NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION

  • Mashuri Mashuri Department of Radiology, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
  • Achmad Zaini Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
  • Diana Rahmanisa Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
  • Muhammad Rifqi Alfiannoor Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
  • Muhammad Rosyhan Sanjaya Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
  • Eko Suhartono Department of Medical Chemistry/Biochemistry, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.

Abstract

Objective: The present study was undertaken to investigate the photohemolysis reaction through photosensitization reaction by ceftazidime as a photosensitizer in human red blood cell (RBC).

Methods: In this present study, human erythrocytes have used a sample. The sample then divided into six groups consisting of Group 1 (T1) served a negative control which consists of erythrocytes and buffers phosphate with pH 6.8; Group 2 (T2) served as a positive control which consists erythrocytes and buffers phosphate with pH 6.8 and exposed to UV-light; and Group 3, 4, 5, and 6 (T3, T4, T5, and T6) served as an experimental group which consists of erythrocytes, buffer phosphate with pH 6.8, ceftazidime with concentration 10%, 20%, 30%, and 40%, respectively, and also exposed to UV-light. UV-light exposure was done in 2 h. After the treatment period, the level of hydrogen peroxide (H2O2), conjugated diene (CD), advanced oxidation protein products (AOPPs), and percentage of RBC hemolysis (RBCH) were measured.

Results: The results of this present studies showed that ceftazidime significantly increases the levels of H2O2, CD, AOPPs, and percentage of RBCH during the UV radiation.

Conclusion: The present study demonstrated that ceftazidime acts as a photosensitizer and induced the photohemolysis reaction in human RBC. Furthermore, the hemolysis of RBC seems through the protein damage than lipid damage.

Keywords: Ceftazidime, Photohemolysis, Photosensitization, Photosensitizer.

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Mashuri, M., A. Zaini, D. Rahmanisa, M. R. Alfiannoor, M. R. Sanjaya, and E. Suhartono. “NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 15, Oct. 2018, pp. 47-50, doi:10.22159/ajpcr.2018.v11s3.30029.
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