PHYTOCHEMICAL, MICROBIOLOGICAL, AND TECHNOLOGICAL STUDIES IN THE FIELD OF OBTAINING A HYDROALCOHOLIC EXTRACT WITH ANTIMICROBIAL ACTIVITY FROM THE LIQUORICE ROOT
Objective: The objective of this work was to carry out studies in the field of development of a hydroalcoholic extract from the liquorice root that has a high level of antimicrobial activity.
Methods: For the antibacterial study of extracts, we have used the agar well diffusion method. In our research, we have utilized six test-strain microorganisms: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Proteus vulgaris ATCC 4636, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 885/653, and Bacillus subtilis ATCC 6633. Authors have applied vector theory for antimicrobial activity comparison of liquorice root extracts.
Results: Phytochemical composition and antimicrobial activity of hydroalcoholic extracts from the liquorice root with the use of different concentrations of ethanol have been studied. The optimal range of ethanol concentration of 65Â±15% v/v has been found. The dependency between the integral index of antimicrobial activity of the extract and concentration of glycyram and licuroside has been found. The minimal concentration of glycyram and licuroside in the extract has been calculated for an exhibition of the target level of extractâ€™s antimicrobial activity, which is 1.0 and 0.11% w/v, respectively.
Conclusion: It has been found that the concentration of licuroside has greater influence on the integral index of extractâ€™s antimicrobial activity in comparison with that one of glycyram. A highly effective technology for obtaining an extract with target phytochemical parameters has been suggested.
2. Asl MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. And its bioactive compounds. Phytother Res 2008;22:709-24.
3. Baltina LA, Kondratenko RM, Baltina LM Jr., Plyasunova OA, Pokrovskii AG, Tolstikov GA. Prospects for creation of new antiviral drugs based on glycyrrhizic acid and its derivatives a review. Pharm Chem J 2009;43:539-48.
4. DelbÃ² M. Assessment Report on Glycyrrhiza glabra L. and or Glycyrrhiza inflate Bat. and or Glycyrrhiza uralensis Fisch., Radix. Report No. EMA/HMPC/571122/2010. London: Committee on Herbal Medicinal Products (HMPC), European Medicines Agency; 2013.
5. Lakshmi T, Geetha R. Glycyrrhiza glabra Linn. Commonly known as Liquorice: A therapeutic review. Int J Pharm Pharm Sci 2011;3:20-5.
6. Langer D, Czarczynska-Gpslinska B, Goslinski T. Glycyrrhetinic acid and its derivatives in infectious diseases. Curr Issues Pharm Med Sci 2016;29:118-23.
7. Shinde D, Koratkar S, Sharma N, Shitole A. Antioxidant activity and antiproliferative action of methanolic extract of liquorice (Glycyrrhiza glabra) in HEPG2 cell line. Int J Pharm Pharm Sci 2016;8:293-8.
8. Gaitry CP, Binda DS, Farhin I, Sujata SD. Antimicrobial and antioxidant activities of methanol extract roots of Glycyrrhiza glabra and HPLC analysis. Int J Pharm Pharm Sci 2013;5:975-1491.
9. Litvinenko VI, Georgievskiy VP, Fursa NS, Ammosov AS, Popova TP. Liquorice: Taxonomy, Chemistry, Technology, Standardization, Pharmacology, Clinic. Yaroslavl: Avers Plus; 2014.
10. Popova NV, Litvinenko VI, Kucanjan AS. Medicinal Plants of the World: Encyclopedic Handbook. Kharkov: Disa Plus; 2016.
11. Tolstikov GA, Baltina LA, Grankina VP, Kondratenko RM, Tolstikova TG. Liquorice: Biodiversity, Chemistry, Application in Medicine. Novosibirsk: Academic Publishing House â€œGeoâ€; 2007.
12. World Health Organization. Monographs on Selected Medicinal Plants. Vol. 1. Geneva: World Health Organization; 1999.
13. Boyko NN, Pisarev D, Zhilyakova E, Novikov O, Kuznietsova V, Sushchuk N, et al. Modeling of solvent effects on phytocompoundsâ€™ extraction from Glycyrrhizae radix. J Adv Pharm Technol Res 2018;9:66-72.
14. European Pharmacopoeia Commission. European Pharmacopoeia. 8th ed. Strasbourg: European Directorate for the Quality of Medicines and HealthCare; 2014.
15. Russian Healthcare Ministry. Russian State Pharmacopoeia. 13th ed. Vol. 2. Moscow: Russian Healthcare Ministry; 2015.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.