ANTIOXIDANT ACTIVITY OF ETHYL ACETATE FRACTION OF MACARANGA TRILOBA LEAVES FROM CENTRAL KALIMANTAN


Syahrida Dian Ardhany, Dewi Sari Mulia, Pienyani Rosawanti

Abstract


Objective: Macaranga triloba was used by the local for diabetes, antidiabetes have strong relation with antioxidant activity, antioxidants have been shown to prevent the destruction of β-cells by inhibiting the preoxidation chain reaction, plants containing natural antioxidant (like flavonoid and alkaloid) can protect β cell function. So the objective of this study is to investigate antioxidant activity of ethyl acetate fraction of Macaranga triloba leaves and did preliminary phytochemical screening (Alkaloid and Flavonoid).

Methods: Macaranga triloba leaves (Mahang damar) were collected from the local areas of Bukit Rawi Central Kalimantan. Macaranga triloba leaves were extracted using maceration methods, followed by fractionation using liquid-liquid extraction methods starting from n-hexane, ethyl acetate, methanol but in this research used ethyl acetate fraction of Macaraga triloba and preliminary phytochemical screening for alkaloid used dragendorff and flavonoid used Pb Acetate. Antioxidant activity test was carried out using 1,1-diphenyl-2-pikrilhidrazil (DPPH) with quercetin as a reference.

Results: It was found that ethyl acetate fraction of Macaranga triloba leaves containing positive flavonoid that has been shown by dragendorff methode (brick red sedimen) and positive alkaloid by Pb acetate. The inhibition concentration 50 (IC50) value of ethyl acetate fraction of Macaranga triloba leaves is 56.93 ppm

Conclusion: Macaranga triloba leaves by preliminary screening of phytochemical containing alkaloid and flavonoid. Antioxidant activity of ethyl acetate fraction with IC50 = 56.93 ppm is weaker than quercetin (IC50 = 4.46 ppm), but still in range value of strong antioxidant activity (50-100 ppm)


Keywords


Antioxidant, Central Kalimantan, Macaranga triloba leaves, Quercetin, 1,1-diphenyl-2-pikrilhidrazil (DPPH).

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References


Mustarichie R, Sandra M, Wiwiek I, Ade Z. Antioxidant activity of tauco extract and its fractions. Asian J Pharm Clin Res 2017;2017:57-61.

Bharti R, Gunja A, Ganapathy PS, Shruthi SD. A review on medicinal plants having antioxidant potential. J Pharm Res 2012;5:4278-87.

Zhang YJ, Gan RY, Sha Li, Zhou Y, Li AN, Xu DP, et al. Review antioxidant phytochemicals for the prevention and treatment of chronic diseases. MDPI Molecules J 2015;20:21138-56.

Sas K, Robotka H, Toldi J, Vecsei L, Mitochondrial, metabolic disturbances, oxidative stress, and kynurenine system, with focus on neurodegenerative disorders. J Neural Sci 2007;257:221-39.

Aminah NS, Alfinda NK, Mulyadi T. Antioxidant activity of flavonoid compounds from the leaves of Macaranga gigantean. J Chem Pharm Res 2014;6:688-92.

Lim TY, Lim YY, Yule CM. Bioactivity of leaves of Macaranga species in tropical peta swamp and non peta swamp environments. J Trop For Sci 2014;26:134-41.

Puteri MD, Kawabata J. Novel α glucosidase inhibitors from Macaranga tanarius Leaves. Food Chem 2010;123:384-9.

Shekar TC, Anju G. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. Leaves. Am J Ethnomed 2014;1:244-9.

Nithya TG, Jayanthi J, Ragunathan MG. Antioxidant activity, total phenol, flavonoid, alkaloid, tannin and saponin contents of leaf extracts of Salvinia molesta. Asian J Pharm Clin Res 2016;9:200-3.

Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Sci World J 2013;2013:162750.

Jebbita SR, Jeyanth AS. Antioxidant activity, total phenol, flavonoid and anthocyanin contents of jamun (Syzygium cumini) pulp powder. Asian J Pharm Clin Res 2016;9:361-3.

Goveas SW, Asha A. Evaluation of antimicrobial and antioxidant activity of stem and leaf extracts of Coscinium fenestratum. Asian J Pharm Clin Res 2013;6:218-21.

Sowndhararajan K, Sun CK. Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight and Arn. Saudi J Bio Sci 2013;20:319-25.

Tiong SH, Chung YL, Hazrina H, Aditya A, Mohammadjavad P, Won FW, et al. Antidiabetic and antioxidant properties of alkaloids from Catharantus roseus (L.) G.Don. Molecules J 2013;18:9770-84.

Usmani SA. Screening for antioxidant and free radical scavenging potential of extracts of leaves and flowers of Calotropis gigantea. Asian J Pharm Clin Res 2013;6:97-100.

Molineux P. The use of the stable free radical diphenyl picrylhydrazil (DPPH) for estimating antioxidant activity. Songklankarin J Sci Technol 2004;26:211-9.




About this article

Title

ANTIOXIDANT ACTIVITY OF ETHYL ACETATE FRACTION OF MACARANGA TRILOBA LEAVES FROM CENTRAL KALIMANTAN

Keywords

Antioxidant, Central Kalimantan, Macaranga triloba leaves, Quercetin, 1,1-diphenyl-2-pikrilhidrazil (DPPH).

DOI

10.22159/ajpcr.2018.v11s3.30026

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 40-42

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Syahrida Dian Ardhany
Department of Health Science, Muhammadiyah University of Palangkaraya, Central Kalimantan, Indonesia.
Indonesia

Dewi Sari Mulia
Department of Health Science, Muhammadiyah University of Palangkaraya, Central Kalimantan, Indonesia.
Indonesia

Pienyani Rosawanti
Department of Agriculture and Forestry, Muhammadiyah University of Palangkaraya, Central Kalimantan, Indonesia.
Indonesia


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