NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION


Mashuri Mashuri, Achmad Zaini, Diana Rahmanisa, Muhammad Rifqi Alfiannoor, Muhammad Rosyhan Sanjaya, Eko Suhartono

Abstract


Objective: The present study was undertaken to investigate the photohemolysis reaction through photosensitization reaction by ceftazidime as a photosensitizer in human red blood cell (RBC).

Methods: In this present study, human erythrocytes have used a sample. The sample then divided into six groups consisting of Group 1 (T1) served a negative control which consists of erythrocytes and buffers phosphate with pH 6.8; Group 2 (T2) served as a positive control which consists erythrocytes and buffers phosphate with pH 6.8 and exposed to UV-light; and Group 3, 4, 5, and 6 (T3, T4, T5, and T6) served as an experimental group which consists of erythrocytes, buffer phosphate with pH 6.8, ceftazidime with concentration 10%, 20%, 30%, and 40%, respectively, and also exposed to UV-light. UV-light exposure was done in 2 h. After the treatment period, the level of hydrogen peroxide (H2O2), conjugated diene (CD), advanced oxidation protein products (AOPPs), and percentage of RBC hemolysis (RBCH) were measured.

Results: The results of this present studies showed that ceftazidime significantly increases the levels of H2O2, CD, AOPPs, and percentage of RBCH during the UV radiation.

Conclusion: The present study demonstrated that ceftazidime acts as a photosensitizer and induced the photohemolysis reaction in human RBC. Furthermore, the hemolysis of RBC seems through the protein damage than lipid damage.


Keywords


Ceftazidime, Photohemolysis, Photosensitization, Photosensitizer.

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About this article

Title

NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION

Keywords

Ceftazidime, Photohemolysis, Photosensitization, Photosensitizer.

DOI

10.22159/ajpcr.2018.v11s3.30029

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 47-50

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Mashuri Mashuri
Department of Radiology, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia

Achmad Zaini
Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia

Diana Rahmanisa
Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia

Muhammad Rifqi Alfiannoor
Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia

Muhammad Rosyhan Sanjaya
Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia

Eko Suhartono
Department of Medical Chemistry/Biochemistry, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Indonesia


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