IN VITRO ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF ORMOCARPUM COCHINCHINENSE EXTRACTS ON NOSOCOMIAL INFECTION CAUSING BACTERIA

  • Hannah Hepsibah A Department of Plant Biology and Biotechnology, Loyola College, Chennai.
  • Jeya Jothi G Department of Plant Biology and Biotechnology, Loyola College, Chennai.

Abstract

Objective: The present study was aimed at evaluating the antibacterial activity and phytochemical analysis of the leaf extracts of Ormocarpum
cochinchinense.
Methods: The leaf powder was extracted by sequential extraction method using chloroform, acetone, and methanol. Preliminary phytochemical
analysis of the extracts was done using standard methods. The dried extracts were screened for antibacterial activity using disk diffusion method and
the minimum inhibitory concentration (MIC) method.
Results: The acetone extract showed the presence of flavonoids, tannins, steroids, phlobatannins, and saponins. Methanol extract contained
flavonoids, tannins, phlobatannins, saponins, and terpenoids. The chloroform extract contained steroids. The chloroform extract did not show any
activity, whereas the acetone extract showed significant activity against all the bacteria with a MIC value of 0.080 mg/ml against Escherichia coli
(ATCC 25922) and Klebsiella pneumoniae (ATCC 15380) and 0.18 mg/ml against Staphylococcus aureus (meticillin resistant). The methanol extract
showed very less activity.
Conclusion: The present study has proved the antibacterial potency of O. cochinchinense for the first time. This plant is a potential source of
antimicrobial agents of the future.

Keywords: Antibacterial activity, Phytochemical analysis, Minimum inhibitory concentration, Ormocarpum cochinchinense, Acetone extract.

Author Biographies

Hannah Hepsibah A, Department of Plant Biology and Biotechnology, Loyola College, Chennai.

Research Scholar,

Department of Plant Biology & Biotechnology

Jeya Jothi G, Department of Plant Biology and Biotechnology, Loyola College, Chennai.

Associate Professor,

Department of Plant Biology and Biotechnology,

Loyola College, Chennai.

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How to Cite
Hepsibah A, H., and J. J. G. “IN VITRO ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF ORMOCARPUM COCHINCHINENSE EXTRACTS ON NOSOCOMIAL INFECTION CAUSING BACTERIA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 1, Jan. 2015, pp. 113-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/3231.
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