• ANNAMALAI P Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.
  • BALASHANMUGAM P Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.
  • KALAICHELVAN PT Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.


Objective: The present study was aimed to study at investigating the antibacterial potential of silver nanoparticles (AgNPs) coated cotton fabrics against different pathogens and also for their wound healing property using fibroblasts cells.

Materials and Methods: The leaf extracts of Peltophorum pterocarpum were used for the synthesis of AgNPs and were characterizing using ultraviolet-visible spectrophotometer, transmission electron microscopy, energy dispersive X-ray (EDX) spectroscopy, dynamic light scattering analysis, and zeta potential measurement. The AgNPs were coated on cotton fabrics and tested for their antibacterial efficacy using agar well diffusion method. The wound healing property of synthesized AgNPs was tested using fibroblast 3T3 cells.

Results: The plant extracts of P. pterocarpum were utilized for AgNPs. The optimum condition for synthesizing AgNPs was found to be 1 mg/ml plant concentration, 7 pH, 1 mM silver nitrate concatenation, and incubation temperature of 37°C. The shape of synthesized AgNPs was found to be spherical with an average size between 20 and 50 nm, and elemental silver peaks were confirmed by EDX spectrum. The cotton fabrics coated with AgNPs show good zone of inhibition against all the tested pathogens and the treated fabrics were also characterized using scanning electron microscope which reveals the presence of AgNPs on the fabrics. The scratch assay reveals that the AgNPs have good wound healing activity when tested against fibroblast 3T3.

Conclusion: The present results conclude that the synthesized AgNPs have good stability with potent antimicrobial activity when coated with cotton fabrics. The AgNPs also found to have good activity significant wound healing activity when tested using fibroblast cells.

Keywords: Silver nanoparticles treated cotton fabrics, Transmission electron microscopy, Scratch assay


1. Ali MS, Altaf M, Al-Lohedan HA. Green synthesis of biogenic silver nanoparticles using Solanum tuberosum extract and their interaction with human serum albumin: Evidence of “corona” formation through a multi-spectroscopic and molecular docking analysis. J Photochem Photobiol B 2017;173:108-19.
2. Nakkala JR, Mata R, Gupta AK, Sadras SR. Biological activities of green silver nanoparticles synthesized with Acorus calamus rhizome extract. Eur J Med Chem 2014;85:784-94.
3. Mohapatra B, Kuriakose S, Mohapatra S. Rapid green synthesis of silver nanoparticles and nanorods using Piper nigrum extract. J Alloys Compd 2015;637:119-26.
4. Murugesan S, Bhuvaneswari S, Sivamurugan V. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9:192-7.
5. Ahmed S, Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci 2016;9:1-7.
6. Kulkarni N, Muddapur U. Biosynthesis of metal nanoparticles: A review. J. Nanotechnol 2014;2014:8.
7. Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, et al. Green synthesis of silver nanoparticles using Capsicum annuum L. Extract. Green Chem 2007;9:852-8.
8. Doughari JH. Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents. In Phytochemicals-A Global Perspective of their Role in Nutrition and Health. United Kingdom: Intech Open; 2012.
9. Lo SF, Hayter M, Chang CJ, Hu WY, Lee LL. A systematic review of silver-releasing dressings in the management of infected chronic wounds. J Clin Nurs 2008;17:1973-85.
10. Wani IA, Ahmad T, Manzoor N. Size and shape dependant antifungal activity of gold nanoparticles: A case study of candida. Colloids Surf B Biointerfaces 2013;101:162-70.
11. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, et al. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles.Nanomedicine 2010;6:681-8.
12. Vigneshwaran N, Kumar S, Kathe AA, Varadarajan PV, Prasad V. Functional finishing of cotton fabrics using zinc oxide soluble starch nanocomposites. Nanotechnology 2006;17:5087.
13. Lansdown AB. Silver 2: Toxicity in mammals and how its products aid wound repair. J Wound Care 2002;11:173-7.
14. Xu B, Niu M, Wei L, Hou W, Liu X. The structural analysis of biomacromolecule wool fiber with Ag-loading SiO2 nano-antibacterial agent by UV radiation. J Photochem Photobiol A Chem 2007;188:98-105.
15. Chen X, Schluesener HJ. Nanosilver: A nanoproduct in medical application. Toxicol Lett 2008;176:1-2.
16. Montazer M, Alimohammadi F, Shamei A, Rahimi MK. In situ synthesis of nano silver on cotton using tollens’ reagent. Carbohydr Polym 2012;87:1706-12.
17. Annamalai P, Balashanmugam P, Kalaichelvan P. Biogenic synthesis silver nanoparticles using Peltophorum pterocarpum leaf extracts and its antimicrobial efficacy against selective pathogens. Int J Appl Pharm 2018:10;107-11.
18. Veerasamy R, Xin TZ, Gunasagaran S, Xiang TF, Yang EF, Jeyakumar N, et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc 2011;15:113-20.
19. Balashanmugam P, Kalaichelvan PT. Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. Aqueous extract, and coated on cotton cloth for effective antibacterial activity. Int J Nanomedicine 2015;10 Suppl 1:87-97.
20. Liang CC, Park AY, Guan JL. In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007;2:329-33.
21. Felice F, Zambito Y, Belardinelli E, Fabiano A, Santoni T, Di Stefano R, et al. Effect of different chitosan derivatives on in vitro scratch wound assay: A comparative study. Int J Biol Macromol 2015;76:236-41.
22. Chung IM, Park I, Seung-Hyun K, Thiruvengadam M, Rajakumar G. Plant-mediated synthesis of silver nanoparticles: Their characteristic properties and therapeutic applications. Nanoscale Res Lett 2016;11:40.
23. Sathishkumar G, Gobinath C, Karpagam K, Hemamalini V, Premkumar K, Sivaramakrishnan S, et al. Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L. And its inhibitory activity against human pathogens. Colloids Surf B Biointerfaces 2012;95:235-40.
24. Dipankar C, Murugan S. The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids Surf B Biointerfaces 2012;98:112-9.
25. Khan Z, Hussain JI, Hashmi AA. Shape-directing role of cetyltrimethylammonium bromide in the green synthesis of ag-nanoparticles using neem (Azadirachta indica) leaf extract. Colloids Surf B Biointerfaces 2012;95:229-34.
26. Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart Res 2011;13:2981-8.
27. Rao YS, Kotakadi VS, Prasad TN, Reddy AV, Gopal DV. Green synthesis and spectral characterization of silver nanoparticles from lakshmi tulasi (Ocimum sanctum) leaf extract. Spectrochim Acta A Mol Biomol Spectrosc 2013;103:156-9.
28. Ahmed S, Ikram S. Silver nanoparticles: One pot green synthesis using Terminalia arjuna extract for biological application. J Nanomed Nanotechnol 2015;6:309.
29. Aswathy Aromal S, Philip D. Green synthesis of gold nanoparticles using Trigonella foenum-graecum and its size-dependent catalytic activity. Spectrochim Acta A Mol Biomol Spectrosc 2012;97:1-5.
30. Dubey SP, Lahtinen M, Särkkä H, Sillanpää M. Bioprospective of Sorbus aucuparia leaf extract in development of silver and gold nanocolloids. Colloids Surf B Biointerfaces 2010;80:26-33.
31. Obaid AY, Al-Thabaiti SA, El-Mossalamy EH, Al-Harbi LM, Khan Z. Extracellular bio-synthesis of silver nanoparticles. Arab J Chem 2017;10:226-31.
32. Rahi DK, Parmar AS, Tiwari V. Biosynthesis of silver nanoparticles from fungal root endophytes of Sida acuta plant and evaluation of their antibacterial and antibiotic enhancing activity. Int J Pharm Pharm Sci 2014;6:160-6.
33. Khalil MM, Ismail EH, El-Baghdady KZ, Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem 2014;7:1131-9.
34. Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 2009;32:79-84.
35. Sangeetha J, Sandhya J, Philip J. Biosynthesis and functionalization of silver nanoparticles using Nigella sativa, Dioscorea alata and Ferula asafoetida. Sci Adv Mater 2014;6:1681-90.
36. Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 2011;79:594-8.
37. Das J, Paul Das M, Velusamy P. Sesbania grandiflora leaf extract mediated green synthesis of antibacterial silver nanoparticles against selected human pathogens. Spectrochim Acta A Mol Biomol Spectrosc 2013;104:265-70.
38. Vijayakumar M, Priya K, Nancy FT, Noorlidah A, Ahmed AB. Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Ind Crop Prod 2013;41:235-40.
39. Heydari R, Rashidipour M. Green synthesis of silver nanoparticles using extract of oak fruit hull (jaft): Synthesis and in vitro cytotoxic effect on mcf-7 cells. Int J Breast Cancer 2015;2015:846743.
40. Sukirtha R, Priyanka KM, Antony JJ, Kamalakkannan S, Thangam R, Gunasekaran P, et al. Cytotoxic effect of green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model. Process Biochem 2012;47:273-9.
41. Khatoon N, Mazumder JA, Sardar M. Biotechnological applications of green synthesized silver nanoparticles. J Nanosci Curr Res 2017;2:107.
42. Gao X, Yourick JJ, Topping VD, Black T, Olejnik N, Keltner Z, et al. Toxicogenomic study in rat thymus of F1 generation offspring following maternal exposure to silver ion. Toxicol Rep 2015;2:341-50.
43. Devi LS, Joshi SR. Ultrastructures of silver nanoparticles biosynthesized using endophytic fungi. J Microsc Ultrastruct 2015;3:29-37.
44. Padalia H, Moteriya P, Chanda S. Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arab J Chem 2015;8:732-41.
45. Pannerselvam B, Dharmalingam Jothinathan MK, Rajenderan M, Perumal P, Pudupalayam Thangavelu K, Kim HJ, et al. An in vitro study on the burn wound healing activity of cotton fabrics incorporated with phytosynthesized silver nanoparticles in male Wistar albino rats. Eur J Pharm Sci 2017;100:187-96.
46. Hebeish A, El-Naggar ME, Fouda MM, Ramadan MA, Al-Deyab SS, El-Rafie MH. Highly effective antibacterial textiles containing green synthesized silver nanoparticles. Carbohydr Polym 2011;86:936-40.
47. Wu M, Ma B, Pan T, Chen S, Sun J. Silver-nanoparticle-colored cotton fabrics with tunable colors and durable antibacterial and self-healing superhydrophobic properties. Adv Funct Mater 2016;26:569-76.
48. Sana SS, Badineni VR, Arla SK, Boya VK. Eco-friendly synthesis of silver nanoparticles using leaf extract of Grewia flaviscences and study of their antimicrobial activity. Mater Lett 2015;145:347-50.
49. El-Rafie MH, Ahmed HB, Zahran MK. Characterization of nanosilver coated cotton fabrics and evaluation of its antibacterial efficacy. Carbohydr Polym 2014;107:174-81.
50. El-Shishtawy RM, Asiri AM, Abdelwahed NA, Al-Otaibi MM. In situ production of silver nanoparticle on cotton fabric and its antimicrobial evaluation. Cellulose 2011;18:75-82.
51. Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM. Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224. J Mater Res Sci Technol 2008;24:192-6.
52. Zahran MK, Ahmed HB, El-Rafie MH. Surface modification of cotton fabrics for antibacterial application by coating with agNPs-alginate composite. Carbohydr Polym 2014;108:145-52.
53. Tian J, Wong KK, Ho CM, Lok CN, Yu WY, Che CM, et al. Topical delivery of silver nanoparticles promotes wound healing. ChemMedChem 2007;2:129-36.
54. Neibert K, Gopishetty V, Grigoryev A, Tokarev I, Al-Hajaj N, Vorstenbosch J, et al. Wound-healing with mechanically robust and biodegradable hydrogel fibers loaded with silver nanoparticles. Adv Healthc Mater 2012;1:621-30.
55. Liu X, Lee PY, Ho CM, Lui VC, Chen Y, Che CM, et al. Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing. ChemMedChem 2010;5:468-75.
57 Views | 20 Downloads
How to Cite
Original Article(s)