• Mercy Lavanya S Captain Srinivasa Murti Drug Research Institute for Ayurveda
  • Gnanamani A Central Leather Research Institute
  • Ilavarasan R Captain Srinivasa Murti Drug Research Institute for Ayurveda



Objective: The plant Orthosiphon thymiflorus (Roth.) sleesen is an herb belonging to the family Lamiaceae. It has been reported to possess
several pharmacological properties. The study was aimed at carrying out the physico-chemical, phytochemical and high-performance thin layer
chromatography (HPTLC) analysis on the whole plant of O. thymiflorus.
Methods: The physico-chemical parameters were tested using standard methods. The preliminary tests for phytochemicals present were tested
using conventional methods. CAMAG HPTLC system equipped with TLC autosampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software was
used for HPTLC analysis of the plant. The different extracts were developed using suitable mobile phases using standard procedures and scanned
under ultraviolet 254 nm. The plates were also viewed under 366 nm and after derivatization with vanillin-sulfuric acid.
Results: The physico-chemical parameters were studied. The plant was found to be free from adulteration and contamination. On phytochemical
analysis, the plant showed the presence of triterpenoid, flavonoid, alkaloid, phenol, steroid, sugars, quinone and coumarin in different extracts tested.
The TLC plate showed several spots at different Rf when viewed under 254 nm, 366 nm and after derivatization with vanillin-sulfuric acid. The HPTLC
profile showed several peaks that indicated the presence of various phytochemicals present in the plant.
Conclusion: The study will help in standardization of the plant. The HPTLC profile will help in authentication and standardization of the plant.
Keywords: Orthosiphon thymiflorus, High performance thin layer chromatography, Phytochemical screening, Standardization.

Author Biographies

Mercy Lavanya S, Captain Srinivasa Murti Drug Research Institute for Ayurveda


Gnanamani A, Central Leather Research Institute


Ilavarasan R, Captain Srinivasa Murti Drug Research Institute for Ayurveda



Vaidyaratnam PS. Indian Medicinal Plants – A Compendium of 500 Species. Vol. 4. Kottakkal: Orient Longman Limited; 1997. p. 191‑2.

Pandey G. Uncommon Plant Drugs of Ayurveda. Delhi: Indian Book Centre, Sri Satguru Publications; 1994. p. 62-8.3. Kovendan K, Murugan K, Vincent S, Barnard DR. Mosquito larvicidal properties of Orthosiphon thymiflorus (Roth) Sleesen. (Family: Labiatae) against mosquito vectors, Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Asian Pac J Trop Med 2012;5(4):299-305.

Kavimani S, Ilango R, Thangadurai JG, Jaykar B, Majumdar UK, Gupta M. Diuretic activity of aqueous extract of Orthosiphon thymiflorus in rats. Indian J Pharm Sci 1997;59(2):96-8.

Sundarammal S, Thirugnanasampandan R, Tamil Selvi M. Chemical composition analysis and antioxidant activity evaluation of essential oil from Orthosiphon thymiflorus (Roth) Sleesen. Asian Pac J Trop Biomed 2012;2(1):S112-5.

Pawar RK, Shivani S, Singh KC, Sharma Rajeev KV. Physico-chemical standardization and development HPTLC method for the determination of andrographonin in Kalmgh Navyas Loha. An ayurvedic formulation. Int J Res Ayurveda Pharm 2011;2(1):295-301.

Mukharjee PK. Quality Control of Herbal Drugs. New Delhi: Business Horizons Pharmaceutical Publishers; 2008. p. 186.

Indian Herbal Pharmacopoeia. Indian Drug Manufacturers’ Association. Revised edition. Mumbai: Indian Herbal Pharmacopoeia; 2002. p. 493‑4.

World Health Organization. Quality Control Methods for Medicinal Plants: Determination of Microorganisms. Geneva: World Health Organization; 1998. p. 64-73.

Harborne JB. Phytochemical Methods. 2nd ed. London: Chapman & Hall; 1973.

Kokate CK. Practical Pharmacognosy. 4th ed. Delhi: Vallabh Prakashan; 1997. p. 107-11.

Sethi PD. High Performance Thin Layer Chromatography. 1st ed., Vol. X. New Delhi: CBS Publishers and Distributors; 1996.

Stahl I. Thin Layer Chromatography. A Laboratory Hand Book. Student edition. Berlin: Springer-Verlag; 1969.

Wagner H, Baldt S, Zgainski EM. Plant Drug Analysis. Berlin: Springer; 1996.

Sharma PK, Mohammad A, Yadav DK. Physico-chemical and phytochemcial evaluation of different tea brands. J Appl Pharm Sci 2011;1(03):121-4.

Sridevi N, Changam SS, Cherian KM. Extraction and preliminary phytochemical screening of active compounds in Morinda citrifolia Fruit. Asian J Pharm Clin Res 2012;5(2):179-81.

Hariprasad PS, Ramakrishnan N. HPTLC fingerprint profile of Rumex vesicarius L. Asian J Pharm Clin Res 2011;4(2):134-6.



How to Cite

S, M. L., G. A, and I. R. “PHYSICO-CHEMICAL, PHYTOCHEMICAL AND HPTLC ANALYSIS OF THE WHOLE PLANT OF ORTHOSIPHON THYMIFLORUS (ROTH.) SLEESEN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 1, Jan. 2015, pp. 181-4,



Original Article(s)