IN VITRO ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF ORMOCARPUM COCHINCHINENSE EXTRACTS ON NOSOCOMIAL INFECTION CAUSING BACTERIA
Objective: The present study was aimed at evaluating the antibacterial activity and phytochemical analysis of the leaf extracts of Ormocarpum
Methods: The leaf powder was extracted by sequential extraction method using chloroform, acetone, and methanol. Preliminary phytochemical
analysis of the extracts was done using standard methods. The dried extracts were screened for antibacterial activity using disk diffusion method and
the minimum inhibitory concentration (MIC) method.
Results: The acetone extract showed the presence of flavonoids, tannins, steroids, phlobatannins, and saponins. Methanol extract contained
flavonoids, tannins, phlobatannins, saponins, and terpenoids. The chloroform extract contained steroids. The chloroform extract did not show any
activity, whereas the acetone extract showed significant activity against all the bacteria with a MIC value of 0.080 mg/ml against Escherichia coli
(ATCC 25922) and Klebsiella pneumoniae (ATCC 15380) and 0.18 mg/ml against Staphylococcus aureus (meticillin resistant). The methanol extract
showed very less activity.
Conclusion: The present study has proved the antibacterial potency of O. cochinchinense for the first time. This plant is a potential source of
antimicrobial agents of the future.
Keywords: Antibacterial activity, Phytochemical analysis, Minimum inhibitory concentration, Ormocarpum cochinchinense, Acetone extract.
Geneva: WHO; 2014.
2. Laboratory Based Surveillance of Antimicrobial Resistance. Report
of Regional Workshop. Chennai, India: World Health Organization
Regional Office for South-East Asia; 2013.
3. Dinesh Kumar M, Maria John KM, Karthik S. The bone fracture â€“
Healing potential of Ormocarpum cochinchinense, methanolic extract
on albino wistar rats. J Herbs Spices Med Plants 2013;19(1):1-10.
4. Sofowora A. Medicinal Plants and Traditional Medicine in Africa.
Ibadan: Spectrum Books Ltd.; 1993.
5. Trease GE, Evans WC. Pharmacognosy. 11th ed. London: Brailliar
Tiridel and Macmillian Publishers; 1989.
6. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques
of Plant Analysis. London: Chapman and Hall, Ltd.; 1973.
7. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility
testing by a standardized single disk method. Am J Clin Pathol
8. Eloff JN. A sensitive and quick microplate method to determine the
minimal inhibitory concentration of plant extracts for bacteria. Planta
9. Uma G, Najila Banu A, Sathica Taj J, Josephine Benedit BU.
Phytochemical screening and antibacterial activity of Scoparia Dulcis
extracts. Asian J Pharm Clin Res 2014;7(3):130-3.
10. Ahameethunisa AR, Hopper W. In vitro antimicrobial activity on
clinical microbial strains and antioxidant properties of Artemisia
parviflora. Ann Clin Microbiol Antimicrob 2012;11:30.
11. Eloff JN. Which extractant should be used for the screening and
isolation of antimicrobial components from plants? J Ethnopharmacol
12. Eloff JN. Quantification the bioactivity of plant extracts during screening
and bioassay guided fractionation. Phytomedicine 2004;11(4):370-1.
13. Chukwujekwu JC, Amoo SO, Van Staden J. Antimicrobial, antioxidant,
mutagenic and antimutagenic activities of Distephanus angulifolius and
Ormocarpum trichocarpum. J Ethnopharmacol 2013;148(3):975-9.
14. Nyandat E, Hassanali A, de Vicente Y, Multari TG, Galeffi C. The
7,7Â²+diglucoside of (2S,3R)-chamaejasmin from Ormocarpum Kirkii.
Pkytockemistry 1990;29 Suppl I:2364-5.
15. Dhooghe L, Maregesi S, Mincheva I, Ferreira D, Marais JP, LemiÃ¨re F, et al.
Antiplasmodial activity of (I-3,II-3)-biflavonoids and other constituents
from Ormocarpum kirkii. Phytochemistry 2010;71(7):785-91.
16. Das S, Borah M, Ahmed S. Antibacterial activity of the ethanolic extract
of leaves of Citrus maxima (Burm.) Merr. on Escherichia coli and
Pseudomonas aeruginosa. Asian J Pharm Clin Res 2013;6(4):136-9.
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