ANTIBACTERIAL ACTIVITY OF AMORPHOPHALLUS KONKANENSIS AND AMORPHOPHALLUS BULBIFER TUBER
Objective: Evaluation of antibacterial activity of unexplored, endemic Amorphophallus konkanensis (AKT) Hett., Yadav & Patil and Amorphophallus
bulbifer (ABT) (Roxb.) Bl.
Methods: Acetone and ethanol extracts of tubers of AKT and ABT were evaluated for in vitro antibacterial activity against Bacillus subtilis, Micrococcus
aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae using agar diffusion method, minimum inhibitory
concentration (MIC), minimum bactericidal concentration (MBC) and growth pattern of test organisms. High-performance liquid chromatography
(HPLC) analysis was carried out for active phenolic compounds.
Results: AKT and ABT extracts for both solvents showed significant antibacterial activities. The zone of inhibition was ranged from 12-25 mm to 11-
23 mm for AKT and ABT extracts respectively. Highest significant activity was observed against M. aureus. The MIC values of acetone extracts were
ranged from 200-350 Î¼g/ml (AKT) to, 200-400 Î¼g/ml (ABT). Ethanol extracts of AKT and ABT were showed MIC at 250-450 Î¼g/ml and 300-450 Î¼g/
ml respectively. MBC value of acetone extracts ranged from 260-460 Î¼g/ml (AKT) to 300-500 Î¼g/ml (ABT) and for ethanol extracts 340-500 Î¼g/ml
(AKT) and 380-540 Î¼g/ml (ABT). The effect of extracts on growth of microorganisms was also studied. HPLC analysis showed phenolic compounds
viz. tannic acid, gallic acid, quercetin, p-coumaric acid, and catechin. This polyphenolic rich extracts of AKT and ABT have demonstrated various
degree of microbial growth inhibition.
Conclusion: Antibacterial activity of AKT and ABT implies that, these plants have the potential for preparation of alternative medicines for infections
caused by microorganisms that have become resistant to the current therapeutic measures.
Keywords: Amorphophallus konkanensis, Amorphophallus bulbifer, Antibacterial activity, Minimum inhibitory concentration, minimum bactericidal
concentration, High-performance liquid chromatography.
healt. Nutr Res Rev 2000;13(2):279-99.
2. Jigna P, Sumitra C. Antibacterial and phytochemical studies on twelve
species of Indian medicinal plants. Afr J Biomed Res 2007;10:175-81.
3. Chopra I, Hodgson J, Metcalf B, Poste G. The search for antimicrobial
agents effective against bacteria resistant to multiple antibiotics.
Antimicrob Agents Chemother 1997;41(3):497-503.
4. Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: An expanding
role in development. World Bank Technical Paper, 1996; No. 320.
5. Rehman MM, Wahed MI, Biswas MH, Sadik GM, Haque ME. In vitro
antibacterial activity of the compounds of Trapa bispinosa Roxb.
6. Saswati R, Dutta Choudhury M, Paul SB. Antibacterial activity of
Araceae: An overview. Int J Res Ayur Pharm 2013;4(1):15-7.
7. Alam K, Moizur R, Shariful I. Antibacterial, antifungal and cytotoxic
activities of tuberous roots of Amorphophallus campanulatus. Turk J
8. Majhenic L, Skerget M, Knez Z. Antioxidant and antimicrobial activity
of guarana seed extracts. Food Chem 2007;104:1258-68.
9. Sousa A, Ferreira IC, Calhelha R, Andrade PB, ValentÃ£o P, Seabra R,
et al. Phenolics and antimicrobial activity of traditional stoned table
olives â€˜alcaparraâ€™. Bioorg Med Chem 2006;14(24):8533-8.
10. Raccach M. The antimicrobial acticity of phenolic antioxidants in food:
A review. J Food Saf 1984;6:141-70.
11. Patil KS. Cytotaxonomical and genetical studies in Araceae from
Western Ghats of Maharashtra. Dissertation, Shivaji University,
Kolhapur, MS, India; 1995.
12. Abubakar S, Ahmed Qamar U, Samah Othman A, Omar Muhammad N.
Bacteriostatic and bactericidal activities of Andrographis paniculata
extracts on skin disease causing pathogenic bacteria. J Med Plants Res
13. Kaur GJ, Arora DS. Antibacterial and phytochemical screening of
Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi.
BMC Complement Altern Med 2009;9:30.
14. Farzana K, Batool S, Ismail T, Asad MH, Rasool F, Khiljee S, et al.
Comparative bactericidal activity of various soaps against gram-positive
and gram-negative bacteria. Sci Res Essays 2011;6(16):3514-8.
15. Faras AF, Wadkar SS, Ghosh JS. Effect of leaf extract of Pandanus
amaryllifolius (Roxb.) on growth of Escherichia coli and Micrococcus
(Staphylococcus) aureus. Int Food Res J 2014;21(1):421-3.
16. Bauer R, Tittel G. Quality assessment of herbal preparations as a
precondition of pharmacological and clinical studies. Phytomedicine
17. Springfield EP, Eagles PK, Scott G. Quality assessment of South African
herbal medicines by means of HPLC fingerprinting. J Ethnopharmacol
18. Alonso Paz E, Cerdeiras MP, Fernandez J, Ferreira F, Moyna P,
Soubes M, et al. Screening of Uruguayan medicinal plants for
antimicrobial activity. J Ethnopharmacol 1995;45(1):67-70.
19. Kudi AC, Umoh JU, Eduvie LO, Gefu J. Screening of some Nigerian
medicinal plants for antibacterial activity. J Ethnopharmacol
20. Vlietinck AJ, Van Hoof L, TottÃ© J, Lasure A, Vanden Berghe D,
Rwangabo PC, et al. Screening of hundred Rwandese medicinal
plants for antimicrobial and antiviral properties. J Ethnopharmacol
21. Palombo EA, Semple SJ. Antibacterial activity of traditional Australian
medicinal plants. J Ethnopharmacol 2001;77(2-3):151-7.
22. Chitemerere TA, Stanely M. In vitro antibacterial activity of selected
medicinal plants from Zimbabwe. Afr J Plant Sci Biotechnol
23. Chambers HF. The changing epidemiology of Staphylococcus aureus.
Emerg Infect Dis 2001;7:178-82.
24. Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from
natural sources. J Antimicrob Chemother 2007;59(6):1247-60.
25. Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of
some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu,
India. BMC Complement Altern Med 2006;6:35.
26. Natraj HN, Murthy RL, Ramchandra SS. In vitro screening of
antimicrobial activity and preliminary phytochemical screening of
Suran. Pharmacologyonline 2009;1:189-94.
27. Singh S, Srivastava R, Choudhary S. Antifungal and HPLC analysis
of the crude extracts of Acorus calamus, Tinospora cordifolia and
Celestrus paniculatus. J Agric Sci Technol 2010;6(1): 149-58.
28. Jay JM. Modern Food Microbiology. 5th ed. New York: Chapman &
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.