• MASOOD A KHAN Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, KSA.


Objective: The present study was aimed to assess the antifungal activity of Tinospora cordifolia aqueous extract (TCAE) against Aspergillus fumigatus infection.

Methods: TCAE was tested for in vitro antifungal activity against the isolates of A. fumigatus, Aspergillus flavus, and Aspergillus niger. To evaluate in vivo activity, various doses (10, 25, and 50 mg/kg) of TCAE were orally administered in A. fumigatus-infected mice for 7 days. The combination of prophylactic and therapeutic effect of TCAE was assessed by pre-treating the mice with 10 mg/kg of TCAE for 3 consecutive days before exposing them to A. fumigatus. Mice were treated with 10, 25, and 50 mg/kg doses of TCAE for 7 consecutive days’ post-A. fumigatus infection. The effectiveness of TCAE was evaluated by monitoring the survival rate and assessing the fungal burden in the kidney of the treated mice.

Results: A. fumigatus-infected mice treated with TCAE at the doses of 25 and 50 mg/kg exhibited 50% and 20% survival rate, respectively, observed on day 40 post-treatment. Like to the survival data, the fungal burden was also found to be the lowest in the kidney of mice treated with TCAE at a dose of 50 mg/kg. The results showed that pre-treatment with TCAE (10 mg/kg) followed by post-infection treatment with 10, 25, and 50 mg/kg of TCAE for 7 days resulted in 40%, 50%, and 70% survival rate, respectively.

Conclusions: These results suggest that TCAE may potentially be considered for its possible use in the treatment of the systemic infection of A. fumigatus.

Keywords: Alternative medicines, Aspergillus fumigatus, Tinospora cordifolia, Fungal infections.

Author Biography

MASOOD A KHAN, Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, KSA.

Assistant Professor


1. Alekshun MN. New advances in antibiotic development and discovery. Expert Opin Invest Drug 2005;14:117-34.
2. Conly J, Rennie R, Johnson J, Farah S, Hellman D. Disseminated candidiasis due to amphotericin B resistant Candida albicans. J Infect Dis 1992;165:761-64.
3. Tujios S, Fontana RJ. Mechanism of drug-induced liver injury: From bedside to bench. Nat Rev Gastroenterol Hepatol 2011;8:202-11.
4. Khan MA, Owais M. Toxicity, stability and pharmacokinetics of amphotericin B in immunomodulator tuftsin-bearing liposomes in a murine model. J Antimicrob Chemother 2006;58:125-32.
5. Chapman SW, Sullivan DC, Cleary JD. In search of the holy grail of antifungal therapy. Trans Am Clin Climatol Assoc 2008;19:197-215.
6. Deepti D, Nupur SS. A study on antioxidant and anti-aging properties of few medicinal plants. Int J Pharm Pharm Sci 2016;8:344-7.
7. Fahrizal AS, Ikbar A. Effect of ethanolic extract of Tinospora crispa L. from Indonesia in alloxan induced liver damage. Int J Pharm Pharm Sci 2017;9:65-8.
8. Sharma U, Bala M, Kumar N, Singh B, Munshi RK, Bhalerao S. Immunomodulatory active compounds from Tinospora cordifolia. J Ethnopharmacol 2012;141:918-26.
9. Thatte UM, Kulkarni MR, Dahanukar SA. Immunotherapeutic modification of Escherichia coli peritonitis and bacteremia by Tinospora cordifolia. J Postgrad Med 1992;38:13-5.
10. Mishra A, Kumar S, Bhargava A, Sharma B, Pandey AK. Studies on in vitro antioxidant and antistaphylococcal activities of some important medicinal plants. Cell Mol Biol 2011;57:16-25.
11. Desai VR, Ramakrishnan R, Chintalwar GJ, Sainis KB. G1-4A, an immunomodulatory polysaccharide from Tinospora cordifolia, modulates macrophage responses and protects mice against lipopolysaccharide induced endotoxic shock. Int Immunopharmacol 2007;7:1375-86.0
12. Gupta PK, Chakraborty P, Kumar S, Singh PK, Rajan MG, Sainis KG, et al. G1-4A, a polysaccharide from Tinospora cordifolia inhibits the survival of mycobacterium tuberculosis by modulating host immune responses in TLR4 dependent manner. PLoS One 2016;11:e0154725.
13. Alsuhaibani S, Khan MA. Immune-stimulatory and therapeutic activity of Tinospora cordifolia: Double-edged sword against salmonellosis. J Immunol Res 2017;2017:1787803.
14. Khan MA, Ahmad N, Moin S, Mannan A, Wajahul H, Pasha ST, et al. Tuftsin-mediated immunoprophylaxis against an isolate of Aspergillus fumigatus shows less in vivo susceptibility to amphotericin B. FEMS Immunol Med Microbiol 2005;44:269-76.
15. Wiederhold NP. Antifungal resistance: Current trends and future strategies to combat. Infect Drug Resist 2017;10:249-59.
16. Khan MA, Aljarbou AN, Khan A, Younus H. Liposomal thymoquinone effectively combats fluconazole-resistant Candida albicans in a murine model. Int J Biol Macromol 2015;76:203-8.
136 Views | 179 Downloads
How to Cite
A KHAN, M. “TINOSPORA CORDIFOLIA AQUEOUS EXTRACT AMELIORATES THE SYSTEMIC INFECTION OF ASPERGILLUS FUMIGATUS IN BALB/C MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Mar. 2019, pp. 525-8, doi:10.22159/ajpcr.2019.v12i3.30984.
Original Article(s)