ANTICANCER AND FREE RADICAL SCAVENGING POTENTIAL OF THE MARINE ALGICOLOUS ENDOPHYTIC FUNGUS CLADOSPORIUM UREDINICOLA

  • VASAVI THIRUMALANADHUNI Department of Applied Microbiology, Sri Padmavathi Mahilavisvavidyalayam, Tirupati – 517 502, Andhra Pradesh, India.
  • LAVANYA LATHA YERRAGURAVAGARI Department of Applied Microbiology, Sri Padmavathi Mahilavisvavidyalayam, Tirupati – 517 502, Andhra Pradesh, India.
  • VANI MATHAKALA Department of Applied Microbiology, Sri Padmavathi Mahilavisvavidyalayam, Tirupati – 517 502, Andhra Pradesh, India.
  • UMA MAHESWARI DEVI PALEMPALLI Department of Applied Microbiology, Sri Padmavathi Mahilavisvavidyalayam, Tirupati – 517 502, Andhra Pradesh, India.

Abstract

Objective: The objective was to study the antioxidant and anticancer potential of the endophytic fungus Cladosporium uredinicola, isolated from the marine brown alga Dictyota dichotoma.


Methods: Anticancer effect of the endophytic fungal extract was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay using MDA-MB-231 human mammary adenocarcinoma cells as in vitro cancer models. 3T3-L1 pancreatic adipocytes were used as in vitro models for the evaluation of cytotoxic activity against normal cells using MTT assay. Free radical scavenging activity was assessed by 2, 2-diphenyl-1- picrylhydrazyl (DPPH) assay.


Results: The ethyl acetate extract of the endophytic fungus showed potent cytotoxic activity against MDA-MB-231 human breast adenocarcinoma cell lines with an inhibitory concentration (IC50) value of 373 μg/ml and a very mild cytotoxic effect on 3T3-L1 Cells with an IC50 value of 2403 μg/ml. DPPH free radical scavenging assay of the extract showed an IC50 value of 359 μg/ml indicating its potential free radical scavenging activity.


Conclusion: The results indicated that the endophytic fungus C. uredinicola, isolated from the marine brown alga D. dichotoma, acts as a potential source for anticancer and antioxidant metabolites. Moreover, these anticancer metabolites were observed to be less toxic to the normal cells, which make them prospective therapeutic agents.

Keywords: Marine endophytic fungi, Free radical scavenging, Cytotoxic, MDA-MB-231,3T3-L1 cells.

Author Biography

VASAVI THIRUMALANADHUNI, Department of Applied Microbiology, Sri Padmavathi Mahilavisvavidyalayam, Tirupati – 517 502, Andhra Pradesh, India.

Applied Microbiology

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THIRUMALANADHUNI, V., L. L. YERRAGURAVAGARI, V. MATHAKALA, and U. M. D. PALEMPALLI. “ANTICANCER AND FREE RADICAL SCAVENGING POTENTIAL OF THE MARINE ALGICOLOUS ENDOPHYTIC FUNGUS CLADOSPORIUM UREDINICOLA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 520-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30977.
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