IN VITRO FREE RADICAL SCAVENGING ACTIVITY AND PHENOL AND FLAVONOID CONTENT OF NERIUM INDICUM, PELTOPHORAM PTEROCARPUM AND ROSA SPPS. FLOWER EXTRACTS
Abstract
Objective: The aim of the present study was to evaluate antioxidant activity and phenol and flavonoid content of three flowers viz. Nerium indicum,
Peltophoram pterocarpum and Rosa spps extracts.
Methods: The extraction was done by individual cold percolation method using solvents like hexane, ethyl acetate and acetone. Total phenolic and
total flavonoid content of different extracts was determined using Folin-ciocalteu assays and aluminium chloride colorimetric method respectively.
Antioxidant activity was carried out by ferric reducing antioxidant power (FRAP) and ABTS radical cation scavenging activity.
Results: Total phenol content was more in acetone extract while total flavonoid content was more in ethyl acetate extract, in all the three flower
species. FRAP activity was maximum in acetone extracts while ABTS activity was more in ethyl acetate extracts.
Conclusion: Phenolic and non-phenolic compounds contribute to the antioxidant activity of these three flower extracts. The best antioxidant activity
was shown by P. pterocarpum flowers suggesting it to be a good source of natural antioxidants.
Keywords: Flowers, Antioxidant activity, 2, 2'-Azino-bis-(3-ethyl) benzothiazoline-6-sulfonic acid, Ferric reducing antioxidant power, Total phenol
content, Total flavonoid content.
Downloads
References
Kumar U, Mishra M, Prakash V. Assessment of antioxidant enzymes
and free radical scavenging activity of selected medicinal plants. Free
Radic Antioxid 2012;2(3):58-63.
Abbasi BH, Stiles AR, Saxena PK, Liu CZ. Gibberellic acid increases
secondary metabolite production in Echinacea purpurea hairy roots.
Appl Biochem Biotechnol 2012;168(7):2057-66.
MartÃnez-Cayuela M. Oxygen free radicals and human disease.
Biochimie 1995;77:147-61.
Zhu X, Raina AK, Lee HG, Casadesus G, Smith MA, Perry G.
Oxidative stress signalling in Alzheimer’s disease. Brain Res
;1000(1-2):32-9.
Yoo MK, Lee HC, Lee H, Moon B, Lee YC. Relative antioxidant
and cytoprotective activities of common herbs. Food Chem
;106:929-36.
Moure A, Cruz JM, Franco D, Domnguez JM, Sineiro J, Domnguez H.
Natural antioxidants from residual sources. Food Chem 2001;72:45-171.
Fusco D, Colloca G, Lo Monaco MR, Cesari M. Effects of
antioxidant supplementation on the aging process. Clin Interv Aging
;2(3):377-87.
Quinming Y, Xianhui P, Weibao K, Hong Y, Yidan S, Zhang L.
Antioxidant activities of malt extract from barley (Hordeum vulgare L.)
towards various oxidative stress in vitro and in vivo. Food Chem
;118:84-9.
Abbasi BH, Khan M, Guo B, Bokhari SA, Khan MA. Efficient
regeneration and antioxidative enzyme activities in Brassica rapavar
turnip. Plant Cell Tissue Organ Cult 2011;105:337-44.
Tosun M, Celik F, Ercisli SO, Yilmaz S. Bioactive contents of
commercial cultivars and local genotypes of walnut (Juglans regia L.).
Int Conf Environ Agric Eng 2011;15:110-4.
Pereira RP, Fachinetto R, de Souza Prestes A, Puntel RL, Santos da
Silva GN, Heinzmann BM, et al. Antioxidant effects of different
extracts from Melissa officinalis, Matricaria recutita and Cymbopogon
citratus. Neurochem Res 2009;34(5):973-83.
Balasundram N, Sundram K, Sammon S Phenolic compounds in plants
and agri-industrial by-products: Antioxidant activity, occurrence, and
potential uses. Food Chem 2006; 99:191–203.
Vinayagam A, Sudha PN. Antioxidant activity of methanolic extracts
of leaves and flowers of Nerium indicum. Int J Pharm Sci Res
;2(6):1548-53.
Anjaria J, Parabia M, Dwivedi S. Ethnovet Heritage Indian
Ethnoveterinary Medicines. An Overview. 1st ed. Ahmedabad, India:
Pathik Enterprise; 2002.
Bairi R, Vijaya C, Ramu A. Evaluation of cardiotonic activity of
Peltophorum pterocarpum. Int J Phytopharmacol 2011;2(1):1-6.
Manaharan T, Teng LL, Appleton D, Ming CH, Masilamani T,
Palanisamy UD. Antioxidant and antiglycemic potential of Peltophorum
pterocarpum plant parts. Food Chem 2011;129:1355-61.
Raj MK, Duraipandiyan V, Agustin P, Ignacimuthu S. Antimicrobial
activity of bergenin isolated from Peltophorum pterocarpum DC.
flowers. Asian Pac J Trop Biomed 2012;901-4.
Kumar N, Bhandari P, Singh B, Bari SS. Antioxidant activity and ultraperformance
LC-electrospray ionization-quadrupole time-of-flight
mass spectrometry for phenolics-based fingerprinting of Rose species:
Rosa damascena, Rosa bourboniana and Rosa brunonii. Food Chem
Toxicol 2009;47(2):361-7.
Baydar NG, Baydar H. Phenolic compounds, antiradical activity
and antioxidant capacity of oil-bearing rose (Rosa damascena Mill.)
extracts. Ind Crops Prod 2013;41:375-80.
Parekh J, Chanda S. In vitro antibacterial activity of the crude methanol
extract of Woodfordia fruticosa kurz. flower (Lythraceae). Braz J
Microbiol 2007;38:204-7.
McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content
and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid
content in propolis by two complementary colorimetric methods.
J Food Drug Anal 2002;10:78-182.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as
a measure of antioxidant powerâ€: The FRAP assay. Ann Biochem
;239:70-6.
Thondre PS, Ryan L, Henry CJ. Barley β-glucan extracts as rich sources
of polyphenols and antioxidants. Food Chem 2011;126:72-7.
Re R, Pellegrini N, Proteggentle A, Pannala A, Yang M, Rice-Evans
C. Antioxidant activity applying an improved ABTS radical cation
decolourisation assay. Free Radic Biol Med 1999; 26(9-10):1231-7.
Zhou HC, Lin YM, Li YY, Li M, Wei SD, Chai WM, et al. Antioxidant
properties of polymeric Proantho cyanidins from fruit stones and
pericarps of Litchi chinenis Sonn. Food Res Int 2011;44:613-20.
Chavan UD, Shahidi F, Naczk M. Extraction of condensed tannins from
beach pea (Lathyrus maritimus L.) as affected by different solvents.
Food Chem 2001;75:509-12.
Kaneria M, Chanda S. Evaluation of antioxidant and antimicrobialcapacity of Syzygium cumini L. leaves extracted sequentially in
different solvents. J Food Biochem 2013;37:168-76.
Franco D, Sineiro J, Rubilar M, Sanchez M. Polyphenols from plant
materials: Extraction and antioxidant power. Electron J Environ Agric
Food Chem 2008;7(8):3210-6.
Rahman H, Manjula K, Anoosha T, Nagaveni K, M. Eswaraiah C,
Bardalai D. In-vitro anti-oxidant activity of Citrullus lanatus seed
extracts. Asian J Pharm Clin Res 2013;6(3):152-7.
Gonzalez M, Gonzalez V. Sample preparation of tropical and subtropical
fruit biowastes to determine antioxidant phytochemicals. Anal Method
;2:1842-66.
Pinelo M, Rubilar M, Jerez M, Sineiro J, Nunez MJ. Effect of solvent,
temperature, and solvent-to-solid ratio on the total phenolic content
and antiradical activity of extracts from different components of grape
pomace. J Agric Food Chem 2005;53:2111-7.
Molehin OR, Adefegha SA. Comparative study of the aqueous and
ethanolic extract of Momordica foetida on the phenolic content and
antioxidant properties. Int Food Res J 2014;21(1):401-5.
Raghavendra M, Reddy AM, Yadav PR, Raju AS, Kumar SL.
Comparative studies on the in vitro antioxidant properties of methanolic
leafy extracts from six edible leafy vegetables of India. Asian J Pharm
Clin Res 2013;6(3):96-9.
Huang MH, Huang SS, Wang RS, Wu CH, Sheu MJ, Hou WC.
Antioxidant and antiinflammatory properties of Cardiospermum
halicacabum and its reference compounds ex vivo and in vivo. J
Ethnopharmacol 2011;133(2):743-50.
Sudha G, Priya MS, Shree RI, Vadivukkarasi S. In vitro free radical
scavenging activity of raw pepino fruit (Solanum muricatum Aiton). Int
J Curr Pharm Res 2011;3(2):137-40.
Shahidi F. Antioxidant extraction identification application and efficacy
measurement. Electron J Environ Agric Food Chem 2008;7:3325-50.
Prior RL, Wu X, Schaich K. Standardized methods for the determination
of antioxidant capacity and phenolics in food and dietry supplements.
J Agric Food Chem 2005;53(10):4290-302.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.