PUBLIC HEALTH HAZARDS ASSOCIATED WITH NATURALLY OCCURRING TOXINS IN LEGUME SEEDS - RAPID DETECTION AND CHARACTERIZATION OF A LECTIN FROM A KOREAN CULTIVATED KIDNEY BEAN (PHASEOLUS VULGARIS L.)


Nader Nciri, Taesub Shin, Namjun Cho

Abstract


Objective: The delicacy of kidney beans is highly appreciable but, at the same time, their toxicity has raised an alarming concern. In this regard, more public awareness is needed about bean poisoning.

Methods: This work aimed at promptly investigating the chemical and biological properties of a lectin derived from a Korean cultivar of the common bean (Phaseolus vulgaris L.).

Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of two major polypeptide bands around 31.00 kDa. Hemagglutination assay and Ouchterlony double immunodiffusion technique confirmed the presence of lectin in dry seeds.

Conclusion: It was evident that the Korean bean cultivar contains biologically active lectins, which may pose a risk to the consumer if the beans are eaten in raw or undercooked state.


Keywords


Common bean (Phaseolus vulgaris L.), Hemagglutination assay, Korean cultivar, Lectin, Ouchterlony double immunodiffusion, Phytohemagglutinin, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, White kidney bean (Phaseolus vulgaris L.).

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About this article

Title

PUBLIC HEALTH HAZARDS ASSOCIATED WITH NATURALLY OCCURRING TOXINS IN LEGUME SEEDS - RAPID DETECTION AND CHARACTERIZATION OF A LECTIN FROM A KOREAN CULTIVATED KIDNEY BEAN (PHASEOLUS VULGARIS L.)

Keywords

Common bean (Phaseolus vulgaris L.), Hemagglutination assay, Korean cultivar, Lectin, Ouchterlony double immunodiffusion, Phytohemagglutinin, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, White kidney bean (Phaseolus vulgaris L.).

DOI

10.22159/ajpcr.2018.v11s3.30037

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 76-79

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Nader Nciri
School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.
Korea, Republic of

Taesub Shin
School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.
Korea, Republic of

Namjun Cho
School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.
Korea, Republic of


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