THE EFFECTIVENESS OF SNAIL MUCUS (ACHATINA FULICA) AND CHITOSAN TOWARD LIMFOSIT PROLIFERATION IN VITRO


Agnes Sri Harti, Atiek Murharyati, Dwi Sulisetyawati S, Meri Oktariani

Abstract


Objective: The objective of this research was to assess the effectiveness of mucous and chitin slime cream against lymphocyte proliferation in vitro.

Methods: The research methods include snail mucus isolation, snail mucus cream material preparation, and lymphocyte cell proliferation test of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method.

Results: Lymphocyte cell proliferation test of MTT reduction method showed that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream. The 5% snail mucus cream 5% provides a higher proliferative activity than the 100% snail mucus. Positive control using Con A solution. Negative control was treatment without addition of test solution. The differences in lymphocyte proliferation activity were due to the presence of active compounds in chitosan, snail mucus, and snail slime cream that potentially increase lymphocyte proliferation. Significant differences in lymphocyte cell proliferation as Group K1, K2, K3, K4, K5, and K6.

Conclusion: The effectiveness of snail and chitosan slime cream toward lymphocyte proliferation by in vitro that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream.


Keywords


Anti-inflammatory, In vitro, Lymphocyte, Proliferation, Snail mucus, Wound healing, In vitro.

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About this article

Title

THE EFFECTIVENESS OF SNAIL MUCUS (ACHATINA FULICA) AND CHITOSAN TOWARD LIMFOSIT PROLIFERATION IN VITRO

Keywords

Anti-inflammatory, In vitro, Lymphocyte, Proliferation, Snail mucus, Wound healing, In vitro.

DOI

10.22159/ajpcr.2018.v11s3.30041

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 85-88

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Agnes Sri Harti
Departement of Nursing, Kusuma Husada Surakarta School of Health Science, Surakarta, Indonesia.
Indonesia

Atiek Murharyati
Departement of Nursing, Kusuma Husada Surakarta School of Health Science, Surakarta, Indonesia.
Indonesia

Dwi Sulisetyawati S
Departement of Nursing, Kusuma Husada Surakarta School of Health Science, Surakarta, Indonesia.
Indonesia

Meri Oktariani
Departement of Nursing, Kusuma Husada Surakarta School of Health Science, Surakarta, Indonesia.
Indonesia


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