILAV groups.

Conclusion: Our study indicated that gels containing AV reduced inflammation and improved reepithelialization of diabetic ulcers in a rat model. Therefore, it is highly recommended for clinicians to use AV gels for topical treatment of diabetic ulcers.

Keywords: Aloe vera, Diabetic ulcer, Inflammation, Rat, Reepithelialization.


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How to Cite
Sari, Y., I. Purnawan, E. Sutrisna, D. W. Kurniawan, and N. Nasruddin. “EVALUATION OF THE EFFECT OF DIFFERENT CONCENTRATIONS OF ALOE VERA ON INFLAMMATION AND REEPITHELIALIZATION IN DIABETIC ULCERS IN A RAT MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 15, Oct. 2018, pp. 32-35, doi:10.22159/ajpcr.2018.v11s3.30024.
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