• Debidas Ghosh Dept. of Bio-Medical Laboratory Science and Management Vidyasagar University,



Objective: Over production of reactive oxygen species is dangerous for all living organisms which can damage the key cellular constituents such as
protein, lipid, and DNA. Recently, searching of different new plant sources having free radical scavenging activity is an interesting field of research in
the pharmaceutical area, as natural products are harmless and low cost. In this connection, we focused to evaluate the antioxidant potential of ethyl
acetate fraction of seed of Eugenia jambolana (E. jambolana) using different in vitro tests.
Methods: To assess the antioxidant activity, said fraction was examined on scavenging of hydrogen peroxide, hydroxyl radical scavenging potential,
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, and anti-lipid peroxidation activity by biochemical methods. We also measured total
phenol and flavonoids contents in the said fraction.
Results: Results indicated that ethyl acetate fraction has strong scavenging activity on hydroxyl radical with inhibitory concentration (IC50) value
54.34 μg/ml, hydrogen peroxide with IC50 value 63.25 μg/ml, DPPH radical with IC50 value 154.95 μg/ml and the said fraction also showed remarkable
inhibition in lipid peroxidation having IC50 value 59.42 μg/ml. In another phytochemical study focused that the fraction is rich in phenolic compounds
(18.54 mg gallic acid equivalent/g dried extract) and flavonoids (122.32 μg quercetin equivalent/g dried extract).
Conclusion: The findings indicated that the ethyl acetate fraction of seed of E. jambolana is a rich source of natural antioxidants.
Keywords: Antioxidant, Eugenia jambolana, Free radicals, Lipid peroxidation.


1. Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med 2008;8:63.
2. Mehran MJ, Zendehbad SH, Sudhakar M. Free radical scavenging and antioxidant potential activity of cassava plants. Asian J Pharm Clin Res 2014;7:66-70.
3. Saritha B, Sumathi K. Studies on antioxidant activity, phenol and flavonoid content of Pisonia alba Span. Asian J Pharm Clin Res 2014;7:106-9.
4. Ellnain-Wojtaszek M, Kruczynski Z, Kasprzak J. Investigation of the free radical scavenging activity of Ginkgo biloba L. leaves. Fitoterapia 2003;74(1-2):1-6.
5. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993;49(3):481-93.
6. Yildirim A, Oktay M, Bulaloulu V. The antioxidant activity of the leaves of Cydonia vulgaris. Turkesh J Med Sci 2001;31:23-7.
7. Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Assimopoulou AN, Boskou D. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem 2006;94:19-25.
8. Pereira JA, Oliveira I, Sousa A, Valentão P, Andrade PB, Ferreira IC, et al. Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food Chem Toxicol 2007;45(11):2287-95.
9. Sarahroodi S, Rasekh HR, Kamalinejad M, Mahboubi S, Taheri Shalmani S, Nouri M. Glucose lowering effect of the water extract of septum of E. jambolana L. (Persian Walnut) fruit in male rats. Pharmacogn Mag 2008;14:109-13.
10. Chatterjee K, Ali KM, Mallick C, Ghosh D. Antihyperglycaemic, antioxidative activities of a formulated polyherbal drug MTEC (Modified) in streptozotocin-induced diabetic rat. J Med Plant Res 2009;3:468-80.
11. Panda DK, Ghosh D, Bhat B, Talwar SK, Jaggi M, Mukherjee R. Diabetic therapeutic effects of ethyl acetate fraction from the roots of Musa paradisiaca and seeds of Eugenia jambolana in streptozotocin-induced male diabetic rats. Methods Find Exp Clin Pharmacol 2009;31(9):571-84.
12. Jana K, Ghosh A, Bera TK, Ghosh D. Evaluation of antidiabetic and antioxidative effect of seed of Eujenia jambolana in streptozotocin induced diabetic male rat. World J Pharm Res 2014;3(6):2040-56.
13. Sharma SB, Nasir A, Prabhu KM, Murthy PS. Antihyperglycemic effect of the fruit-pulp of Eugenia jambolana in experimental diabetes mellitus. J Ethnopharmacol 2006;104(3):367-73.
14. Vaijanathappa J, Badami S, Bhojraj S. In vitro antioxidant activity of Enicostemma axillare. J Health Sci 2008;54:524-8.
15. Badami S, Rai SR, Suresh B. Antioxidant activity of Aporosa lindleyana root. J Ethnopharmacol 2005;101(1-3):180-4.
16. Williams BW, Cuverlier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol Food Sci Technol 1995;28:25-30.
17. Masao H, Yang XW, Miyashrio H, Nabma T. Inhibitory effects of monomeric and dimeric phenyl propanoids from mice on lipid peroxidation in vivo and in vitro. Phytother Res 1993;7:395-401.
18. Sunil C, Latha PG, Suja SR, Shine V, Shyamal SJ, Anuja GI, et al. Effect of ethanolic extract of Pisonia alba Span. leaves on blood glucose levels and histological changes in tissues of alloxan-induced diabetic rats. Int J Appl Res Nat Prod 2009;2:4-11.
19. Trease GE, Evans WC. Pharmacognosy. 15th ed. Uttar Pradesh, India: Elsevier India Pvt. Ltd.; 2008.
20. Zhishen J, Mengchen T, Jianming W. The determination of flavonoids content in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.
21. Singleton VL, Rossi JA. Colometry of total phenolics with phosphomolybodic-phosphotungtic acid reagents. Am J Enol Vitic 1965;6:144-58.
22. Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L. Polyphenols: Food sources and bioavailability. Am J Clin Nutr 2004;79(5):727-47.
23. Marx JL. Oxygen free radicals linked to many diseases. Sci 1987;235(4788):529-31.
24. Braughler JM, Duncan LA, Chase RL. The involvement of iron in lipid peroxidation. Importance of ferric to ferrous ratios in initiation. J Biol Chem 1986;261(22):10282-9.
25. Loew D, Kaszkin M. Approaching the problem of bioequivalence of herbal medicinal products. Phytother Res 2002;16(8):705-11.
26. Sharma US, Kumar A. In vitro antioxidant activity of Rubus ellipticus fruits. J Adv Pharm Technol Res 2011;2(1):47-50.
27. Gautam A, Jhade D, Ahirwar D, Sujane M, Sharma GN. Pharmacognostic evaluation of Toona ciliata bark. J Adv Pharm Technol Res 2010;1(2):216-20.
28. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49(11):5165-70.
29. Paliwal P, Pancholi SS, Patel RK. Pharmacognostic parameters for evaluation of the rhizomes of Curcuma caesia. J Adv Pharm Technol Res 2011;2(1):56-61.
30. Kessler M, Ubeaud G, Jung L. Anti- and pro-oxidant activity of rutin and quercetin derivatives. J Pharm Pharmacol 2003;55(1):131-42.
31. Flora SJ. Role of free radicals and antioxidants in health and disease. Cell Mol Biol (Noisy-le-grand) 2007;53(1):1-2.
32. Chatgilialoglu C, O’Neill P. Free radicals associated with DNA damage. Exp Gerontol 2001;36(9):1459-71.
33. Sinha BN, Sasmal D, Basu SP. Pharmacological studies on Melothria maderaspatana. Fitoterapia 1997;1:75-8.
34. Oyaizu M. Studies on product of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15.
35. Pal D, Mitra S. A preliminary study on the in vitro antioxidant activity of the stems of Opuntia vulgaris. J Adv Pharm Technol Res 2010;1(2):268-72.
36. Oki T, Masuda M, Furuta S, Nishibia Y, Terahara N, Suda I. Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple- fleshed sweet potato cultivars. Food Chem Toxicol 2002;67:1752-6.
37. Liu F, Ng TB Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci 2000;66(8):725-35.
38. Siriwardhana N, Lee KW, Kim SH, Ha JW, Jeon YJ. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Tech Int 2003;9:339-46.
39. Kappus H. Lipid peroxidation-mechanism and biological relevance. In: Aruoma OI, Halliwell B, editors. Free Radicals and Food additives. London, UK: Taylor and Francis; 1991. p. 59-75.
40. Ali KM, Chatterjee K, De D, Bera TK, Mallick C, Ghosh D. Hypoglycemic, antioxidant and antihyperlipidemic effects of the aqueous sepal extracts of Salmalia malabarica in streptozotocin-induced diabetic rat. Ethiop Pharm J 2009;27:1-15.
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How to Cite
JANA, K., A. GHOSH, and D. Ghosh. “IN VITRO ANTIOXIDANT POTENTIAL OF ETHYL ACETATE FRACTION OF SEED OF EUGENIA JAMBOLANA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 1, Jan. 2015, pp. 167-70,
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